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Study Information
Study Attribute Value
CEBS Accession Number: 002-02889-0008-0000-7
Chemical Name: Tetrakis(hydroxymethyl)phosphonium sulfate
CASRN: 55566-30-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 981818
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 96 104 88 31 25
    89 90 71 27
    101 106 53 17
    132r 125 69 17
Test Chemical Tetrakis(hydroxymethyl)phosphonium sulfate 
2         
 79 91 59 25 29
    86 62 67 26
    83 55 87 35
  
3         
 104 72 94 30 27
    78 86 62 26
    88 77 64 24
  
4         
 85 44 71 28 35
    75 48 121 54
    102 49 74 24
  
5         
 89 45 112 42 40*
    104 36 128 41
    89 48 99 37
  
6         
 96 39 167 58 47*
    114 44 156 46
    109 56 121 37
  
8         
 101 28 420 139 123*
    104 31 368 118
    71 22 238 111
Positive Control Methyl Methane Sulfonate 
5         
 93 68 376 135 137*
    98 73 402 137
    106 94 439 138

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 98 135 88 30 33
    81 106 63 26
    81 72 92 38
    77 87 89 39
Test Chemical Tetrakis(hydroxymethyl)phosphonium sulfate 
3         
 66 77 69 35 33
    93 98 86 31
    88 71 87 33
  
4         
 57 61 80 47 49
    76 57 127 56
    82 40 109 44
  
6         
 85 48 168 66 60*
    94 87 153 54
    370r 342 104 9
  
8         
 70 39 270 128 173*
    99 57 386 130
    58 24 455 260
  
12         
 72 21 798 369 341*
    68 20 675 331
    66 24 634 323
  
16         
 34 4 634 625 573*
    46 12 793 575
    52 7 813 521
Positive Control Methyl Methane Sulfonate 
5         
 91 165 258 95 100*
    81 162 252 104
    86 171 259 100

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination