Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02823-0015-0000-3
Chemical Name:	Sodium fluoride
CASRN:	7681-49-4
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	855441
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	73	111	60	27	29
			72	94	55	26
			87	104	81	31
			71	90	72	34
Test Chemical	Sodium fluoride	200	104	90	94	30	32
			67	73	67	34

		300	89	81	90	34	53*
			76	63	146	64
			91	72	164	60
		400	77	49	108	47	45*
			79	37	99	42
			80#	37	116	48
		500	76	14	117	51	55*
			68#	14	101	50
			81	22	157	65
		600	76	11	199	88	83*
			83	9	193	78	83*
		800


Positive Control	Methyl Methane Sulfonate	5	68	62	482	237	244*
			62	70	417	226
			70	80	568	270

Nonactivation Trial: 2 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	118	114	82	23	25
			88#	83	75	28
			111	103	74	22
			120r	131	92	25
Test Chemical	Sodium fluoride	50	93	90	77	28	22
			102	86	54	18
			78#	83	46	20
		100	88	63	72	27	27
			96	80	79	27	27
		200	88	61	61	23	27
			80	57	62	26
			96	53	95	33
		300	109	53	67	21	22
			75	42	41	18
			84	53	67	27
		400	116	40	137	40	36
			92	40	87	31
			131r	59	108	27
		500	97	13	166	57	41*
			106	23	123	39
			80	16	68	28
		600


Positive Control	Methyl Methane Sulfonate	5	97	68	298	102	140*
			86	59	271	105
			59	57	378	213

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	111	118	81	24	26
			108	90	104	32
			105#	89	73	23
			106	103	77	24
Test Chemical	Sodium fluoride	100	85	75	66	26
		200	114	79	40	12	21
			98	62	71	24
			87	71	68	26
		300	129r	68	86	22	39
			100#	61	105	35
			89	43	116	44
		400	111	46	109	33	39*
			116	39	105	30
			93	39	149	54
		500	90#	13	203	75	81*
			68	7	240	117
			59	20	90	51
Positive Control	3-Methylcholanthrene	2.5	51	25	534	349	335*
			67	27	629	311
			66	32	682	344

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	85	74	97	38	33
			82	118	73	30
			74	104	61	28
			88#	104	95	36
Test Chemical	Sodium fluoride	50	78	115	59	25	27
			83#	89	72	29
			73	96	60	27
		100	71	97	70	33	38
			91	117	80	29
			76	46	116	51
		200	111	96	135	40	38
			69#	78	83	40
			75	76	76	34
		300	76	48	103	45	52*
			76	56	142	62
			77	45	112	48
		400	70	27	132	63	62*
			83	28	165	66
			79	31	137	58
		500	72	17	223	103	76*
			70	19	146	69
			81	27	134	55
		600


Positive Control	3-Methylcholanthrene	2.5	46	22	777	563	513*
			44	16	703	531
			53#	25	715	447

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination