Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02823-0016-0000-4
Chemical Name:	Sodium fluoride
CASRN:	7681-49-4
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	932658
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	59	110	109	61	58
			54#	86	136.5	84
			67#	124	82	41
			55	80	76	46
Test Chemical	Sodium fluoride	62.5	58	101	162	93	105*
			47	76	162	116	105*
		125	61#	82	120	66	97*
			44	74	168	129	97*
		250	63	79	139	74	73
			55	61	121	73	73
		500	85#	35	143	56	83
			55#	37	180	109	83
		1000	36#	5	169	159	134*
			44	11	142	109	134*
Positive Control	Methyl Methane Sulfonate	15	18	15	175	318	343*
Positive Control			15	11	169	367	343*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	80	99	57	24	26
Vehicle Control			89#	101	74	28	26
Test Chemical	Sodium fluoride	600	78	56	44	19	22
			66	44	50	25	22
		700	60	40	51	29	23
			71	40	38	18	23
		800	68	34	72	35	34
			102	25	103	34	34
		900	63	20	115	61	64*
			55	14	109	66	64*
		1000	76	12	273	120	95*
			89	17	185	69	95*
Positive Control	Methyl Methane Sulfonate	15	51	40	132	86	86*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	91	113	136	50	51
			76	99	135	59
			91	101	110	40
			104	87	172	55
Test Chemical	Sodium fluoride	500	85	33	165	64	59
			79	33	125	53	59
		600	97	25	148	51	58
			77	31	149	65	58
		700	94	25	152	54	66
			87	25	202	78	66
		800	79	21	238	101	92*
			78	19	193	82	92*
		900	80	14	467	194	190*
			76	12	423	187	190*
		1000

Positive Control	Methyl Methane Sulfonate	15	56	34	91	54	49
Positive Control			48	36	63	44	49

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination