Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02806-0005-0000-3
Chemical Name:	Sodium (2-ethylhexyl)alcohol sulfate
CASRN:	126-92-1
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	647561
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	137r	110	155	38	42
			108	101	138	43
			111	99	119	36
			99	100	138	46
Test Chemical	Sodium (2-ethylhexyl)alcohol sulfate	156.25	111	105	164	49	43
			106	101	115	36	43
		312.5	92	99	138	50	47
			116	89	156	45	47
		625	90	94	150	56	54
			106	86	166	52	54
		1250	105	86	233	74	70*
			93	80	186	67	70*
		2500	104	75	166	53	60
			104	66	207	66	60
Positive Control	Methyl Methane Sulfonate	15	79	40	415	175	183*
			68	34	388	190	183*
	Methyl Methane Sulfonate	250	105	94	602	191	201*
			95	79	598	211	201*

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	79	103	95	40	42
			84	101	127	51
			87	106	128	49
			82	91	65	26
Test Chemical	Sodium (2-ethylhexyl)alcohol sulfate	200	82	102	121	49	47
			83	108	112	45	47
		1000	83	78	145	59	42
			61	12	47	26	42
		1800	79	63	102	43	43
			84	67	109	44	43
		2600	78	44	145	62	53
			74	28	100	45	53
		3400	64	18	91	47	53
			70	21	124	59	53
		4200

Positive Control	Methyl Methane Sulfonate	20	9r	4	141	553	529*
			11	4	180	529	529*
	Methyl Methane Sulfonate	250	61	78	554	302	301*
			64	87	573	299	301*

Nonactivation Trial: 3 Experiment Call: Rejected
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	77	96	56	24	18
			67	90	70	35
			72	115	15	7
			76	99	15	7
Test Chemical	Sodium (2-ethylhexyl)alcohol sulfate	200	64	85	79	41	35*
			62	85	53	28	35*
		1000	65	58	57	29	34*
			64	57	74	39	34*
		1800	68	55	71	35	34*
			62	57	62	34	34*
		2600	69	44	100	48	48*
			68	38	97	48	48*
		3400	65	30	102	52	51*
			64	20	95	50	51*
		4200

Positive Control	Methyl Methane Sulfonate	20	4r	1	44	400
Positive Control			5r	1	51	364

Nonactivation Trial: 4 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	82	93	81	33	28
			81	110	53	22
			96	109	45	16
			86	88	110	43
Test Chemical	Sodium (2-ethylhexyl)alcohol sulfate	1000	56	46	93	55	53*
			68	58	103	51	53*
		1800	44	33	91	69	59*
			57	40	84	49	59*
		2600	54	37	141	87	71*
			56#	38	93	55	71*
		3400	69	25	99	48	58*
			62	17	124	67	58*
		4200

Positive Control	Methyl Methane Sulfonate	15	33	22	199	204	191*
Positive Control			34	27	181	177	191*

Nonactivation Trial: 5 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	93	113	78	28	29
			78	93	76	33
			93	102	83	30
			79	91	57	24
Test Chemical	Sodium (2-ethylhexyl)alcohol sulfate	1000	84	95	61	24	26
			90	78	75	28	26
		1800	74	85	49	22	25
			73	82	59	27	25
		2600	80	65	56	23	24
			69	76	51	25	24
		3400	84	52	48	19	19
			69	55	40	19	19
		4200	73	14	78	36	26
			82	13	39	16	26
		5000

Positive Control	Methyl Methane Sulfonate	15	39	31	320	277	313*
Positive Control			25	27	258	349	313*

Induced S9 Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	95	111	111	39	31
			87	97	105	40
			82	113	64	26
			100	79	52	17
Test Chemical	Sodium (2-ethylhexyl)alcohol sulfate	200	42	53	89	70	50
			61	99	56	31	50
		1000	47	61	66	46
		1800	46	58	23	17	13
			50	55	14	9	13
		2600	50	44	22	15	15
			49	47	21	14	15
		3400	42	24	16	13	15
			48	20	24	17	15
		4200

Positive Control	3-Methylcholanthrene	2.5	83	85	384	154	157*
Positive Control			75	81	361	160	157*

Induced S9 Trial: 2 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	85	102	97	38	39
			98	107	103	35
			82	85	103	42
			85	106	104	41
Test Chemical	Sodium (2-ethylhexyl)alcohol sulfate	1000	101	113	80	26	31
			94	101	99	35	31
		1800	75	89	71	32	38
			89	96	120	45	38
		2600	104	87	117	38	33
			87	88	73	28	33
		3400	86	73	81	31	34
			83	72	89	36	34
		4200	92	54	92	33	35
			97	56	109	38	35
Positive Control	3-Methylcholanthrene	2.5	79	59	642	271	266*
Positive Control			63	51	496	262	266*

Induced S9 Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	80	96	108	45	47
			89	97	126	47
			68	83	110	54
			97	123	118	40
Test Chemical	Sodium (2-ethylhexyl)alcohol sulfate	1000	75	91	122	55	56
			68	84	115	57	56
		1800	71	80	164	77	72
			65	77	129	66	72
		2600	85	71	190	75	71
			72	70	144	67	71
		3400	70	20	161	77	73
			63	17	129	69	73
		4200

Positive Control	3-Methylcholanthrene	2.5	51	26	536	353	384*
Positive Control			42	21	523	415	384*

Induced S9 Trial: 4 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	69	98	108	53	60
			78	95	134	57
			69	103	131	63
			81	104	162	67
Test Chemical	Sodium (2-ethylhexyl)alcohol sulfate	2600	73	69	150	68	65
			70	58	127	61	65
		3000	72	60	110	51	51
			67	66	103	51	51
		3400	82	59	116	47	49
			67	45	100	50	49
		3800	56	25	83	50	54
			46	23	82	59	54
		4200	64	44	97	51	53
			67	33	111	55	53
Positive Control	3-Methylcholanthrene	2.5	47	27	605	432	519*
Positive Control			31	22	560	605	519*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination