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Study Information
Study Attribute Value
CEBS Accession Number: 002-02888-0009-0000-7
Chemical Name: Tetrakis(hydroxymethyl)phosphonium chloride
CASRN: 124-64-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 824859
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 79 81 108 46 40
    109 160 94 29
    79 105 93 39
    67 53 90 45
Test Chemical Tetrakis(hydroxymethyl)phosphonium chloride 
 0.625     
 68 107 46 23 26
    86 107 72 28
    85# 143 69 27
  
1.25      
 82 96 78 32 28
    80 77 64 27
    80 121 62 26
  
2.5       
 72 100 79 37 33
    77 77 66 29
    89 101 93 35
  
5         
 78# 81 95 41 50
    95 56 121 42
    81 65 160 66
  
7.5       
 56 19 557 334 353*
    61# 22 437 238
    46 12 673 488
  
10         
 26# 10 419 544 555*
    31 7 529 566
Positive Control Methyl Methane Sulfonate 
5         
 58 28 535 306 364*
    46 11 627 456
    57# 50 559 329

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 66 82 133 68 53
    84 104 144 57
    90 108 111 41
    85 106 115 45
Test Chemical Tetrakis(hydroxymethyl)phosphonium chloride 
3         
 72 76 123 57 46
    86 76 114 44
    83 81 89 36
  
4         
 75 78 119 53 58
    68 64 123 60
    73# 73 134 61
  
5         
 70 32 188 90 76
    86 59 198 77
    94 58 173 61
  
6         
 57 43 207 120 137*
    74 34 371 168
    57 34 209 123
  
8         
 75 39 502 223 327*
    44 11 582 441
    64 30 605 317
  
10         
 38 5 624 555 523*
    45# 12 699 524
    54# 6 794 492
Positive Control Methyl Methane Sulfonate 
5         
 38 25 655 577 554*
    55 34 701 429
    38 36 748 656

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination