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Study Information
Study Attribute Value
CEBS Accession Number: 002-02910-0003-0000-7
Chemical Name: Thiourea
CASRN: 62-56-6
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G70792
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: No Call
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 116 114 84 24 28
    87 86 83 32
Test Chemical Thiourea 
786.4       
 127r 102 90 24
  
983         
 110 81 123 37 32
    115 89 95 27
  
1229         
 151r 116 156 34
    102 82 110 36
  
1536         
 103 82 91 30 26
    112 89 76 23
  
1920         
 107 77 126 39
    123r 77 120 33
  
2400         
 104 67 101 32 36
    78 63 94 40
  
3000         
 122r 57 125 34
    102 57 129 42
Positive Control Ethyl Methane Sulfonate 
500         
 1r 0 887 39422 584*
    51 31 888 584

Nonactivation Trial: 2 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 99 112 98 33 46
    78 88 139 60
Test Chemical Thiourea 
983         
 78 81 93 40 39
    88 87 100 38
  
1229         
 80 84 51 21 29
    94 87 101 36
  
1536         
 74 71 70 32 32
    72 72 69 32
  
1920         
 90 92 119 44 42
    97 74 117 40
  
2400         
 75 57 78 35 36
    87 71 98 37
  
3000         
 85 41 160 63
Positive Control Ethyl Methane Sulfonate 
500         
 42 31 767 616 616*

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 94 99 84 30 32
    92 104 66 24
    81 97 102 42
Test Chemical Thiourea 
1638         
 105 96 142 45 41
    98 107 110 37
  
2048         
 97 76 96 33 42
    89 69 95 36
    93 25 159 57
  
3200         
 103 84 139 45 45
    81 65 119 49
    92 77 113 41
  
4000         
 75 58 78 35 44
    87 61 120 46
    94 55 140 50
  
5000         
 92 27 108 39 48
    89 35 132 50
    87 49 146 56
Positive Control Ethyl Methane Sulfonate 
500         
 28 9 1009 1194 1312*
    22 8 950 1450
    28 11 1071 1290

Nonactivation Trial: 4 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 57 113 82 48 50
    50r 77 79 53
    58 92 87 50
    53 94 80 51
Test Chemical Thiourea 
1629         
 70 89 78 37 54
    79 88 154 65
    72 72 128 59
  
1913         
 71 302 82 39 55
    60 61 115 64
    68 87 127 62
  
2245         
 61 67 121 67 66
    64 72 129 67
    56 53 107 63
  
2635         
 77 77 101 44 50
    60 69 81 45
    60 58 110 61
  
3092         
 66 66 98 50 62
    64 65 123 65
    51 61 108 71
  
3630         
 71 78 105 49 53
    79 97 137 58
  
4260         
 66 68 121 61 71
    58 54 147 85
    57 72 115 68
  
5000         
 65 59 157 80 101*
    47 43 185 132
    49 47 135 92
Positive Control Ethyl Methane Sulfonate 
500         
 31 34 736 796 810*
    37 47 849 772
    38 49 969 861

Non-Induced S9 Trial: 1 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 71 86 111 52 56
    84 97 150 60
    83 109 141 57
    84 108 138 55
Test Chemical Thiourea 
503         
 107 96 134 42 45
    102 96 149 49
  
629         
 79 85 120 51 52
    92 117 144 52
  
786         
 84 85 135 54 51
    113 136 164 49
  
983         
 88 92 158 60 53
    93 109 127 45
  
1229         
 106 122 145 45 43
    82 87 101 41
  
1536         
 89 114 151 57 55
    90 94 141 53
  
1920         
 76 81 161 71 67
    94 82 176 63
  
2400         
 91 98 156 57 50
    86 90 114 44
  
3000         
 76 77 142 63 57
    111 110 170 51
Positive Control 3-Methylcholanthrene 
7         
 85 76 495 193 235*
    74 71 614 277

Non-Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 88 109 80 30 35
    80 88 85 36
    91 104 96 35
    80 99 90 38
Test Chemical Thiourea 
839         
 93 111 96 34 39
    92 93 118 43
  
1048         
 97 110 114 39 40
    82 97 105 43
    95 108 104 36
  
1638         
 79 86 118 50 47
    89 101 118 44
  
2048         
 93 98 61 22 26
    94 98 87 31
    77 81 60 26
  
2560         
 93 98 88 32 30
    93 101 69 25
    89 90 87 33
  
3200         
 84 76 100 40 39
    80 74 94 39
  
5000         
 75 36 117 52 47
    88 58 122 46
    84 57 105 42
Positive Control 3-Methylcholanthrene 
7         
 67 60 404 201 212*
    61 51 434 238
    69 59 409 197

Induced S9 Trial: 1 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 83 98 136 55 56
    87 102 149 57
Test Chemical Thiourea 
629.1       
 85 89 213 83 85
    77 96 200 86
  
786.4       
 92 98 257 93 91*
    78 88 208 89
  
983         
 77 88 132 57 72
    79 84 202 86
  
1229         
 84 87 209 83 82
    75 79 184 81
  
1536         
 83 88 172 69
  
1920         
 73 80 210 97 95*
    75 82 211 94
  
2400         
 80 76 257 107 99*
    86 84 233 91
  
3000         
 89 92 190 71 73
    86 89 193 75
Positive Control 3-Methylcholanthrene 
5         
 44 27 458 344 349*
    42 24 441 353

Induced S9 Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 88 112 157 59 71
    86 97 200 77
    81 96 199 82
    82 95 157 64
Test Chemical Thiourea 
1638         
 63 64 98 52 67
    76 82 261 115
    78 84 79 34
  
2048         
 79# 78 121.5 51 80
    67 70 281 139
    79# 98 117 49
  
2560         
 62 51 220 118 90
    84# 87 271.5 108
    76 68 99 44
  
3200         
 73 75 98 45 50
    87 99 145 56
  
4000         
 95 98 248 87 99
    90 90 265 98
    80 72 270 113
  
5000         
 89 89 219 82 83
    88 96 201 76
    90 73 244 90
Positive Control 3-Methylcholanthrene 
5         
 63 52 566 298 339*
    62# 54 724.5 393
    61 43 598 328

Induced S9 Trial: 4 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 74 106 191 87 73
    64 92 115 60
    72 94 166 77
    77 108 155 68
Test Chemical Thiourea 
1310         
 54 71 233 143 126*
    62 84 238 128
    68 93 217 106
  
1640         
 73# 98 114 52 81
    66 86 218 110
  
2050         
 77 105 92 40 39
    69# 96 96 47
    67 85 60 30
  
2560         
 60 83 130 72 63
    61 77 110 60
    61 77 104 57
  
3200         
 67 83 106 53 63
    66 83 131 66
    69 90 147 71
  
4000         
 63 75 152 80 95
    60 72 169 94
    69# 77 229.5 111
  
5000         
 67 80 151 75 69
    78# 96 133.5 57
    69 82 153 74
Positive Control 3-Methylcholanthrene 
5         
 44 39 497 379 375*
    44 41 489 370

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination