Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02821-0005-0000-0
Chemical Name:	Sodium dodecyl sulfate
CASRN:	151-21-3
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	333852
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	80	112	153	64	43
			65	95	66	34
			68	98	90	44
			62	95	56	30
Test Chemical	Sodium dodecyl sulfate	3.125	59	97	151	85	61
			77	106	84	36	61
		6.25	73	107	160	74	78*
			71	119	176	83	78*
		12.5	67	133	160	80	65
			90	145	133	49	65
		25	65	88	130	67	83*
			84	90	247	99	83*
		50	76	98	145	64	69*
			86	82	192	75	69*
		100

Positive Control	Methyl Methane Sulfonate	15	25	23	219	298	232*
Positive Control			27	21	135	167	232*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	61	87	83	45	48
			86	106	115	45
			74	108	119	53
			80	100	115	48
Test Chemical	Sodium dodecyl sulfate	10	66	94	137	69	58
			75	112	107	47	58
		20	53	76	70	44	52
			67	89	119	59	52
		30	64#	86	144	75	60
			71	70	98	46	60
		40	77#	77	126	54
		50	68	56	163	80	74
			94	61	191	68	74
		60	77	16	595	256	203*
			81#	27	364.5	150	203*
		70

Positive Control	Methyl Methane Sulfonate	15	34	30	683	666	664*
Positive Control			29	27	573	662	664*

Nonactivation Trial: 3 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	74	96	58	26	34
			82	97	98	40
			71	104	73	34
			76	103	84	37
Test Chemical	Sodium dodecyl sulfate	50	75	75	82	36	36
			60	71	65	36	36
		55	68	44	68	33	38
			53	71	69	44	38
		60	72	74	134	62	56*
			72	63	107	50	56*
		65	64	71	87	45	42
			79#	68	93	39	42
		70	80	83	107	44
Positive Control	Methyl Methane Sulfonate	15	26	23	156	197	158*
Positive Control			36	26	127	119	158*

Nonactivation Trial: 4 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	93	62	158	57	37
			99	74	62	21
			108	149	112	35
			103#	115	112.5	36
Test Chemical	Sodium dodecyl sulfate	30	96	94	107	37	31
			94	109	72	26	31
		40	103	72	137	44	41
			104	86	119	38	41
		50	104	77	117	37	38
			94	61	107	38	38
		60	121r	93	148	41
			95	78	134	47
		70	107	33	144	45
			121r	24	133	37
		80

Positive Control	Methyl Methane Sulfonate	15	45	25	366	273	248*
Positive Control			72	18	478	222	248*

Nonactivation Trial: 5 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	71	89	158	75	80
			58	102	120	69
			75	105	198	88
			63	104	169	90
Test Chemical	Sodium dodecyl sulfate	50	56	112	155	92	70
			70	91	101	48	70
		55	74	72	99	44	43
			70	84	88	42	43
		60	82	60	127	52	48
			74	75	98	44	48
		65	67	21	124	61	60
			98	20	171	58	60
		70

Positive Control	Methyl Methane Sulfonate	15	26	24	321	412	413*
Positive Control			26	23	327	414	413*

Induced S9 Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	36r	56	60	56	30
			64	109	61	32
			70	88	53	25
			77	103	74	32
Test Chemical	Sodium dodecyl sulfate	50	37	52	64	57	45
			48	82	47	33	45
		60	40	61	44	36	42
			52	68	75	49	42
		70	21	28	35	56	41
			62	78	48	26	41
		80	10r	11	38	131
			46	42	35	25
		90	27	3	35	43	29
			58	7	26	15	29
Positive Control	3-Methylcholanthrene	2.5	16	18	430	915	699*
Positive Control			32	40	466	483	699*

Induced S9 Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	90	94	69	26	22
			87#	97	34.5	13
			104	110	84	27
Test Chemical	Sodium dodecyl sulfate	45	122r	124	25	7
			123r	102	76	21
		55	89	98	63	24
		65	92	52	65	24	27
			91	63	83	30	27
		75	89#	45	46.5	17	25
			97	41	95	33	25
		85	75#	7	36	16	22
			91	19	75	28	22
		95

Positive Control	3-Methylcholanthrene	2.5	69	44	512	249	278*
Positive Control			57	39	527	307	278*

Induced S9 Trial: 3 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	103	102	104	34	36
			90	96	62	23
			84	92	66	26
			92#	109	168	61
Test Chemical	Sodium dodecyl sulfate	60	108#	83	120	37	36
			112	94	118	35	36
		65	125r	100	98	26
			76	68	57	25
		70	94#	74	36	13
			127r	78	116	31
		75	93	62	60	22	20
			101	59	58	19	20
		80	93#	53	68	24	25
			78	55	58	25	25
Positive Control	3-Methylcholanthrene	2.5	80	43	609	255	297*
Positive Control			52	32	526	339	297*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination