Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02910-0003-0000-7
Chemical Name:	Thiourea
CASRN:	62-56-6
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G70792
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: No Call
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	116	114	84	24	28
Vehicle Control			87	86	83	32	28
Test Chemical	Thiourea	786.4	127r	102	90	24
		983	110	81	123	37	32
			115	89	95	27	32
		1229	151r	116	156	34
			102	82	110	36
		1536	103	82	91	30	26
			112	89	76	23	26
		1920	107	77	126	39
			123r	77	120	33
		2400	104	67	101	32	36
			78	63	94	40	36
		3000	122r	57	125	34
			102	57	129	42
Positive Control	Ethyl Methane Sulfonate	500	1r	0	887	39422	584*
Positive Control			51	31	888	584	584*

Nonactivation Trial: 2 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	99	112	98	33	46
Vehicle Control			78	88	139	60	46
Test Chemical	Thiourea	983	78	81	93	40	39
			88	87	100	38	39
		1229	80	84	51	21	29
			94	87	101	36	29
		1536	74	71	70	32	32
			72	72	69	32	32
		1920	90	92	119	44	42
			97	74	117	40	42
		2400	75	57	78	35	36
			87	71	98	37	36
		3000	85	41	160	63
Positive Control	Ethyl Methane Sulfonate	500	42	31	767	616	616*

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	94	99	84	30	32
			92	104	66	24
			81	97	102	42
Test Chemical	Thiourea	1638	105	96	142	45	41
			98	107	110	37	41
		2048	97	76	96	33	42
			89	69	95	36
			93	25	159	57
		3200	103	84	139	45	45
			81	65	119	49
			92	77	113	41
		4000	75	58	78	35	44
			87	61	120	46
			94	55	140	50
		5000	92	27	108	39	48
			89	35	132	50
			87	49	146	56
Positive Control	Ethyl Methane Sulfonate	500	28	9	1009	1194	1312*
			22	8	950	1450
			28	11	1071	1290

Nonactivation Trial: 4 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	57	113	82	48	50
			50r	77	79	53
			58	92	87	50
			53	94	80	51
Test Chemical	Thiourea	1629	70	89	78	37	54
			79	88	154	65
			72	72	128	59
		1913	71	302	82	39	55
			60	61	115	64
			68	87	127	62
		2245	61	67	121	67	66
			64	72	129	67
			56	53	107	63
		2635	77	77	101	44	50
			60	69	81	45
			60	58	110	61
		3092	66	66	98	50	62
			64	65	123	65
			51	61	108	71
		3630	71	78	105	49	53
			79	97	137	58	53
		4260	66	68	121	61	71
			58	54	147	85
			57	72	115	68
		5000	65	59	157	80	101*
			47	43	185	132
			49	47	135	92
Positive Control	Ethyl Methane Sulfonate	500	31	34	736	796	810*
			37	47	849	772
			38	49	969	861

Non-Induced S9 Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	71	86	111	52	56
			84	97	150	60
			83	109	141	57
			84	108	138	55
Test Chemical	Thiourea	503	107	96	134	42	45
			102	96	149	49	45
		629	79	85	120	51	52
			92	117	144	52	52
		786	84	85	135	54	51
			113	136	164	49	51
		983	88	92	158	60	53
			93	109	127	45	53
		1229	106	122	145	45	43
			82	87	101	41	43
		1536	89	114	151	57	55
			90	94	141	53	55
		1920	76	81	161	71	67
			94	82	176	63	67
		2400	91	98	156	57	50
			86	90	114	44	50
		3000	76	77	142	63	57
			111	110	170	51	57
Positive Control	3-Methylcholanthrene	7	85	76	495	193	235*
Positive Control			74	71	614	277	235*

Non-Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	88	109	80	30	35
			80	88	85	36
			91	104	96	35
			80	99	90	38
Test Chemical	Thiourea	839	93	111	96	34	39
			92	93	118	43	39
		1048	97	110	114	39	40
			82	97	105	43
			95	108	104	36
		1638	79	86	118	50	47
			89	101	118	44	47
		2048	93	98	61	22	26
			94	98	87	31
			77	81	60	26
		2560	93	98	88	32	30
			93	101	69	25
			89	90	87	33
		3200	84	76	100	40	39
			80	74	94	39	39
		5000	75	36	117	52	47
			88	58	122	46
			84	57	105	42
Positive Control	3-Methylcholanthrene	7	67	60	404	201	212*
			61	51	434	238
			69	59	409	197

Induced S9 Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	83	98	136	55	56
Vehicle Control			87	102	149	57	56
Test Chemical	Thiourea	629.1	85	89	213	83	85
			77	96	200	86	85
		786.4	92	98	257	93	91*
			78	88	208	89	91*
		983	77	88	132	57	72
			79	84	202	86	72
		1229	84	87	209	83	82
			75	79	184	81	82
		1536	83	88	172	69
		1920	73	80	210	97	95*
			75	82	211	94	95*
		2400	80	76	257	107	99*
			86	84	233	91	99*
		3000	89	92	190	71	73
			86	89	193	75	73
Positive Control	3-Methylcholanthrene	5	44	27	458	344	349*
Positive Control			42	24	441	353	349*

Induced S9 Trial: 3 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	88	112	157	59	71
			86	97	200	77
			81	96	199	82
			82	95	157	64
Test Chemical	Thiourea	1638	63	64	98	52	67
			76	82	261	115
			78	84	79	34
		2048	79#	78	121.5	51	80
			67	70	281	139
			79#	98	117	49
		2560	62	51	220	118	90
			84#	87	271.5	108
			76	68	99	44
		3200	73	75	98	45	50
			87	99	145	56	50
		4000	95	98	248	87	99
			90	90	265	98
			80	72	270	113
		5000	89	89	219	82	83
			88	96	201	76
			90	73	244	90
Positive Control	3-Methylcholanthrene	5	63	52	566	298	339*
			62#	54	724.5	393
			61	43	598	328

Induced S9 Trial: 4 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	74	106	191	87	73
			64	92	115	60
			72	94	166	77
			77	108	155	68
Test Chemical	Thiourea	1310	54	71	233	143	126*
			62	84	238	128
			68	93	217	106
		1640	73#	98	114	52	81
			66	86	218	110	81
		2050	77	105	92	40	39
			69#	96	96	47
			67	85	60	30
		2560	60	83	130	72	63
			61	77	110	60
			61	77	104	57
		3200	67	83	106	53	63
			66	83	131	66
			69	90	147	71
		4000	63	75	152	80	95
			60	72	169	94
			69#	77	229.5	111
		5000	67	80	151	75	69
			78#	96	133.5	57
			69	82	153	74
Positive Control	3-Methylcholanthrene	5	44	39	497	379	375*
Positive Control			44	41	489	370	375*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination