Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02849-0002-0000-7
Chemical Name:	Sucrose
CASRN:	57-50-1
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	341303
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	88	100	163	62	63
			81	92	185	77
			85	105	117	46
			86#	103	174	67
Test Chemical	Sucrose	1311	90	108	181	67	78
			78	97	202	87
			80	100	191	80
		1638	82	106	162	66	82
			76	100	213	94
			80	95	207	86
		2048	79	87	172	73	73
			78	97	165	71
			72	76	165	77
		2560	75	93	137	61	77
			69	83	160	77
			81	93	229	94
		3200	82	94	199	81	77
			87	92	203	78
			70	77	154	73
		4000	86	107	199	77	68
			87	113	143	55
			88	105	190	72
		5000	74	84	204	92	75
			86	103	160	62
			72	89	151	70
Positive Control	Ethyl Methane Sulfonate	500	44	31	994	756	767*
			47	35	975	694
			38	27	965	850

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	104	104	49	16	18
			89	112	55	21
			98	118	48	16
			68	66	36	18
Test Chemical	Sucrose	1311	83	119	33	13	30*
			48	74	65	45
			66#	112	62	31
		1638	67	99	41	20	20
			83	136	63	25
			85	137	38	15
		2048	51	83	53	34	29*
			62	109	47	25
			60	96	50	28
		2560	78	132	53	23	21
			64	102	29	15
			98	133	72	25
		3200	84	131	45	18	19
			87	117	25	10
			81	114	69	28
		4000	84#	115	79.5	32	23
			94	106	42	15	23
		5000	75	115	47	21	19
			89	109	47	18	19
Positive Control	Ethyl Methane Sulfonate	500	53#	48	1012.5	637	673*
Positive Control			46	35	985	709	673*

Induced S9 Trial: 1 Experiment Call: No Call
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	88	109	377	143	158
			80	93	396	166
			79	106	369	156
			77	92	385	166
Test Chemical	Sucrose	1638	82	108	355	144	134
			89	124	333	124	134
		2048	91	107	397	145	238
			46	47	456	330	238
		2560	90	110	383	142	137
			89	110	351	131	137
		3200	94	105	363	129	140
			86	94	389	151	140
		4000	70	94	357	170	160
			81	99	362	150	160
		5000	74	88	310	140	147
			80	88	371	155	147
Positive Control	3-Methylcholanthrene	5	10r	2	155	534	356*
			14	2	146	356
			16	2	173	357

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	68	76	28	14	18
			83	127	64	26
			66	97	29	15
Test Chemical	Sucrose	1311	68	107	34	17	12
			68#	108	16.5	8	12
		1638	64#	100	45	23	22
			57#	89	44	26
			67	106	33	16
		2048	89#	130	43	16	15
			67	108	42	21
			71	110	18	8
		2560	228r	330	180	26	17
			65	89	40	20
			50	72	19	13
		3200	75#	94	27	12	21
			69	91	35	17
			79	100	82	35
		4000	60	92	38	21	17
			66	95	26	13
			65	97	30	15
		5000	65	90	26	13	19
			71	91	33	15
			70	74	57	27
Positive Control	3-Methylcholanthrene	6	47	42	374	266	292*
			35	33	340	327
			47	40	396	284

Induced S9 Trial: 3 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	90	98	197	73	79
			91	99	202	74
			90	103	244	91
Test Chemical	Sucrose	1311	86	90	186	72	69
			84	92	197	78
			91	94	154	57
		1638	92	100	215	78	73
			76	83	157	69
			90	104	191	71
		2048	95	104	181	64	72
			91	105	205	75
			77	84	180	78
		2560	86	94	256	99	85
			85	93	183	72
			87	87	220	84
		3200	84	92	192	76	71
			88	101	176	67
			108	118	224	69
		4000	83	86	202	81	77
			86	93	203	79
			87	94	188	72
		5000	88	91	144	55	62
			80	87	167	70
			82	89	152	62
Positive Control	3-Methylcholanthrene	5	64#	49	861	447	424*
			57	45	788	465
			74	57	801	362

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination