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Study Information
Study Attribute Value
CEBS Accession Number: 002-02879-0009-0000-7
Chemical Name: Tetracycline hydrochloride
CASRN: 64-75-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 615800
Study Result: Equivocal


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 92 94 104 38 40
    99 111 108 36
    107 104 109 34
    79 92 124 53
Test Chemical Tetracycline hydrochloride 
40         
 112 77 119 35 42
    102 96 127 41
    74 89 108 49
  
50         
 82 98 97 39 42
    83 66 118 47
    86 82 100 39
  
60         
 86 82 143 55 45
    69 82 82 40
    106 83 123 39
  
80         
 92 65 94 34 35
    74 69 66 30
    89 67 109 41
  
100         
 84 61 107 42 48
    75 39 121 54
    75 37 106 47
  
200         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 72 44 973 449 401*
    63 68 744 395
    61 77 661 360

Nonactivation Trial: 2 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 113 105 99 29 32
    110 102 86 26
    114 102 134 39
    98 92 96 33
Test Chemical Tetracycline hydrochloride 
50         
 82 56 81 33 31
    77 82 73 32
    103 75 90 29
  
60         
 99 84 97 33 35
    86 83 93 36
    86 80 94 36
  
80         
 123r 70 87 24 31
    88 78 83 31
    87 71 78 30
  
100         
 107 53 111 35 35
    94 67 84 30
    87 63 107 41
  
150         
 77 26 97 42 37
    80 35 93 39
    100 32 88 29
  
200         
 64 6 136 70 62*
    64 6 107 56
    78 7 139 59
Positive Control Methyl Methane Sulfonate 
5         
 71 44 582 273 280*
    67 36 526 261
    58 38 534 305

Nonactivation Trial: 3 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 53 90 53 33 29
    59 96 64 36
    71 103 41 19
    81 112 66 27
Test Chemical Tetracycline hydrochloride 
25         
 88 138 56 21 23
    116 146 74 21
    79 110 62 26
  
50         
 88 117 56 21 24
    102 107 66 21
    77 117 69 30
  
75         
 98 111 59 20 19
    82 98 58 24
    106 131 40 13
  
100         
 79 72 66 28 25
    87 70 79 30
    105 84 56 18
  
150         
 60 23 97 54 37
    72 30 61 28
    68 33 56 28
  
200         
 66 7 79 40 76*
    82 13 64 26
    18 2 88 163
  
300         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 73 79 334 152 143*
    65 68 269 139
    53 59 222 139

Non-Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 69 115 115 56 35
    90 82 79 29
    71 102 48 22
    62 101 58 31
Test Chemical Tetracycline hydrochloride 
20         
 53 91 60 38 38
    45 115 56 41
    61 82 63 35
  
30         
 62 86 97 52 48
    55 55 83 50
    68 75 83 41
  
40         
 64# 70 53 28 44
    48 41 102 70
    59 71 60 34
  
50         
   
  
60         
 65 34 94 48 66*
    42 20 84 66
    34 31 83 83
  
80         
 72*
    49 17 102 70
    44 12 98 75
  
100         
   
   
Positive Control 3-Methylcholanthrene 
3.5       
 66 51 487 246 218*
    85 80 523 206
    100 35 607 202

Non-Induced S9 Trial: 2 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 111 98 119 36 38
    93 106 118 42
    119 100 128 36
    92 96 108 39
Test Chemical Tetracycline hydrochloride 
40         
 92 48 92 33 32
    105 56 112 36
    96 65 76 26
  
50         
 89 47 127 48 44
    105# 61 135 43
    88 58 110 42
  
60         
 90 32 131 49 39
    94 41 92 33
    107 43 117 37
  
80         
 116 19 116 33 36
    100 22 83 28
    114 23 156 46
  
100         
 90 14 148 55 50
    94 15 135 48
    84 18 116 46
  
120         
 74 9 159 71 63*
    87 12 141 54
    75 11 146 65
Positive Control 3-Methylcholanthrene 
3.5       
 85 48 593 233 209*
    99 54 619 208
    95 67 529 185

Non-Induced S9 Trial: 3 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 112 112 123 37 41
    89 103 125 47
    101 86 105 35
    92 100 126 46
Test Chemical Tetracycline hydrochloride 
40         
 78 60 99 42 35
    78 62 65 28
  
60         
 78 39 83 36 32
    85 31 72 28
    64# 37 63 33
    71 23 68 32
  
80         
 76 21 57 25 43
    76 19 170 74
    86# 18 114 44
    84 21 71 28
  
100         
 85 14 137 54 49
    98 18 177 60
    87 14 110 42
    98 16 123 42
  
120         
 63 8 118 62 65*
    71 8 126 59
    78 11 184 78
    66 8 115 58
Positive Control 3-Methylcholanthrene 
3.5       
 122r 71 708 194 220*
    102 70 711 233
    96# 80 594 206

Induced S9 Trial: 1 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 94 88 67 24 32
    92 87 92 33
    104 104 103 33
    97 121 110 38
Test Chemical Tetracycline hydrochloride 
10         
 81 76 62 26 29
    74 78 63 28
    98 77 94 32
  
20         
 97 68 50 17 18
    70 75 42 20
    79 96 43 18
  
30         
 70 62 58 28 25
    102 65 77 25
    89 53 58 22
  
40         
 80 70 70 29 28
    100 81 77 26
    102 99 85 28
  
50         
 93 62 55 20 24
    78 50 66 28
    81 59 59 24
  
60         
 104 41 104 33 24
    86 56 53 21
    104 49 60 19
Positive Control 3-Methylcholanthrene 
2.5       
 58 40 410 234 224*
    83 48 609 246
    86 37 497 192

Induced S9 Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 94 100 90 32 33
    86 96 94 37
    92 91 77 28
    94 113 97 35
Test Chemical Tetracycline hydrochloride 
40         
 83 55 95 38 40
    92 66 113 41
  
50         
 110 56 70 21 24
    104 66 92 30
    108 63 67 21
  
60         
 104 51 81 26 29
    105 42 122 39
    108 58 76 23
  
80         
 100 26 98 33 38
    117 24 119 34
    90 24 126 47
  
100         
 96 16 121 42 41
    72 10 88 41
    87 15 105 40
  
120         
 77 10 147 63 49*
    103 12 102 33
    82 8 122 50
Positive Control 3-Methylcholanthrene 
2.5       
 42 15 524 419 326*
    72 25 568 265
    65 18 575 293

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination