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Study Information
Study Attribute Value
CEBS Accession Number: 002-02885-0009-0000-4
Chemical Name: Tetraethylthiuram disulfide
CASRN: 97-77-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 986702
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 76 100 113 50 50
    79 100 115 49
    82 102 111 45
    77 99 132 57
Test Chemical Tetraethylthiuram disulfide 
 0.125     
 52 20 290 184 162*
    55 26 229 140
  
 0.25      
 54 25 227 141 134*
    59 26 224 127
  
 0.5       
 48 16 237 164 155*
    54 21 237 146
  
1         
 68 17 182 89 101*
    58 17 200 114
  
2         
 77 14 234 102 90*
    84 25 196 78
  
4         
   
Positive Control Methyl Methane Sulfonate 
20         
 34 19 243 237 241*
    30 18 222 244
Methyl Methane Sulfonate 
250         
 85 73 534 210 221*
    88 91 607 231

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 82 98 76 31 36
    85 90 106 42
    75 87 82 36
    76 126 81 35
Test Chemical Tetraethylthiuram disulfide 
 0.25      
 53 8 359 226 205*
    62 9 343 184
  
 0.5       
 69 10 352 171 188*
    54 5 328 204
  
1         
 64 16 309 160 154*
    85 12 378 148
  
2         
   
Positive Control Methyl Methane Sulfonate 
20         
 9r 4 112 407 371*
    10 4 115 371

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 93 97 197 70 45
    94 101 118 42
    95 108 155 55
    88 93 30 11
Test Chemical Tetraethylthiuram disulfide 
 0.032     
 45# 10 679.5 501 574*
    51 11 980 647
  
 0.063     
 57 7 1059 616 536*
    35 7 480 457
  
 0.125     
 80 19 595 247 282*
    56 8 529 317
  
 0.25      
 85 27 402 158 143*
    98 30 375 128
  
 0.5       
 77 16 368 160 127*
    88 31 248 94
Positive Control Methyl Methane Sulfonate 
15         
 40 21 253 213 214*
    38 22 248 216

Nonactivation Trial: 4 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 91 93 165 61 49
    89 109 124 46
    82 106 118 48
    88 92 113 43
Test Chemical Tetraethylthiuram disulfide 
 0.016     
 40 10 654 543 596*
    40 9 769 649
  
 0.032     
 54 13 517 320 321*
    46 12 448 322
  
 0.063     
 54 27 243 149 150*
    66 27 299 151
  
 0.125     
 82 55 237 96 107*
    71 51 251 118
  
 0.25      
 75 64 179 80 93*
    68 48 213 105
  
 0.5       
 68 57 164 81 76
    64 63 136 71
Positive Control Methyl Methane Sulfonate 
15         
 32 7 211 221 252*
    28 15 240 282

Nonactivation Trial: 5 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 114 101 256 75 61
    106 93 189 59
    103 110 181 59
    88 96 138 52
Test Chemical Tetraethylthiuram disulfide 
 0.0E-4    
 99 101 158 53 52
    73 113 110 50
  
 0.0013    
 97 103 143 49 54
    83 89 144 58
  
 0.0025    
 88 97 135 51 56
    87 85 161 62
  
 0.005     
 109 83 233 72 62
    104 97 163 52
  
 0.01      
 95 84 158 56 59
    93 93 171 61
  
 0.02      
 82 42 215 87 106*
    60 32 223 124
Positive Control Methyl Methane Sulfonate 
15         
 42 26 512 406 377*
    54 22 558 348

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination