Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02849-0003-0000-8
Chemical Name:	Sucrose
CASRN:	57-50-1
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	774043
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	63	79	25	13	17
			80	113	39	16
			81	134	28	12
			54	75	47	29
Test Chemical	Sucrose	156	93	140	35	13	13
			97	121	41	14	13
		313	104	97	58	19	17
			88	123	39	15	17
		625	71	90	38	18	17
			78	126	38	16	17
		1250	62	81	31	17	19
			73	85	49	22	19
		2500	72	95	19	9	13
			68	96	36	18	13
		5000	65	93	24	12	15
			55	76	30	18	15
Positive Control	Ethyl Methane Sulfonate	500	38	18	886	784	795*
			37	22	977	872
			43	25	939	728

Nonactivation Trial: 2 Experiment Call: No Call
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	88	103	195	74	72
			73	104	133	61
			78	100	185	79
			101	94	227	75
Test Chemical	Sucrose	500	97	81	247	85	81
			98	98	223	76
			78	87	188	81
		1000	101	107	231	76	86
			79	85	219	92
			90	88	238	88
		2000	68	97	172	84	85
			105	111	233	74
			75	101	219	98
		4000	74	89	185	84	76
			98	104	220	75
			76	110	155	68
		5000	92	100	215	78	79
			87	108	198	76
			82	97	206	83
Positive Control	Ethyl Methane Sulfonate	500	4r	1	169	1470
			2r	0	84	1400
			5r	1	203	1353

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	86	91	58	22	18
			90	114	43	16
			92	89	45	16
			91	106	50	18
Test Chemical	Sucrose	500	86	91	38	15	19
			86	87	48	19
			81	86	55	23
		1000	83	88	60	24	21
			67	80	41	20
			83#	95	46	18
		2000	77	77	64	28	23
			76	94	45	20
			81	91	55	23
		4000	92#	95	40.5	15	22
			84#	116	63	25
			91	90	68	25
		5000	93	85	65	23	22
			93	96	49	18
			88	72	68	26
Positive Control	Ethyl Methane Sulfonate	500	33	12	931	955	809*
			35	19	750	714
			38#	17	873	759

Non-Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	81	107	68	28	28
			96	127	63	22
			64#	86	57	30
			63	80	65	34
Test Chemical	Sucrose	500	95	177	59	21	23
			81	107	58	24
			99	133	69	23
		1000	77	129	55	24	22
			87	118	45	17
			77	129	55	24
		2000	64	103	51	27	29
			77	90	60	26
			81	122	82	34
		4000	65	80	38	20	23
			77	91	49	21
			80#	86	71	30
		5000	62	89	65	35	32
			60	73	60	34
			58#	95	46	26
Positive Control	3-Methylcholanthrene	7	59	47	358	201	160*
			65	55	275	141
			67	62	278	138

Non-Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	98	119	89	30	27
			92	87	66	24
			99	94	83	28
Test Chemical	Sucrose	500	66	87	51	26	29
			104	97	101	32
			91	95	78	29
		1000	99	77	90	30	36
			87	90	105	40
			88	76	95	36
		2000	91	94	106	39	39
			94	66	124	44
			96	67	98	34
		4000	102	77	121	40	34
			90	82	76	28
			97	81	97	33
		5000	98	86	108	37	37
			96	91	106	37	37
Positive Control	3-Methylcholanthrene	7	80	53	510	213	192*
			81	49	454	187
			79	54	418	176

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	87	86	56	21	24
			101	123	75	25
			76	101	53	23
			97	90	82	28
Test Chemical	Sucrose	156	84	93	65	26	24
			81	98	52	21	24
		313	103	93	82	27	29
			90	89	85	31	29
		625	82	109	72	29	24
			81	88	47	19	24
		1250	83	90	53	21	19
			77	100	37	16	19
		2500	90	98	68	25	27
			95	92	84	30	27
		5000	79	84	72	30	31
			76	92	72	32	31
Positive Control	3-Methylcholanthrene	5	41	10	399	328	369*
			27	7	341	416
			37	9	404	364

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	63#	67	49.5	26	25
			88	111	83	31
			82	111	53	22
			98	111	62	21
Test Chemical	Sucrose	500	60	92	40	22	19
			75	108	50	22
			77	118	32	14
		1000	74#	87	31.5	14	22
			75	89	63	28
			76	104	57	25
		2000	101	134	59	19	19
			73	96	30	14
			68	68	47	23
		4000	83	82	51	20	22
			92	94	61	22
			101	110	67	22
		5000	87	111	62	24	20
			91	113	54	20
			85	114	39	15
Positive Control	3-Methylcholanthrene	5	49#	21	345	235	287*
			38#	15	343	303
			25#	10	238.5	324

Induced S9 Trial: 3 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	93#	93	43.5	16	15
			102	109	43	14
			78	98	35	15
Test Chemical	Sucrose	500	80	82	31	13	16
			85	94	52	20
			89	93	41	15
		1000	75	87	35	15	18
			81	76	47	19
			90#	84	49.5	18
		2000	96	89	47	16	18
			94	75	57	20
			97	97	50	17
		4000	98	71	67	23	21
			101	89	59	19
			92#	69	58	21
		5000	92#	87	49.5	18	18
			104	95	41	13
			97	76	67	23
Positive Control	3-Methylcholanthrene	5	65	29	378	193	216*
			59#	27	450	253
			57#	24	349.5	203

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination