Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02889-0008-0000-7
Chemical Name:	Tetrakis(hydroxymethyl)phosphonium sulfate
CASRN:	55566-30-8
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	981818
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	96	104	88	31	25
			89	90	71	27
			101	106	53	17
			132r	125	69	17
Test Chemical	Tetrakis(hydroxymethyl)phosphonium sulfate	2	79	91	59	25	29
			86	62	67	26
			83	55	87	35
		3	104	72	94	30	27
			78	86	62	26
			88	77	64	24
		4	85	44	71	28	35
			75	48	121	54
			102	49	74	24
		5	89	45	112	42	40*
			104	36	128	41
			89	48	99	37
		6	96	39	167	58	47*
			114	44	156	46
			109	56	121	37
		8	101	28	420	139	123*
			104	31	368	118
			71	22	238	111
Positive Control	Methyl Methane Sulfonate	5	93	68	376	135	137*
			98	73	402	137
			106	94	439	138

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	98	135	88	30	33
			81	106	63	26
			81	72	92	38
			77	87	89	39
Test Chemical	Tetrakis(hydroxymethyl)phosphonium sulfate	3	66	77	69	35	33
			93	98	86	31
			88	71	87	33
		4	57	61	80	47	49
			76	57	127	56
			82	40	109	44
		6	85	48	168	66	60*
			94	87	153	54
			370r	342	104	9
		8	70	39	270	128	173*
			99	57	386	130
			58	24	455	260
		12	72	21	798	369	341*
			68	20	675	331
			66	24	634	323
		16	34	4	634	625	573*
			46	12	793	575
			52	7	813	521
Positive Control	Methyl Methane Sulfonate	5	91	165	258	95	100*
			81	162	252	104
			86	171	259	100

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination