Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02888-0009-0000-7
Chemical Name:	Tetrakis(hydroxymethyl)phosphonium chloride
CASRN:	124-64-1
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	824859
Study Result:	Positive

View Study Details | View Protocol | Download To Excel

Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	79	81	108	46	40
			109	160	94	29
			79	105	93	39
			67	53	90	45
Test Chemical	Tetrakis(hydroxymethyl)phosphonium chloride	0.625	68	107	46	23	26
			86	107	72	28
			85#	143	69	27
		1.25	82	96	78	32	28
			80	77	64	27
			80	121	62	26
		2.5	72	100	79	37	33
			77	77	66	29
			89	101	93	35
		5	78#	81	95	41	50
			95	56	121	42
			81	65	160	66
		7.5	56	19	557	334	353*
			61#	22	437	238
			46	12	673	488
		10	26#	10	419	544	555*
			31	7	529	566	555*
Positive Control	Methyl Methane Sulfonate	5	58	28	535	306	364*
			46	11	627	456
			57#	50	559	329

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	66	82	133	68	53
			84	104	144	57
			90	108	111	41
			85	106	115	45
Test Chemical	Tetrakis(hydroxymethyl)phosphonium chloride	3	72	76	123	57	46
			86	76	114	44
			83	81	89	36
		4	75	78	119	53	58
			68	64	123	60
			73#	73	134	61
		5	70	32	188	90	76
			86	59	198	77
			94	58	173	61
		6	57	43	207	120	137*
			74	34	371	168
			57	34	209	123
		8	75	39	502	223	327*
			44	11	582	441
			64	30	605	317
		10	38	5	624	555	523*
			45#	12	699	524
			54#	6	794	492
Positive Control	Methyl Methane Sulfonate	5	38	25	655	577	554*
			55	34	701	429
			38	36	748	656

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination