Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02820-0008-0000-2
Chemical Name:	Sodium diethyldithiocarbamate
CASRN:	148-18-5
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	456551
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	100	117	108	36	46
			75	83	127	56
			87	95	130	50
			74	106	97	43
Test Chemical	Sodium diethyldithiocarbamate	0.3125	67	53	240	119	113*
			74	57	238	107	113*
		0.625	77	71	257	111	109*
			61	61	196	107	109*
		1.25	75	82	134	60	68
			76	67	175	76	68
		2.5	74	54	84	38	58
			59	48	141	79	58
		5	37	4	347	315	284*
			37	4	282	252	284*
		10

Positive Control	Methyl Methane Sulfonate	20	37	27	352	314	342*
			31	19	342	370	342*
	Methyl Methane Sulfonate	250	64	64	464	243	277*
			60	67	558	311	277*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	88	105	92	35	38
			85	115	86	34
			84	99	90	36
			83	82	117	47
Test Chemical	Sodium diethyldithiocarbamate	0.5	67	29	281	140	156*
			66	25	339	172	156*
		1	68	60	201	98	99*
			51	39	153	99	99*
		2	49	32	208	143	155*
			58	36	291	168	155*
		3	53	10	521	328	364*
			45	8	539	401	364*
		4

Positive Control	Methyl Methane Sulfonate	20	7r	3	88	409	266*
Positive Control			13	5	101	266	266*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	82	92	129	52	48
			70	106	107	51
			53	88	83	53
			80	113	91	38
Test Chemical	Sodium diethyldithiocarbamate	0.063	34	14	104	103	133*
			40	19	196	163	133*
		0.125	44	22	230	173	163*
			37	21	172	154	163*
		0.25	46	35	210	152	161*
			40	29	203	169	161*
		0.5	50	41	214	142	165*
			53	40	300	188	165*
		1	64	51	184	96	85*
			49	78	109	74	85*
Positive Control	Methyl Methane Sulfonate	15	20	14	242	414	498*
Positive Control			24	18	413	582	498*

Nonactivation Trial: 4 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	58	106	91	52	41
			69	107	84	40
			67	93	64	32
			62	94	71	38
Test Chemical	Sodium diethyldithiocarbamate	0.031	46	25	124	90	95*
			54	51	163	100	95*
		0.063	40	11	194	163	195*
			48	13	326	228	195*
		0.125	39	11	213	182	185*
			47	12	265	188	185*
		0.25	56	14	213	126	131*
			73	18	299	137	131*
		0.5	54	22	211	131	126*
			65	28	239	122	126*
		1	58	26	178	103	101*
			62	31	184	99	101*
Positive Control	Methyl Methane Sulfonate	15	18	13	149	271	239*
Positive Control			21	18	128	206	239*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination