Study Attribute | Value |
---|---|
CEBS Accession Number: | 002-02835-0012-0000-3 |
Chemical Name: | Sodium xylenesulfonate |
CASRN: | 1300-72-7 |
NTP Study Type: | Genetic Toxicology - Mammalian Cell Mutagenicity |
NTP Study ID: | 779152 |
Study Result: | Equivocal |
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 124r | 92 | 79 | 21 | 22 |
96 | 98 | 51 | 18 | ||||
120r | 107 | 80 | 22 | ||||
103 | 102 | 79 | 26 | ||||
Test Chemical | Sodium xylenesulfonate | 125 | 66 | 63 | 60 | 30 | 26 |
86 | 67 | 65 | 25 | ||||
93 | 91 | 64 | 23 | ||||
250 | 94 | 77 | 67 | 24 | 21 | ||
79 | 57 | 43 | 18 | ||||
85 | 69 | 51 | 20 | ||||
500 | 99 | 71 | 62 | 21 | 21 | ||
105 | 76 | 51 | 16 | ||||
87 | 74 | 71 | 27 | ||||
1000 | 110 | 101 | 64 | 19 | 18 | ||
120r | 79 | 104 | 29 | ||||
100 | 76 | 48 | 16 | ||||
2000 | 109 | 88 | 56 | 17 | 21 | ||
116 | 94 | 75 | 22 | ||||
83 | 75 | 63 | 25 | ||||
2500 | 120 | 78 | 74 | 21 | 21 | ||
77 | 43 | 50 | 22 | ||||
123r | 54 | 101 | 27 | ||||
Positive Control | Methyl Methane Sulfonate | 5 | 60 | 36 | 446 | 246 | 181* |
98 | 60 | 403 | 137 | ||||
79 | 47 | 381 | 160 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 70 | 83 | 47 | 23 | 24 |
80 | 121 | 58 | 24 | ||||
67 | 91 | 47 | 23 | ||||
79 | 105 | 62 | 26 | ||||
Test Chemical | Sodium xylenesulfonate | 250 | 62 | 78 | 45 | 24 | 23 |
76 | 82 | 61 | 27 | ||||
89 | 90 | 50 | 19 | ||||
500 | 72 | 75 | 56 | 26 | 26 | ||
77 | 80 | 47 | 20 | ||||
75 | 74 | 69 | 31 | ||||
750 | 91 | 75 | 46 | 17 | 24 | ||
77 | 73 | 65 | 28 | ||||
80 | 73 | 66 | 28 | ||||
1000 | 75 | 78 | 62 | 28 | 21 | ||
93 | 90 | 51 | 18 | ||||
80 | 80 | 42 | 18 | ||||
2000 | 74 | 68 | 71 | 32 | 27 | ||
80 | 82 | 62 | 26 | ||||
86 | 81 | 59 | 23 | ||||
2500 | 68 | 61 | 77 | 38 | 29 | ||
79 | 59 | 45 | 19 | ||||
64 | 54 | 59 | 31 | ||||
Positive Control | Methyl Methane Sulfonate | 5 | 53 | 32 | 444 | 280 | 257* |
57 | 35 | 397 | 234 | ||||
46 | 55 | 354 | 257 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Fischer Medium | 0 | 87 | 100 | 72 | 27 | 30 |
79 | 114 | 72 | 31 | ||||
89 | 93 | 67 | 25 | ||||
80 | 93 | 88 | 37 | ||||
Test Chemical | Sodium xylenesulfonate | 500 | 57 | 22 | 81 | 48 | 40 |
72# | 64 | 69 | 32 | ||||
124r | 99 | 75 | 20 | ||||
1000 | 81 | 16 | 91 | 38 | 35 | ||
108 | 62 | 105 | 32 | ||||
77 | 63 | 84 | 36 | ||||
3000 | 92 | 74 | 79 | 29 | 27 | ||
84 | 27 | 64 | 25 | ||||
103 | 41 | 80 | 26 | ||||
4000 | 152r | 40 | 116 | 26 | 41 | ||
78 | 61 | 106 | 45 | ||||
82 | 65 | 89 | 36 | ||||
5000 | 78 | 13 | 96 | 41 | 41 | ||
65 | 9 | 93 | 48 | ||||
76 | 54 | 81 | 36 | ||||
Positive Control | Methyl Methane Sulfonate | 5 | 39 | 25 | 435 | 377 | 343* |
34 | 13 | 267 | 260 | ||||
30 | 5 | 352 | 391 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Fischer Medium | 0 | 79 | 109 | 94 | 40 | 34 |
98 | 101 | 94 | 32 | ||||
80 | 86 | 80 | 33 | ||||
85 | 104 | 76 | 30 | ||||
Test Chemical | Sodium xylenesulfonate | 250 | 110 | 97 | 115 | 35 | 29 |
78 | 92 | 52 | 22 | ||||
99 | 97 | 93 | 31 | ||||
500 | 78 | 96 | 87 | 37 | 30 | ||
103 | 99 | 82 | 27 | ||||
85 | 94 | 68 | 27 | ||||
1000 | 69 | 110 | 72 | 35 | 35 | ||
60 | 81 | 73 | 41 | ||||
61 | 87 | 55 | 30 | ||||
2000 | 108 | 63 | 87 | 27 | 32 | ||
109# | 81 | 106.5 | 33 | ||||
76 | 52 | 85 | 37 | ||||
3000 | 78 | 26 | 91 | 39 | 42 | ||
90 | 24 | 121 | 45 | ||||
83 | 18 | 107 | 43 | ||||
4000 | 68* | ||||||
75 | 10 | 168 | 75 | ||||
102 | 8 | 186 | 61 | ||||
5000 | |||||||
Positive Control | 3-Methylcholanthrene | 2.5 | 41 | 15 | 614 | 499 | 493* |
46 | 8 | 747 | 545 | ||||
46 | 16 | 595 | 436 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Fischer Medium | 0 | 101 | 138 | 70 | 23 | 25 |
98 | 90 | 61 | 21 | ||||
102 | 69 | 89 | 29 | ||||
102 | 104 | 77 | 25 | ||||
Test Chemical | Sodium xylenesulfonate | 250 | 95 | 130 | 40 | 14 | 21 |
82 | 60 | 53 | 22 | ||||
102 | 133 | 88 | 29 | ||||
500 | 114 | 140 | 70 | 20 | 25 | ||
98 | 100 | 106 | 36 | ||||
107# | 153 | 55 | 17 | ||||
1000 | 106 | 111 | 78 | 25 | 26 | ||
104 | 120 | 82 | 26 | ||||
114 | 95 | 96 | 28 | ||||
2000 | 104 | 72 | 86 | 28 | 33 | ||
109 | 69 | 106 | 32 | ||||
95 | 51 | 107 | 38 | ||||
Positive Control | 3-Methylcholanthrene | 2.5 | 103 | 58 | 435 | 141 | 158* |
92 | 37 | 387 | 141 | ||||
79 | 15 | 458 | 194 |
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination