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Study Information
Study Attribute Value
CEBS Accession Number: 002-02837-0010-0000-3
Chemical Name: Stannous chloride
CASRN: 7772-99-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 671517
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 104 99 48 15 21
    105 88 72 23
    90 91 56 21
    95 123 75 26
Test Chemical Stannous chloride 
10         
 88 69 56 21 26
    89 57 64 24
    104# 77 102 33
  
20         
 92 62 84 31 30
    89 53 69 26
    87 52 90 35
  
30         
 88 50 111 42 32
    79 54 66 28
    73 46 58 27
  
40         
 86 45 82 32 38*
    89 35 106 40
    111 26 136 41
  
50         
 104 47 91 29 34*
    79 47 80 34
    97 31 110 38
  
60         
 102 37 99 32 31
    88 38 80 30
    122r 45 120 33
Positive Control Methyl Methane Sulfonate 
5         
 39 31 343 293 266*
    93 50 580 207
    65 35 575 296

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 92 81 84 30 30
    107 124 104 32
    112 93 112 33
    112 101 85 25
Test Chemical Stannous chloride 
20         
 100 68 92 31 31
    111 68 117 35
    107 74 87 27
  
30         
 95 77 124 43 33
    92 64 87 32
    113 60 81 24
  
40         
 112 66 101 30 28
    115 69 96 28
    114 46 85 25
  
50         
 87 73 80 31 30
    109 71 87 27
    118 70 112 32
  
60         
 96 46 107 37 37
    100 44 96 32
    101# 42 130 43
  
80         
 83 35 132 53 52*
    108 50 206 63
    110 50 130 39
Positive Control Methyl Methane Sulfonate 
5         
 57# 34 405 235 237*
    70# 57 442 212
    69 44 543 264

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 112 98 148 44 43
    105 97 143 45
    110 100 147 44
    110 105 129 39
Test Chemical Stannous chloride 
20         
 96 53 114 40 41
    86 39 107 41
    74 41 94 42
  
30         
 80 32 149 62 54
    87 42 148 56
    103 16 130 42
  
40         
 109 65 130 40 39
    90 24 105 39
    98 51 115 39
  
50         
 80 35 106 44 50
    103 33 119 38
    66 22 136 69
  
60         
 108 37 141 44 39
    95 45 112 39
    114 76 112 33
  
80         
 92 41 152 55 53
    83 15 149 60
    96 49 125 44
Positive Control Methyl Methane Sulfonate 
5         
 61 35 807 443 396*
    73 61 578 263
    66 48 948 481

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 107 114 66 21 23
    74 82 64 29
    88 112 60 23
    83 92 51 21
Test Chemical Stannous chloride 
30         
 96 70 82 29 28
    125r 80 122 33
    107 104 87 27
  
40         
 100 79 72 24 28
    89 66 96 36
    99 68 72 24
  
50         
 104 80 86 27 35*
    101 56 120 39
    79 52 91 38
  
60         
 116 65 122 35 32
    113 72 72 21
    102 55 124 41
  
80         
 103r 55 86 28
    132r 63 136 34
    136r 64 117 29
  
100         
 97 49 110 38 30
    127r 54 124 32
    111 38 72 22
Positive Control 3-Methylcholanthrene 
2.5       
 109 78 383 117 150*
    96 73 459 160
    84 53 435 173

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 118 102 151 43 46
    115 103 176 51
    106 95 142 45
Test Chemical Stannous chloride 
30         
 112 91 116 34 42
    71 52 122 58
    84 79 84 33
  
40         
 82 67 90 37 41
    82 69 113 46
    126r 101 105 28
  
50         
 97 63 111 38 38
    112 63 124 37
    120r 73 107 30
  
60         
 125r 67 158 42
    129r 73 199 51
    102 71 151 50
  
80         
 112 62 144 43 48
    115 62 184 53
    120 65 171 48
  
100         
 117 63 152 43 42
    121r 61 150 41
    118 69 143 40
Positive Control 3-Methylcholanthrene 
2.5       
 61 21 360 197 329*
    55 10 453 275
    46 14 711 515

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination