Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02859-0010-0000-7
Chemical Name:	Sulfisoxazole
CASRN:	127-69-5
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	112199
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	112	116	67	20	28
			107	89	71	22
			108	99	118	36
			83	97	87	35
Test Chemical	Sulfisoxazole	62.5	97	103	84	29	25
			113	102	75	22
			106	107	76	24
		125	111	90	94	28	30
			95	68	89	31
			116	88	108	31
		250	105	84	90	29	29
			111	72	107	32
			111	104	83	25
		500	102	80	116	38	36
			85	37	108	43
			114	75	98	29
		750	63	22	115	61	43*
			105#	70	86	27
			92	39	115	42
		1000	78	12	290	125	82*
			78	25	116	50
			74	13	159	72
Positive Control	Methyl Methane Sulfonate	5	94	79	226	80	77*
			84	83	217	86
			75	90	142	63

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	90	102	143	53	54
			100	97	186	62
			121r	118	175	48
			98	101	134	46
Test Chemical	Sulfisoxazole	100	126r	99	183	48
			120#	119	199	56
			124r	130	176	47
		200	122r	85	186	51
			126r	80	183	48
			128r	104	180	47
		400	69	17	192	93
			126r	54	186	49
			121r	66	280	77
		600	132r	40	116	29	67
			115	22	221	64
			114	69	242	71
Positive Control	Methyl Methane Sulfonate	5	35	16	791	757	674*
			61	56	1036	563
			58	52	1220	701

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination