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Study Information
Study Attribute Value
CEBS Accession Number: 002-02876-0015-0000-1
Chemical Name: Tetrachloroethylene
CASRN: 127-18-4
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 642016
Study Result: Equivocal


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 80 103 49 20 25
    122r 75 92 25
    89# 103 91.5 34
    88 94 49 19
Test Chemical Tetrachloroethylene 
12.5       
 87 105 100 38
  
25         
 87 110 60 23 29
    101 102 109 36
  
50         
 78# 113 78 34 32
    99 113 88 30
  
100         
 104# 90 104 33 29
    93# 113 68 24
  
200         
 68 57 72 35 39
    93# 62 117 42
Positive Control Methyl Methane Sulfonate 
15         
 24 19 124 173 173*
    24 19 121 172

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 98# 111 150 51 59
    86 85 155 60
    107# 104 213 66
Test Chemical Tetrachloroethylene 
50         
 97 93 159 55 60
    79 97 154 65
  
100         
 91 64 85 31 34
    111# 74 121.5 37
  
150         
 72 6 512 239 357*
    62 5 888 476
  
200         
   
Positive Control Methyl Methane Sulfonate 
15         
 43# 35 583.5 449 468*
    34# 26 502.5 488

Nonactivation Trial: 3 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 85 92 178 70 60
    81 98 108 44
    62 105 112 60
    95 105 187 66
Test Chemical Tetrachloroethylene 
125         
 98 93 119 40 49
    95 89 165 58
  
150         
 75 102 142 63 70
    69 76 159 77
  
175         
 92 94 174 63 63
    85 81 159 63
  
200         
 82 94 176 72 66
    70 87 125 60
  
225         
 87 89 209 80 76
    93 102 202 73
Positive Control Methyl Methane Sulfonate 
15         
 39 30 246 210 210*
    49 38 306 209

Nonactivation Trial: 4 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 86 103 153 59 69
    73# 98 156 71
    80 105 148 62
    65 93 165 85
Test Chemical Tetrachloroethylene 
50         
 99 104 212 71 66
    74 107 136 61
  
100         
 91 50 205 75 73
    90 60 194 72
  
150         
 108 67 163 51 55
    107 30 191 60
  
200         
   
Positive Control Methyl Methane Sulfonate 
15         
 54 32 338 207 183*
    58 40 274 159

Nonactivation Trial: 5 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 65 92 65 33 33
    69 78 53 26
    76 103 80 35
    86 127 94 37
Test Chemical Tetrachloroethylene 
125         
 75 41 84 37 35
    59 33 56 32
  
150         
 59 29 45 25 41
    70 29 118 57
  
175         
 58 9 207 119 115*
    59 14 198 112
  
200         
   
Positive Control Methyl Methane Sulfonate 
15         
 27 27 137 168 153*
    30 25 122 137
Methyl Methane Sulfonate 
250         
 65 82 290 148 153*
    68 99 322 159

Induced S9 Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 75 122 130 58 52
    66 94 97 49
    77 103 99 43
    80 81 139 58
Test Chemical Tetrachloroethylene 
25         
 81 87 119 49 49
    79 82 115 48
  
50         
 82 87 101 41 50
    76 84 135 60
  
100         
 117 57 190 54 53
    98 59 150 51
  
150         
 74 52 130 59 56
    77 59 125 54
  
200         
   
Positive Control 3-Methylcholanthrene 
2.5       
 63 50 433 231 240*
    57 42 430 250

Induced S9 Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 70 102 90 43 50
    93 101 137 49
    77 95 145 63
    78 102 107 46
Test Chemical Tetrachloroethylene 
25         
 68 90 124 61 48
    88 89 95 36
  
75         
 69 79 112 54 50
    81 89 114 47
  
125         
 66 77 76 39 33
    82 88 69 28
  
175         
 73 33 121 55 50
    78 47 102 44
  
225         
   
Positive Control 3-Methylcholanthrene 
2.5       
 65 59 371 189 191*
    77 59 449 194

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination