Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02849-0001-0000-6
Chemical Name:	Sucrose
CASRN:	57-50-1
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	330941
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	68	97	108	53	47
			50	73	78	52
			68	129	82	40
			65	101	81	42
Test Chemical	Sucrose	156.25	50	64	98	66	59
			57	80	90	53	59
		312.5	36	36	72	67	63
			63	80	110	58	63
		625	39	34	55	47	50
			65	73	101	52	50
		1250	27	33	27	34	43
			64	67	99	52	43
		2500	27	30	44	54	49
			73	83	96	44	49
		5000	23	23	56	81	67
			73	74	115	52	67
Positive Control	Ethyl Methane Sulfonate	200	67	69	345	171	166*
Positive Control			65	74	313	160	166*

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	90#	99	103.5	39	35
			93	101	90	32
			136r	191	144	35
Test Chemical	Sucrose	312.5	132r	160	108	27
			94	104	72	25
		625	96#	104	87	30
			127r	149	127	33
		1250	93	97	56	20	29
			118#	137	138	39	29
		2500	80	85	49	21
		5000	89	110	83	31
			126r	134	117	31
Positive Control	Ethyl Methane Sulfonate	250	64	60	301	157	157*
Positive Control			130r	196	441	113	157*

Non-Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	80	93	66	28	38
			79	105	116	49
			71	106	98	46
			90	96	75	28
Test Chemical	Sucrose	312.5	70	116	92	44	48
			77	93	120	52	48
		625	88	109	105	40	40
			66	86	79	40	40
		1250	67	92	88	44	43
			75	73	92	41	43
		2500	68	84	76	37	39
			67	92	82	41	39
		5000	62	75	114	62	45
			86	93	72	28	45
Positive Control	3-Methylcholanthrene	2.5	78	94	250	107	109*
Positive Control			82	92	275	112	109*

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	61	92	190	104	100
			55	97	193	118
			51	112	169	111
			68	98	137	67
Test Chemical	Sucrose	312.5	75	127	152	68	86
			52#	90	160.5	104	86
		625	59	86	239	135	110
			64	83	164	86	110
		1250	71	104	136	64	86
			58	67	186	107	86
		2500	56	104	169	101	99
			71	101	205	96	99
		5000	62	121	205	111	99
			59	120	154	88	99
Positive Control	3-Methylcholanthrene	2.5	33	40	471	471	373*
Positive Control			64	81	525	275	373*

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	78	95	162	69	68
			76	100	147	64
			69	87	162	78
			88	119	165	63
Test Chemical	Sucrose	312.5	74	116	172	77	74
			70	111	149	71	74
		625	76	88	218	96	79
			69	79	127	61	79
		1250	88	112	171	65	75
			71	91	182	85	75
		2500	84	107	162	64	71
			62	113	144	77	71
		5000	87	129	169	65	70
			66	76	149	76	70
Positive Control	3-Methylcholanthrene	2.5	50	37	838	557	812*
Positive Control			27	17	853	1066	812*

Induced S9 Trial: 3 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	115	103	213	62	63
			113	98	228	67
			111	112	182	55
			94	88	198	70
Test Chemical	Sucrose	312.5	83	91	198	80	81
			109	93	267	82	81
		625	85	91	241	95	88
			86	102	208	80	88
		1250	68	89	205	100	98
			82	89	232	95	98
		2500	83	103	212	85	87
			65	84	175	89	87
		5000	91	88	210	77	80
			79	84	195	83	80
Positive Control	3-Methylcholanthrene	2.5	70	40	711	341	312*
Positive Control			81	44	690	284	312*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination