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Study Information
Study Attribute Value
CEBS Accession Number: 002-02894-0002-0000-7
Chemical Name: Tetranitromethane
CASRN: 509-14-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 247313_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Weakly Positive 
Induced Rat Liver S9  2_S9  Weakly Positive* 

Trial #:1   Activation: No Activation    Date: 08/26/1986    Harvest Time: 20 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 9 0.045 4.0 4 0.020 2.0 5 0.025 2.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 200 3 0.015 1.0 1 0.005 0.5 2 0.010 0.5 0 0.000 0.0
Test Chemical: Tetranitromethane 
1.1       
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
1.5       
 200 2 0.010 1.0 2 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
3.7       
 135 2 0.015 1.5 1 0.007 0.7 1 0.007 0.7 0 0.000 0.0 
7.5       
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.05      
 200 90 0.450 19.5 31 0.155 12.0 59 0.295 14.0 0 0.000 0.0
Mitomycin-C 
 0.08      
 25 29 1.160 68.0 14 0.560 48.0 15 0.600 40.0 0 0.000 0.0
Trend: 0.652 0.486 0.285  
Probability: 0.257 0.313 0.388  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 09/23/1986    Harvest Time: 12 hour(s)   Trial Call: Weakly Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Test Chemical: Tetranitromethane 
10         
 100 4 0.040 4.0 0 0.000 0.0 4 0.040 4.0 0 0.000 0.0 
20         
 100 13 0.130 11.0 4 0.040 4.0 9 0.090 8.0 0 0.000 0.0 
29.9       
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
7.5       
 100 9 0.090 8.0 4 0.040 4.0 4 0.040 4.0 1 0.010 1.0
Cyclophosphamide 
37.5       
 25 23 0.920 44.0 6 0.240 20.0 17 0.680 40.0 0 0.000 0.0
Trend: 2.806 2.557 2.033  
Probability: 0.003 0.005 0.021  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 08/26/1986    Harvest Time: 12 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 5 0.025 2.5 2 0.010 1.0 3 0.015 1.5 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 200 3 0.015 1.5 3 0.015 1.5 0 0.000 0.0 0 0.000 0.0
Test Chemical: Tetranitromethane 
8         
 200 4 0.020 1.5 1 0.005 0.5 3 0.015 1.0 0 0.000 0.0 
19.9       
 200 2 0.010 1.0 1 0.005 0.5 1 0.005 0.5 0 0.000 0.0 
39.7       
 200 34 0.170 12.0 15 0.075 5.5 19 0.095 7.5 0 0.000 0.0 
60.2       
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
7.5       
 200 20 0.100 8.5 8 0.040 3.5 12 0.060 5.5 0 0.000 0.0
Cyclophosphamide 
37.5       
 25 25 1.000 40.0 6 0.240 16.0 19 0.760 32.0 0 0.000 0.0
Trend: 4.899 2.668 4.584  
Probability: 0.000 0.004 0.000