Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02876-0015-0000-1
Chemical Name:	Tetrachloroethylene
CASRN:	127-18-4
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	642016
Study Result:	Equivocal

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	80	103	49	20	25
			122r	75	92	25
			89#	103	91.5	34
			88	94	49	19
Test Chemical	Tetrachloroethylene	12.5	87	105	100	38
		25	87	110	60	23	29
			101	102	109	36	29
		50	78#	113	78	34	32
			99	113	88	30	32
		100	104#	90	104	33	29
			93#	113	68	24	29
		200	68	57	72	35	39
			93#	62	117	42	39
Positive Control	Methyl Methane Sulfonate	15	24	19	124	173	173*
Positive Control			24	19	121	172	173*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	98#	111	150	51	59
			86	85	155	60
			107#	104	213	66
Test Chemical	Tetrachloroethylene	50	97	93	159	55	60
			79	97	154	65	60
		100	91	64	85	31	34
			111#	74	121.5	37	34
		150	72	6	512	239	357*
			62	5	888	476	357*
		200

Positive Control	Methyl Methane Sulfonate	15	43#	35	583.5	449	468*
Positive Control			34#	26	502.5	488	468*

Nonactivation Trial: 3 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	85	92	178	70	60
			81	98	108	44
			62	105	112	60
			95	105	187	66
Test Chemical	Tetrachloroethylene	125	98	93	119	40	49
			95	89	165	58	49
		150	75	102	142	63	70
			69	76	159	77	70
		175	92	94	174	63	63
			85	81	159	63	63
		200	82	94	176	72	66
			70	87	125	60	66
		225	87	89	209	80	76
			93	102	202	73	76
Positive Control	Methyl Methane Sulfonate	15	39	30	246	210	210*
Positive Control			49	38	306	209	210*

Nonactivation Trial: 4 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	86	103	153	59	69
			73#	98	156	71
			80	105	148	62
			65	93	165	85
Test Chemical	Tetrachloroethylene	50	99	104	212	71	66
			74	107	136	61	66
		100	91	50	205	75	73
			90	60	194	72	73
		150	108	67	163	51	55
			107	30	191	60	55
		200

Positive Control	Methyl Methane Sulfonate	15	54	32	338	207	183*
Positive Control			58	40	274	159	183*

Nonactivation Trial: 5 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	65	92	65	33	33
			69	78	53	26
			76	103	80	35
			86	127	94	37
Test Chemical	Tetrachloroethylene	125	75	41	84	37	35
			59	33	56	32	35
		150	59	29	45	25	41
			70	29	118	57	41
		175	58	9	207	119	115*
			59	14	198	112	115*
		200

Positive Control	Methyl Methane Sulfonate	15	27	27	137	168	153*
			30	25	122	137	153*
	Methyl Methane Sulfonate	250	65	82	290	148	153*
			68	99	322	159	153*

Induced S9 Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	75	122	130	58	52
			66	94	97	49
			77	103	99	43
			80	81	139	58
Test Chemical	Tetrachloroethylene	25	81	87	119	49	49
			79	82	115	48	49
		50	82	87	101	41	50
			76	84	135	60	50
		100	117	57	190	54	53
			98	59	150	51	53
		150	74	52	130	59	56
			77	59	125	54	56
		200

Positive Control	3-Methylcholanthrene	2.5	63	50	433	231	240*
Positive Control			57	42	430	250	240*

Induced S9 Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	70	102	90	43	50
			93	101	137	49
			77	95	145	63
			78	102	107	46
Test Chemical	Tetrachloroethylene	25	68	90	124	61	48
			88	89	95	36	48
		75	69	79	112	54	50
			81	89	114	47	50
		125	66	77	76	39	33
			82	88	69	28	33
		175	73	33	121	55	50
			78	47	102	44	50
		225

Positive Control	3-Methylcholanthrene	2.5	65	59	371	189	191*
Positive Control			77	59	449	194	191*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination