Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02811-0002-0000-6
Chemical Name:	Sodium bifluoride
CASRN:	51273-71-3
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	130535
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	86#	103	82	32	27
			87	104	63	24
			83	93	61	24
Test Chemical	Sodium bifluoride	25	84	73	51	20	38
			75	82	56	25
			45	41	92	68
		50	65#	75	47	24	38
			65	84	79	41
			93	51	133	48
		100	67#	63	46	23	20
			95	93	49	17	20
		200	77	46	82	35	29
			79	50	72	31
			87	62	53	20
		300	94#	42	163	58	54*
			72	23	134	62
			69#	38	88	42
		400	71#	16	122	57	69*
			66#	3	159	80	69*
		500


Positive Control	Methyl Methane Sulfonate	5	74	62	480	218	200*
			90#	58	472	175
			84	64	516	206

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	70	90	43	20	16
			76#	108	30	13
			76	98	38	17
			97	104	45	15
Test Chemical	Sodium bifluoride	25	34	42	100	97	77*
			30	36	74	83
			56	55	88	52
		50	44	60	62	47	53*
			66	92	71	36
			33	42	76	76
		100	62	70	83	44	44*
			66	83	61	31
			61	54	102	56
		200	48	45	86	59	66*
			72	49	136	63
			52	48	116	75
		300	54	21	59	36	43*
			68	18	113	55
			57	23	64	37
		400	67	6	110	54	132*
			30	2	162	180
			42	3	205	161
		500


Positive Control	Methyl Methane Sulfonate	5	65	57	474	245	220*
			58	44	162	92
			46	45	444	324

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0					18
			104	64	56	18
			123r	109	88	24
			112	136	64	19
Test Chemical	Sodium bifluoride	100	98	94	77	26	29
			107	69	92	29
			120	129	111	31
		200	114	73	108	32	36*
			107	61	111	35
			116	77	141	41
		250	106	53	164	51	42*
			110	87	93	28
			101	67	144	47
		300	105	36	148	47	42*
			105	46	110	35
			87	31	118	45
		350	108	14	204	63	54*
			116	23	206	59
			98	14	115	39
		400					43*
			115	29	114	33
			99#	7	156	53
		500


Positive Control	Methyl Methane Sulfonate	5	98	39	874	299	277*
			83	26	710	284
			104	76	773	249

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination