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Study Information
Study Attribute Value
CEBS Accession Number: 002-02814-0008-0000-5
Chemical Name: Sodium chloride
CASRN: 7647-14-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 920073
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 89 122 71 27 28
    90 98 108 40
    78 88 62 27
    87 92 53 20
Test Chemical Sodium chloride 
1000         
 83 89 64 26 24
    79 73 62 26
    89 88 50 19
  
2000         
 79 60 72 30 30
    52 63 62 40
    87 71 54 21
  
3000         
 95# 70 103 36 37
    79 68 88 37
    60 52 67 37
  
4000         
 78 35 144 62 57*
    98 49 140 48
    83 35 154 62
  
5000         
 70 19 168 81 134*
    52 5 300 194
    67 11 254 126
  
6000         
   
   
Positive Control Ethyl Methane Sulfonate 
250         
 73 41 903 412 452*
    61 35 816 443
    56 30 841 501

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 79 98 86 36 40
    74# 98 88.5 40
    83 93 116 47
    95 110 110 39
Test Chemical Sodium chloride 
1000         
 57 72 80 47 36
    53 63 49 31
    67 80 59 29
  
2000         
 94 119 82 29 33
    54 57 61 38
  
3000         
 54 63 73 45 45
    61 67 92 50
    60 54 74 41
  
4000         
 70 54 96 45 53
    59 41 107 61
  
5000         
 62 34 149 80 67*
    71 53 118 55
    69 43 137 66
  
6000         
 71 36 169 79 75*
    66 33 186 94
    62# 36 96 51
Positive Control Ethyl Methane Sulfonate 
250         
 64# 58 828 429 426*
    57 50 751 442
    64 49 776 406

Induced S9 Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 115# 99 204 59 58
    111 100 198 60
    100 101 167 56
Test Chemical Sodium chloride 
500         
 83 52 150 60 59
    75 52 128 57
    95 44 171 60
  
1000         
 98 70 128 44 47
    93 56 142 51
  
2000         
 90# 47 201 75 67
    94 48 175 62
    90# 51 172.5 64
  
3000         
 79# 47 144 61 67
    89 31 168 63
    87 38 202 77
  
4000         
 97# 25 219 75 81
    85 20 216 85
    76 17 190 84
  
5000         
 88 18 234 89 79
    83 15 172 69
Positive Control 3-Methylcholanthrene 
2.5       
 68# 39 650 319 354*
    76 47 779 342
    70 37 844 403

Induced S9 Trial: 2 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 108 93 264 81 81
    94 87 226 80
    115 120 276 80
    130r 121 284 73
Test Chemical Sodium chloride 
500         
 109 90 236 72 65
    82 70 151 61
    94 90 169 60
  
1000         
 129r 97 263 68
    77 74 252 109
    127r 100 272 72
  
2000         
 120 82 316 88 86
    101# 76 284 94
    104 86 236 75
  
3000         
 109 62 209 64 77
    77 56 235 102
    110 66 213 65
  
4000         
 106 85 302 95 93
    111 77 269 81
    93 62 287 103
  
5000         
 93 46 304 109 123*
    101 51 302 100
    70 43 334 159
Positive Control 3-Methylcholanthrene 
2.5       
 117 38 1011 289 295*
    87 44 757 292
    95 41 866 304

Induced S9 Trial: 3 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 115 120 165 48 52
    130r 121 161 41
    108 93 183 56
    94 87 143 51
Test Chemical Sodium chloride 
500         
 94 90 114 41 42
    109 90 145 44
    82 70 105 43
  
1000         
 129r 97 161 42
    77 74 150 65
    127r 100 170 45
  
2000         
 120 82 189 53 52
    101# 76 173 57
    104 86 143 46
  
3000         
 109 62 131 40 45
    77 56 132 57
    110 66 127 39
  
4000         
 106 85 191 60 58
    111 77 166 50
    93 62 179 64
  
5000         
 93 46 193 69 76
    101 51 195 64
    70 43 199 95
Positive Control 3-Methylcholanthrene 
2.5       
 95 41 637 224 209*
    117 38 716 205
    87 44 518 200

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination