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Study Information
Study Attribute Value
CEBS Accession Number: 002-02885-0003-0000-8
Chemical Name: Tetraethylthiuram disulfide
CASRN: 97-77-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 768765_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Weakly Positive* 
No Activation  2  Weakly Positive 
Induced Rat Liver S9  2_S9  Positive 
No Activation  3  Negative 
Induced Rat Liver S9  3_S9  Positive 

Trial #:1   Activation: No Activation    Date: 02/19/1986    Harvest Time: 15 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0
Test Chemical: Tetraethylthiuram disulfide 
 0.16      
 200 3 0.015 1.5 1 0.005 0.5 2 0.010 1.0 0 0.000 0.0 
 0.3       
 200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0 
 0.5       
 109 3 0.028 2.8 0 0.000 0.0 3 0.028 2.8 0 0.000 0.0 
1         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.0625    
 200 87 0.435 32.0 37 0.185 15.5 40 0.200 19.0 10 0.050 1.0
Mitomycin-C 
 0.125     
 200 150 0.750 49.0 90 0.450 36.0 60 0.300 22.0 0 0.000 0.0
Trend: 1.346 -0.291 1.529  
Probability: 0.089 0.614 0.063  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 05/27/1986    Harvest Time: 13 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 6 0.030 2.5 1 0.005 0.5 5 0.025 2.0 0 0.000 0.0
Test Chemical: Tetraethylthiuram disulfide 
 0.5       
 200 2 0.010 1.0 1 0.005 0.5 1 0.005 0.5 0 0.000 0.0 
1         
 200 1 0.005 0.5 1 0.005 0.5 0 0.000 0.0 0 0.000 0.0 
1.6       
 200 6 0.030 3.0 0 0.000 0.0 6 0.030 3.0 0 0.000 0.0 
3         
 200 8 0.040 4.0 1 0.005 0.5 7 0.035 3.5 0 0.000 0.0 
5         
 200 27 0.135 7.0 6 0.030 3.0 11 0.055 4.0 10 0.050 1.0 
10         
 80 19 0.238 12.5 8 0.100 6.3 11 0.138 11.3 0 0.000 0.0
Positive Control: Cyclophosphamide 
2.5       
 200 25 0.125 11.0 4 0.020 1.5 21 0.105 10.0 0 0.000 0.0
Cyclophosphamide 
7.5       
 50 17 0.340 28.0 7 0.140 14.0 10 0.200 18.0 0 0.000 0.0
Trend: 4.705 3.670 4.210  
Probability: 0.000 0.000 0.000  


Trial #:2   Activation: No Activation    Date: 02/19/1986    Harvest Time: 22 hour(s)   Trial Call: Weakly Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 1 0.005 0.5 1 0.005 0.5 0 0.000 0.0 0 0.000 0.0
Test Chemical: Tetraethylthiuram disulfide 
 0.16      
 200 5 0.025 2.5 0 0.000 0.0 5 0.025 2.5 0 0.000 0.0 
 0.3       
 73 3 0.041 2.7 2 0.027 1.4 1 0.014 1.4 0 0.000 0.0 
 0.5       
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
1         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
1.6       
 38 2 0.053 5.3 1 0.026 2.6 1 0.026 2.6 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.0625    
 200 63 0.315 23.5 29 0.145 12.5 34 0.170 14.0 0 0.000 0.0
Mitomycin-C 
 0.125     
 200 264 1.320 68.5 117 0.585 41.5 117 0.585 40.5 30 0.150 3.0
Trend: 2.076 1.697 1.333  
Probability: 0.019 0.045 0.091  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 05/27/1986    Harvest Time: 18 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 5 0.025 2.5 0 0.000 0.0 5 0.025 2.5 0 0.000 0.0
Test Chemical: Tetraethylthiuram disulfide 
1.6       
 200 2 0.010 1.0 2 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
3         
 200 7 0.035 3.0 4 0.020 2.0 3 0.015 1.0 0 0.000 0.0 
5         
 200 18 0.090 8.5 10 0.050 4.5 8 0.040 4.0 0 0.000 0.0 
10         
 135 37 0.274 20.0 16 0.119 10.4 21 0.156 11.9 0 0.000 0.0
Positive Control: Cyclophosphamide 
2.5       
 200 29 0.145 13.0 5 0.025 2.5 24 0.120 10.5 0 0.000 0.0
Cyclophosphamide 
7.5       
 50 36 0.720 36.0 13 0.260 18.0 23 0.460 28.0 0 0.000 0.0
Trend: 6.769 5.463 4.667  
Probability: 0.000 0.000 0.000  


Trial #:3   Activation: No Activation    Date: 02/19/1989    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0
Test Chemical: Tetraethylthiuram disulfide 
 0.5       
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0 
1         
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0 
1.6       
 200 6 0.030 2.5 2 0.010 1.0 4 0.020 1.5 0 0.000 0.0 
3         
 81 3 0.037 3.7 0 0.000 0.0 3 0.037 3.7 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.125     
 200 53 0.265 21.0 24 0.120 10.5 29 0.145 12.0 0 0.000 0.0
Mitomycin-C 
 0.25      
 50 21 0.420 30.0 7 0.140 8.0 14 0.280 24.0 0 0.000 0.0
Trend: 2.501 1.314 2.140  
Probability: 0.006 0.094 0.016  


Trial #:3_S9   Activation: Induced Rat Liver S9    Date: 05/27/1986    Harvest Time: 23 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0
Test Chemical: Tetraethylthiuram disulfide 
1.6       
 200 2 0.010 1.0 1 0.005 0.5 1 0.005 0.5 0 0.000 0.0 
3         
 200 10 0.050 4.5 4 0.020 2.0 6 0.030 3.0 0 0.000 0.0 
5         
 200 26 0.130 9.5 15 0.075 5.5 11 0.055 5.0 0 0.000 0.0 
10         
 118 85 0.720 33.1 25 0.212 17.8 40 0.339 19.5 20 0.169 2.0
Positive Control: Cyclophosphamide 
5         
 200 32 0.160 11.5 12 0.060 4.0 20 0.100 9.0 0 0.000 0.0
Cyclophosphamide 
7.5       
 50 16 0.320 22.0 6 0.120 6.0 10 0.200 18.0 0 0.000 0.0
Trend: 9.696 7.421 7.011  
Probability: 0.000 0.000 0.000