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Study Information
Study Attribute Value
CEBS Accession Number: 002-02811-0001-0000-5
Chemical Name: Sodium bifluoride
CASRN: 51273-71-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 113452
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 82 90 196 80 73
    103 112 196 64
    128r 135 276 72
    105 98 238 76
Test Chemical Sodium bifluoride 
40         
 92 108 193 70 71
    111 96 236 71
  
120         
 94 64 257 92 89
    78 63 203 87
  
200         
 86 36 315 123 114*
    90 37 285 105
  
280         
 78 23 296 126 128*
    84 26 326 130
  
360         
 70 10 331 158 161*
    58 14 286 165
  
420         
   
Positive Control Methyl Methane Sulfonate 
15         
 45 24 429 317 336*
    41 26 440 356

Nonactivation Trial: 2 Experiment Call: Equivocal
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 113 103 109 32 43
    91 94 144 53
    110 96 168 51
    91 107 102 37
Test Chemical Sodium bifluoride 
225         
 101 49 148 49 48
    101 69 141 47
  
275         
 94 47 128 45 43
    108 53 134 41
  
325         
 76 39 129 56 50
    92 34 121 44
  
375         
 98 37 167 57 57
    98 35 167 57
  
425         
 94 27 165 58 71*
    89 12 225 85
Positive Control Methyl Methane Sulfonate 
15         
 32 18 433 449 533*
    27 14 497 617

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 63 99 130 69 63
    46r 69 90 66
    73 93 128 59
    71 108 131 62
Test Chemical Sodium bifluoride 
350         
 59 41 249 141 132*
    74 33 271 123
  
400         
 56 35 220 131 133*
    95 23 384 134
  
450         
 50 23 208 138 153*
    61 23 309 168
  
500         
 58 16 314 180 196*
    42 15 269 212
  
550         
 47 11 274 193 177*
    61 10 296 161
Positive Control Methyl Methane Sulfonate 
15         
 16 10 397 810 793*
    20 13 466 777

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination