Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02814-0007-0000-4
Chemical Name:	Sodium chloride
CASRN:	7647-14-5
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	681631
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	70	99	87	41	39
			75	91	84	38
			98	129	123	42
			72	81	73	34
Test Chemical	Sodium chloride	312.5	73	84	103	47	57
			50	62	101	67	57
		625	98	106	149	51	53
			55	86	93	56	53
		1250	79	92	104	44	49
			66	82	106	54	49
		2500	84	89	138	55	58
			70	80	130	62	58
		5000	68	56	119	59
			121r	81	190	52
Positive Control	Methyl Methane Sulfonate	15	27	28	362	441	382*
Positive Control			43	26	412	322	382*

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	55	115	142	86	80
			37r	106	77	70
			37r	73	79	72
			52	85	118	75
Test Chemical	Sodium chloride	1000	43	91	122	94	83
			43	93	93	72	83
		2000	47	84	119	84	75
			39	70	76	65	75
		3000	51	73	103	67	70
			45	81	97	72	70
		4000	42	78	122	96	88
			44	67	105	79	88
		5000	34	67	51	50	65
			40	51	94	79	65
Positive Control	Methyl Methane Sulfonate	15	5r	3	106.5	666
Positive Control			5r	3	111	716

Nonactivation Trial: 3 Experiment Call:
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	102	100	65	21	23
			93	99	35	13
			99	87	95	32
			87	114	64	24
Test Chemical	Sodium chloride	3000	120	66	41	11	14
			89	79	46	17	14
		4000	118	65	71	20	17
			100	67	39	13	17
		5000	103	43	53	17	16
			100	43	47	16	16
		6000	102	13	74	24	26
			110	13	91	28	26
		7000

Positive Control	Methyl Methane Sulfonate	15	51	31	107	70	72*
Positive Control			51	27	112	74	72*

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	70	110	105	50	52
			75	107	102	45
			66	109	95	48
			87	74	170	65
Test Chemical	Sodium chloride	1000	89	121	138	52	52
			77	110	120	52	52
		2000	68	117	98	48	43
			73	103	83	38	43
		3000	92	106	116	42	46
			75	100	112	50	46
		4000	68	94	102	50	48
			80	92	109	45	48
		5000	86	74	134	52	60
			78	71	160	68	60
Positive Control	3-Methylcholanthrene	2.5	57	27	591	344	371*
Positive Control			52	31	618	397	371*

Induced S9 Trial: 3 Experiment Call: Negative and Non-Toxic
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	50r	79	55	37	25
			49r	81	59	40
			73	114	35	16
			55	86	56	34
Test Chemical	Sodium chloride	2000	33	79	56	56	39
			46	79	29	21	39
		3000	37	56	46	41	31
			61	88	38	21	31
		4000	51	64	64	42	36
			46	57	42	30	36
		5000	52	76	53	34	32
			46	67	42	30	32
Positive Control	3-Methylcholanthrene	2.5	27	33	253	314	380*
Positive Control			30	37	396	445	380*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination