Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02835-0012-0000-3
Chemical Name:	Sodium xylenesulfonate
CASRN:	1300-72-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	779152
Study Result:	Equivocal

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	124r	92	79	21	22
			96	98	51	18
			120r	107	80	22
			103	102	79	26
Test Chemical	Sodium xylenesulfonate	125	66	63	60	30	26
			86	67	65	25
			93	91	64	23
		250	94	77	67	24	21
			79	57	43	18
			85	69	51	20
		500	99	71	62	21	21
			105	76	51	16
			87	74	71	27
		1000	110	101	64	19	18
			120r	79	104	29
			100	76	48	16
		2000	109	88	56	17	21
			116	94	75	22
			83	75	63	25
		2500	120	78	74	21	21
			77	43	50	22
			123r	54	101	27
Positive Control	Methyl Methane Sulfonate	5	60	36	446	246	181*
			98	60	403	137
			79	47	381	160

Nonactivation Trial: 2 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	70	83	47	23	24
			80	121	58	24
			67	91	47	23
			79	105	62	26
Test Chemical	Sodium xylenesulfonate	250	62	78	45	24	23
			76	82	61	27
			89	90	50	19
		500	72	75	56	26	26
			77	80	47	20
			75	74	69	31
		750	91	75	46	17	24
			77	73	65	28
			80	73	66	28
		1000	75	78	62	28	21
			93	90	51	18
			80	80	42	18
		2000	74	68	71	32	27
			80	82	62	26
			86	81	59	23
		2500	68	61	77	38	29
			79	59	45	19
			64	54	59	31
Positive Control	Methyl Methane Sulfonate	5	53	32	444	280	257*
			57	35	397	234
			46	55	354	257

Nonactivation Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	87	100	72	27	30
			79	114	72	31
			89	93	67	25
			80	93	88	37
Test Chemical	Sodium xylenesulfonate	500	57	22	81	48	40
			72#	64	69	32
			124r	99	75	20
		1000	81	16	91	38	35
			108	62	105	32
			77	63	84	36
		3000	92	74	79	29	27
			84	27	64	25
			103	41	80	26
		4000	152r	40	116	26	41
			78	61	106	45
			82	65	89	36
		5000	78	13	96	41	41
			65	9	93	48
			76	54	81	36
Positive Control	Methyl Methane Sulfonate	5	39	25	435	377	343*
			34	13	267	260
			30	5	352	391

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	79	109	94	40	34
			98	101	94	32
			80	86	80	33
			85	104	76	30
Test Chemical	Sodium xylenesulfonate	250	110	97	115	35	29
			78	92	52	22
			99	97	93	31
		500	78	96	87	37	30
			103	99	82	27
			85	94	68	27
		1000	69	110	72	35	35
			60	81	73	41
			61	87	55	30
		2000	108	63	87	27	32
			109#	81	106.5	33
			76	52	85	37
		3000	78	26	91	39	42
			90	24	121	45
			83	18	107	43
		4000					68*
			75	10	168	75
			102	8	186	61
		5000


Positive Control	3-Methylcholanthrene	2.5	41	15	614	499	493*
			46	8	747	545
			46	16	595	436

Induced S9 Trial: 2 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	101	138	70	23	25
			98	90	61	21
			102	69	89	29
			102	104	77	25
Test Chemical	Sodium xylenesulfonate	250	95	130	40	14	21
			82	60	53	22
			102	133	88	29
		500	114	140	70	20	25
			98	100	106	36
			107#	153	55	17
		1000	106	111	78	25	26
			104	120	82	26
			114	95	96	28
		2000	104	72	86	28	33
			109	69	106	32
			95	51	107	38
Positive Control	3-Methylcholanthrene	2.5	103	58	435	141	158*
			92	37	387	141
			79	15	458	194

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination