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Study Information
Study Attribute Value
CEBS Accession Number: 002-02879-0001-0000-9
Chemical Name: Tetracycline hydrochloride
CASRN: 64-75-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 931214_SCE
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Questionable 
No Activation  2  Negative 
Induced Rat Liver S9  1_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 07/03/1984    Trial Call: Questionable   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1044 443 0.424 8.86 25.7 0.000
Test Chemical Tetracycline hydrochloride 
10         
 50 1040 470 0.452 9.40 25.7 6.503 
24.8       
 50 1036 526 0.508 10.52 33.2 19.653 
49.9       
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.0015    
 50 1040 757 0.728 15.14 25.7 71.538
Mitomycin-C 
 0.01      
 5 105 281 2.676 56.20 25.7 530.687
Trend: 2.816        
Probability: 0.002        

Trial #: 2    Activation: No Activation    Date: 03/07/1985    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1034 404 0.391 8.08 25.5 0.000
Test Chemical Tetracycline hydrochloride 
5         
 50 1036 423 0.408 8.46 25.5 4.501 
10         
 50 1029 386 0.375 7.72 25.5 -3.991 
24.9       
 50 1037 400 0.386 8.00 32.2 -1.277 
40.2       
 0 0 0 0.000 0.00 32.2 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1032 565 0.547 11.30 25.5 40.123
Mitomycin-C 
 0.01      
 5 105 189 1.800 37.80 25.5 360.693
Trend: -0.533        
Probability: 0.703        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 07/03/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1041 451 0.433 9.02 25.7 0.000
Test Chemical Tetracycline hydrochloride 
302.4       
 50 1023 516 0.504 10.32 25.7 16.426 
402         
 50 1039 527 0.507 10.54 33.2 17.076 
504         
 50 1038 524 0.505 10.48 33.2 16.522 
600         
 0 0 0 0.000 0.00 33.2 N/A
Positive Control Cyclophosphamide 
 0.4       
 50 1040 632 0.608 12.64 25.7 40.268
Cyclophosphamide 
2         
 5 105 186 1.771 37.20 25.7 308.882
Trend: 2.224        
Probability: 0.013