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Study Information
Study Attribute Value
CEBS Accession Number: 002-02835-0012-0000-3
Chemical Name: Sodium xylenesulfonate
CASRN: 1300-72-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 779152
Study Result: Equivocal


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 124r 92 79 21 22
    96 98 51 18
    120r 107 80 22
    103 102 79 26
Test Chemical Sodium xylenesulfonate 
125         
 66 63 60 30 26
    86 67 65 25
    93 91 64 23
  
250         
 94 77 67 24 21
    79 57 43 18
    85 69 51 20
  
500         
 99 71 62 21 21
    105 76 51 16
    87 74 71 27
  
1000         
 110 101 64 19 18
    120r 79 104 29
    100 76 48 16
  
2000         
 109 88 56 17 21
    116 94 75 22
    83 75 63 25
  
2500         
 120 78 74 21 21
    77 43 50 22
    123r 54 101 27
Positive Control Methyl Methane Sulfonate 
5         
 60 36 446 246 181*
    98 60 403 137
    79 47 381 160

Nonactivation Trial: 2 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 70 83 47 23 24
    80 121 58 24
    67 91 47 23
    79 105 62 26
Test Chemical Sodium xylenesulfonate 
250         
 62 78 45 24 23
    76 82 61 27
    89 90 50 19
  
500         
 72 75 56 26 26
    77 80 47 20
    75 74 69 31
  
750         
 91 75 46 17 24
    77 73 65 28
    80 73 66 28
  
1000         
 75 78 62 28 21
    93 90 51 18
    80 80 42 18
  
2000         
 74 68 71 32 27
    80 82 62 26
    86 81 59 23
  
2500         
 68 61 77 38 29
    79 59 45 19
    64 54 59 31
Positive Control Methyl Methane Sulfonate 
5         
 53 32 444 280 257*
    57 35 397 234
    46 55 354 257

Nonactivation Trial: 3 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 87 100 72 27 30
    79 114 72 31
    89 93 67 25
    80 93 88 37
Test Chemical Sodium xylenesulfonate 
500         
 57 22 81 48 40
    72# 64 69 32
    124r 99 75 20
  
1000         
 81 16 91 38 35
    108 62 105 32
    77 63 84 36
  
3000         
 92 74 79 29 27
    84 27 64 25
    103 41 80 26
  
4000         
 152r 40 116 26 41
    78 61 106 45
    82 65 89 36
  
5000         
 78 13 96 41 41
    65 9 93 48
    76 54 81 36
Positive Control Methyl Methane Sulfonate 
5         
 39 25 435 377 343*
    34 13 267 260
    30 5 352 391

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 79 109 94 40 34
    98 101 94 32
    80 86 80 33
    85 104 76 30
Test Chemical Sodium xylenesulfonate 
250         
 110 97 115 35 29
    78 92 52 22
    99 97 93 31
  
500         
 78 96 87 37 30
    103 99 82 27
    85 94 68 27
  
1000         
 69 110 72 35 35
    60 81 73 41
    61 87 55 30
  
2000         
 108 63 87 27 32
    109# 81 106.5 33
    76 52 85 37
  
3000         
 78 26 91 39 42
    90 24 121 45
    83 18 107 43
  
4000         
 68*
    75 10 168 75
    102 8 186 61
  
5000         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 41 15 614 499 493*
    46 8 747 545
    46 16 595 436

Induced S9 Trial: 2 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 101 138 70 23 25
    98 90 61 21
    102 69 89 29
    102 104 77 25
Test Chemical Sodium xylenesulfonate 
250         
 95 130 40 14 21
    82 60 53 22
    102 133 88 29
  
500         
 114 140 70 20 25
    98 100 106 36
    107# 153 55 17
  
1000         
 106 111 78 25 26
    104 120 82 26
    114 95 96 28
  
2000         
 104 72 86 28 33
    109 69 106 32
    95 51 107 38
Positive Control 3-Methylcholanthrene 
2.5       
 103 58 435 141 158*
    92 37 387 141
    79 15 458 194

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination