Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02876-0016-0000-2
Chemical Name:	Tetrachloroethylene
CASRN:	127-18-4
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	776521
Study Result:	Equivocal

View Study Details | View Protocol | Download To Excel

Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	65	101	139	71	65
			54#	87	111	69
			58	110	106	61
			69#	102	119	58
Test Chemical	Tetrachloroethylene	3.13	70	91	98	47	46
			71	109	96	45
			70	132	97	46
		6.25	71	100	124	58	51
			63	117	94	50
			56#	110	77	46
		12.5	66	109	89	45	44
			71	143	103	49
			105	151	119	38
		25	54	91	70	44	41
			70	101	98	47
			91	145	86	31
		50	70	66	95	45	52
			69	75	99	48
			59	53	111	63
		100


Positive Control	Ethyl Methane Sulfonate	250	50	54	892	599	727*
			33	38	822	826
			34	37	761	757

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	102	72	74	24	24
			101	117	78	26
			93	111	61	22
Test Chemical	Tetrachloroethylene	12.5	72	62	80	37	33
			71	72	69	32
			58	71	50	29
		25	96	64	91	32	31
			104	65	94	30
			79	57	76	32
		50	63	36	71	37	30
			97	64	76	26
			71	45	54	25
		75	75	31	82	37	33
			54	30	50	31
			71	39	64	30
		100	67	21	63	31	50*
			47#	5	96	69	50*
		150	75	25	95	42	34
			76	32	79	35
			84	38	66	26
Positive Control	Ethyl Methane Sulfonate	250	96	92	947	328	402*
			82#	55	1158	471
			80	76	976	408

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	72	96	63	29	25
			74#	62	41	18
			87	142	69	27
Test Chemical	Tetrachloroethylene	12.5	83	134	82	33	27
			75	127	51	23
			65	93	51	26
		25	67	80	64	32	26
			68	102	49	24
			95	109	60	21
		50	72	33	77	35	30
			84#	64	77	31
			92	92	64	23
		75
			82	75	60	24

		100


Positive Control	Methyl Methane Sulfonate	10	39	24	427	362	390*
			29	19	365	417	390*
	Methyl Methane Sulfonate	250	88#	89	610	231	231*

Non-Induced S9 Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	58#	101	72	41	35
			62	97	63	34
			73	101	62	28
Test Chemical	Tetrachloroethylene	12.5	64	90	60	31	27
			77#	84	63	27
			85#	150	56	22
		25	50#	71	77	52	43
			79	101	83	35	43
		50	73	91	74	34	31
			104	103	87	28	31
		75	87#	94	85	33	41
			71	47	106	50	41
		100	76	74	81	36
		150


Positive Control	3-Methylcholanthrene	3.5	58	57	364	209	186*
Positive Control			62	64	304	164	186*

Induced S9 Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	98	107	82	28	28
			97	99	79	27
			103	97	95	31
			99	97	72	24
Test Chemical	Tetrachloroethylene	3.13	83	71	62	25	28
			89	98	86	32
			91	95	76	28
		6.25	83	85	62	25	27
			91#	40	75	27
			87	56	74	28
		12.5	102	95	72	23	25
			94	89	59	21
			79	71	75	32
		25	92#	123	82.5	30	27
			95	88	86	30
			99#	79	63	21
		50	105	89	83	26	22
			90	54	48	18
			120r	72	71	20
		100	86	51	68	27	24
			113#	38	75	22

		200


Positive Control	3-Methylcholanthrene	2.5	97	51	484	167	165*
			94	70	454	161
			93	51	461	166

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	94	105	66	24	30
			84	106	66	26
			94	113	95	34
			71#	76	79.5	37
Test Chemical	Tetrachloroethylene	12.5	60#	58	64	35	49*
			95#	64	149	52
			68	57	118	58
		25	91	64	127	47	50*
			70	65	99	47
			65#	46	108	56
		50	66#	37	103	52	51*
			67	51	100	50	51*
		75	8r	2	10	44	64*
			77	60	128	56
			79	45	171	72
		150	83	41	137	55	53*
			94	46	148	52
			66	16	99	50
Positive Control	3-Methylcholanthrene	2.5	70	45	519	246	241*
			66	50	504	255
			84	42	552	220

Induced S9 Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	58	71	118	68	59
			71	109	137	64
			78	96	138	59
			77	124	99	43
Test Chemical	Tetrachloroethylene	12.5	61	83	115	63	64
			65	104	141	72
			79	123	136	57
		25	72	99	131	61	64
			58	83	88	51
			62	74	152	82
		50	64	68	161	84	91*
			56	57	125	75
			61	68	210	114
		75	61	20	151	83	91*
			59	54	162	92
			71	77	212	100
		100					85
			90	37	259	96
			67	28	188	93
			64	32	125	65
		150



Positive Control	3-Methylcholanthrene	2.5	57	56	418	244	278*
			44	35	405	308
			49	41	412	281

Induced S9 Trial: 4 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	88#	103	98	37	35
			107	120	106	33
			104	82	133	42
			87	95	75	29
Test Chemical	Tetrachloroethylene	6.25	81	86	67	28	35
			80#	86	92	38
			80	99	90	38
		12.5	62#	50	68	36	35
			54	56	44	27
			80	67	103	43
		25	94#	73	112	40	40
			82	62	100	41
			62	64	71	38
		50	82	41	86	35	30
			103	77	78	25	30
		75


Positive Control	3-Methylcholanthrene	2.5	109	82	614	189	206*
			94	82	617	218
			108	70	687	211

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination