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Study Information
Study Attribute Value
CEBS Accession Number: 002-02879-0002-0000-0
Chemical Name: Tetracycline hydrochloride
CASRN: 64-75-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 931214_CA
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Weakly Positive 
No Activation  2  Negative 
Induced Rat Liver S9  2_S9  Negative 

Trial #:1   Activation: No Activation    Date: 09/06/1984    Harvest Time: 10.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Medium 
0         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Test Chemical: Tetracycline hydrochloride 
150         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
200         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
300         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
400         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.15      
 100 11 0.110 10.0 4 0.040 4.0 7 0.070 6.0 0 0.000 0.0
Mitomycin-C 
 0.5       
 50 11 0.220 18.0 4 0.080 8.0 7 0.140 12.0 0 0.000 0.0
Trend: -1.323 0.000 -1.323  
Probability: 0.907 0.000 0.907  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 09/06/1984    Harvest Time: 20.5 hour(s)   Trial Call: Weakly Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Medium 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: Tetracycline hydrochloride 
1000         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 
1500         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0 
2000         
 200 14 0.070 7.0 0 0.000 0.0 14 0.070 7.0 0 0.000 0.0 
2500         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
6.25      
 100 15 0.150 12.0 8 0.080 8.0 7 0.070 7.0 0 0.000 0.0
Cyclophosphamide 
12.5       
 25 20 0.800 52.0 10 0.400 36.0 10 0.400 24.0 0 0.000 0.0
Trend: 2.707 -0.755 2.959  
Probability: 0.003 0.775 0.002  


Trial #:2   Activation: No Activation    Date: 08/14/1984    Harvest Time: 10.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Medium 
0         
 100 5 0.050 5.0 0 0.000 0.0 5 0.050 5.0 0 0.000 0.0
Test Chemical: Tetracycline hydrochloride 
39.9       
 100 4 0.040 4.0 1 0.010 1.0 3 0.030 3.0 0 0.000 0.0 
49.7       
 100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0 
70         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.15      
 100 8 0.080 8.0 3 0.030 3.0 5 0.050 5.0 0 0.000 0.0
Mitomycin-C 
 0.5       
 25 7 0.280 24.0 3 0.120 12.0 4 0.160 16.0 0 0.000 0.0
Trend: -1.656 0.717 -2.105  
Probability: 0.951 0.237 0.982  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 09/28/1984    Harvest Time: 21 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Medium 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: Tetracycline hydrochloride 
2260         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
2500         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
2750         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
6.25      
 100 8 0.080 7.0 5 0.050 4.0 3 0.030 3.0 0 0.000 0.0
Cyclophosphamide 
12.5       
 26 9 0.346 28.0 0 0.000 0.0 9 0.346 28.0 0 0.000 0.0
Trend: -0.270 0.000 -0.270  
Probability: 0.606 0.000 0.606