Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02876-0014-0000-0
Chemical Name:	Tetrachloroethylene
CASRN:	127-18-4
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	616544
Study Result:	Equivocal

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	103	111	53	17	23
			72	100	70	32
			86	87	58	23
			82	101	54	22
Test Chemical	Tetrachloroethylene	6.25	89	78	98	37	42*
			82#	87	108	44
			71	80	95	45
		12.5	67	109	68	34	35
			58#	91	70	40
			75	102	71	31
		25	88	123	123	47	44*
			69	114	92	44
			65	120	82	42
		50	78#	87	126	54	50*
			68#	72	109	53
			91	107	115	42
		100	83	95	132	53	45*
			69	78	85	41
			81	91	98	40
		200	86	59	120	47


		300


Positive Control	Methyl Methane Sulfonate	5	71#	52	303	143	122*
			76	51	308	135
			78	80	201	86

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	92	95	133	48	52
			81	116	126	52
			88#	95	141	53
			79	94	130	55
Test Chemical	Tetrachloroethylene	6.25	81	117	100	41	36
			74#	111	83	37
			89#	102	80	30
		12.5	89	141	102	38	63
			103	105	169	55
			81#	120	232	95
		25	91	112	105	39	37
			76	73	99	44
			112#	120	93	28
		50	76	129	86	38	39
			70#	102	86	41
			92#	92	108	39
		100	82	33	112	46	46
			67	85	104	52
			89	74	112	42
		150	70	20	108	51	73
			77#	80	112	49
			72	6	257	120
		200


Positive Control	Methyl Methane Sulfonate	5	93#	99	462	165	211*
			79	89	555	234
			70	68	491	235

Nonactivation Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	92	107	89	32	24
			66	95	36	18
			67	98	45	22
Test Chemical	Tetrachloroethylene	25	54	68	67	41	28
			97	118	66	23
			82	106	53	22
		50	95	120	78	27	26
			83	114	78	32
			76	88	45	20
		100	70	10	68	32	25
			74	107	49	22
			104	97	66	21
		125					25
			66	71	55	28
			58	72	37	21
		150


Positive Control	Methyl Methane Sulfonate	5	24	11	523	716	657*
			33	36	777	777
			59	39	848	478

Nonactivation Trial: 4 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	132r	108	242	61	58
			114#	100	200	58
			125r	115	223	59
			114	100	200	58
Test Chemical	Tetrachloroethylene	25	118	113	186	53	51
			113	105	172	51
			105	72	159	50
		50	114	70	183	54	50
			113	68	182	54
			111	92	139	42
		75	113	60	232	68	56
			117	67	172	49
			103	90	160	52
		100	122r	48	187	51	49
			120	88	177	49
			116	71	168	48
		150	116#	69	165	47	48
			116	27	151	43
			111	67	179	54
		175	118	71	169	48	52
			95	8	161	56

		200


Positive Control	Methyl Methane Sulfonate	5	60	33	310	173	190*
			66	40	327	164
			67	58	465	231

Non-Induced S9 Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	105	142	223	71	79
			71	79	150	71
			71	81	208	97
			78	98	184	79
Test Chemical	Tetrachloroethylene	25	57	78	79	47	38
			68	120	79	39
			78	142	65	28
		50	80	72	161	67	52
			75	84	103	46
			57	95	73	43
		75	83	48	159	64	66
			72	82	154	71
			74	96	138	62
		100	74	67	187	84	68
			84	90	130	52
			98	21	201	69
		150					96
			84	41	167	67
			68	8	255	126
		200


Positive Control	3-Methylcholanthrene	3.5	83	47	976	394	386*
			67	49	778	385
			75	60	851	379

Induced S9 Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	114	103	261	76	84
			81	87	186	77
			114	105	279	82
			88	104	270	103
Test Chemical	Tetrachloroethylene	12.5	71	86	130	61	109
			85	86	324	127
			77	74	326	141
		25	81	71	284	118	122*
			73	71	243	111
			84	60	342	136
		50	74	84	195	88	105
			77	56	290	126
			87	87	261	100
		100	84	75	200	79	94
			76	55	273	121
			64	54	161	83
		150	87	88	135	52	64
			98	59	193	66
			81	45	183	75
		200	109	66	263	81	73
			87	49	236	91
			91	29	127	47
Positive Control	3-Methylcholanthrene	2.5	105	72	549	175	230*
			80	33	648	272
			79	78	574	244

Induced S9 Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	89	121	211	79	75
			98	116	200	68
			67#	93	162	80
			60	70	134	74
Test Chemical	Tetrachloroethylene	50	80	92	226	94	95
			74	83	220	99
			70	83	189	90
		75	76	88	195	86	90
			85	104	217	85
			64	90	192	101
		100	87	93	212	82	99
			107	78	330	103
			78	67	263	112
		150	73	55	190	87	83
			85	70	221	87
			73	83	165	76
		200	86	60	96	37	72
			105	85	319	101
			91	72	209	77
		250	87	37	214	82	85

			77	30	204	88
		300


Positive Control	3-Methylcholanthrene	2.5	56	34	743	441	545*
			35	6	712	681
			46	18	705	513

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination