Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02885-0009-0000-4
Chemical Name:	Tetraethylthiuram disulfide
CASRN:	97-77-8
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	986702
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	76	100	113	50	50
			79	100	115	49
			82	102	111	45
			77	99	132	57
Test Chemical	Tetraethylthiuram disulfide	0.125	52	20	290	184	162*
			55	26	229	140	162*
		0.25	54	25	227	141	134*
			59	26	224	127	134*
		0.5	48	16	237	164	155*
			54	21	237	146	155*
		1	68	17	182	89	101*
			58	17	200	114	101*
		2	77	14	234	102	90*
			84	25	196	78	90*
		4

Positive Control	Methyl Methane Sulfonate	20	34	19	243	237	241*
			30	18	222	244	241*
	Methyl Methane Sulfonate	250	85	73	534	210	221*
			88	91	607	231	221*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	82	98	76	31	36
			85	90	106	42
			75	87	82	36
			76	126	81	35
Test Chemical	Tetraethylthiuram disulfide	0.25	53	8	359	226	205*
			62	9	343	184	205*
		0.5	69	10	352	171	188*
			54	5	328	204	188*
		1	64	16	309	160	154*
			85	12	378	148	154*
		2

Positive Control	Methyl Methane Sulfonate	20	9r	4	112	407	371*
Positive Control			10	4	115	371	371*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	93	97	197	70	45
			94	101	118	42
			95	108	155	55
			88	93	30	11
Test Chemical	Tetraethylthiuram disulfide	0.032	45#	10	679.5	501	574*
			51	11	980	647	574*
		0.063	57	7	1059	616	536*
			35	7	480	457	536*
		0.125	80	19	595	247	282*
			56	8	529	317	282*
		0.25	85	27	402	158	143*
			98	30	375	128	143*
		0.5	77	16	368	160	127*
			88	31	248	94	127*
Positive Control	Methyl Methane Sulfonate	15	40	21	253	213	214*
Positive Control			38	22	248	216	214*

Nonactivation Trial: 4 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	91	93	165	61	49
			89	109	124	46
			82	106	118	48
			88	92	113	43
Test Chemical	Tetraethylthiuram disulfide	0.016	40	10	654	543	596*
			40	9	769	649	596*
		0.032	54	13	517	320	321*
			46	12	448	322	321*
		0.063	54	27	243	149	150*
			66	27	299	151	150*
		0.125	82	55	237	96	107*
			71	51	251	118	107*
		0.25	75	64	179	80	93*
			68	48	213	105	93*
		0.5	68	57	164	81	76
			64	63	136	71	76
Positive Control	Methyl Methane Sulfonate	15	32	7	211	221	252*
Positive Control			28	15	240	282	252*

Nonactivation Trial: 5 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	114	101	256	75	61
			106	93	189	59
			103	110	181	59
			88	96	138	52
Test Chemical	Tetraethylthiuram disulfide	0.0E-4	99	101	158	53	52
			73	113	110	50	52
		0.0013	97	103	143	49	54
			83	89	144	58	54
		0.0025	88	97	135	51	56
			87	85	161	62	56
		0.005	109	83	233	72	62
			104	97	163	52	62
		0.01	95	84	158	56	59
			93	93	171	61	59
		0.02	82	42	215	87	106*
			60	32	223	124	106*
Positive Control	Methyl Methane Sulfonate	15	42	26	512	406	377*
Positive Control			54	22	558	348	377*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination