Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02811-0001-0000-5
Chemical Name:	Sodium bifluoride
CASRN:	51273-71-3
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	113452
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	82	90	196	80	73
			103	112	196	64
			128r	135	276	72
			105	98	238	76
Test Chemical	Sodium bifluoride	40	92	108	193	70	71
			111	96	236	71	71
		120	94	64	257	92	89
			78	63	203	87	89
		200	86	36	315	123	114*
			90	37	285	105	114*
		280	78	23	296	126	128*
			84	26	326	130	128*
		360	70	10	331	158	161*
			58	14	286	165	161*
		420

Positive Control	Methyl Methane Sulfonate	15	45	24	429	317	336*
Positive Control			41	26	440	356	336*

Nonactivation Trial: 2 Experiment Call: Equivocal
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	113	103	109	32	43
			91	94	144	53
			110	96	168	51
			91	107	102	37
Test Chemical	Sodium bifluoride	225	101	49	148	49	48
			101	69	141	47	48
		275	94	47	128	45	43
			108	53	134	41	43
		325	76	39	129	56	50
			92	34	121	44	50
		375	98	37	167	57	57
			98	35	167	57	57
		425	94	27	165	58	71*
			89	12	225	85	71*
Positive Control	Methyl Methane Sulfonate	15	32	18	433	449	533*
Positive Control			27	14	497	617	533*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	63	99	130	69	63
			46r	69	90	66
			73	93	128	59
			71	108	131	62
Test Chemical	Sodium bifluoride	350	59	41	249	141	132*
			74	33	271	123	132*
		400	56	35	220	131	133*
			95	23	384	134	133*
		450	50	23	208	138	153*
			61	23	309	168	153*
		500	58	16	314	180	196*
			42	15	269	212	196*
		550	47	11	274	193	177*
			61	10	296	161	177*
Positive Control	Methyl Methane Sulfonate	15	16	10	397	810	793*
Positive Control			20	13	466	777	793*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination