Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02912-0010-0000-7
Chemical Name:	Titanium dioxide
CASRN:	13463-67-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	006080
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	105	88	102	32	35
			109	91	104	32
			101	120	125	41
			156r	139	91	19
Test Chemical	Titanium dioxide	3.13	116	119	127	37	32
			109	123	87	27
			125r	154	96	26
		6.25	108	128	99	31	26
			113	131	81	24
			113	102	77	23
		12.5	117	111	88	25	25
			124r	67	77	21
			118	46	89	25
		25	100	93	79	26	30
			115	108	100	29
			99	69	102	34
		50	105	90	118	38	30
			118	71	100	28
			117	123	88	25
		100	105	85	109	35	37
			110	44	126	38
			186r	150	82	15
Positive Control	Methyl Methane Sulfonate	5	52	8	418	265	254*
			64	15	535	277
			97	68	634	219

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	107	116	108	34	30
			116	57	87	25
			106	103	101	32
			106	138	89	28
Test Chemical	Titanium dioxide	1.56	109	97	98	30	27
			99	59	77	26
			106	87	83	26
		3.13	77	96	54	23	25
			111	98	82	25
			96	92	75	26
		6.25	109	89	65	20	27
			93	78	93	33
			104	108	88	28
		12.5	118	92	94	27	24
			102	119	70	23
			110	112	72	22
		25	95	71	86	30	30
			79	83	65	27
			113	92	107	31
		50	112	97	109	32	32
			135r	124	87	22
			110	78	104	32
Positive Control	Methyl Methane Sulfonate	5	81	46	620	255	286*
			89	53	702	263
			65	55	664	341

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	99	99	80	27	27
			118	129	58	16
			97	108	72	25
			66	63	76	38
Test Chemical	Titanium dioxide	1.56	91	106	67	24	28
			116	122	87	25
			103	109	110	36
		3.13	121r	92	85	23	32
			85	54	75	29
			94	86	96	34
		6.25	129r	98	104	27
			115r	99	69	20
			139r	107	110	26
		12.5	138r	143	80	19
			112r	76	95	28
			132r	83	101	26
		25	117	106	75	21	21
			97	116	55	19
			104	110	67	22
		50	110	112	77	23	23
			103	97	68	22
			128r	113	59	15
Positive Control	3-Methylcholanthrene	2.5	83	53	507	204	180*
Positive Control			96	81	451	157	180*

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	107	112	72	22	30
			105	89	120	38
			87#	81	80	31
			106	118	86	27
Test Chemical	Titanium dioxide	1.56	97#	89	91	31	33
			109	91	96	29
			113	70	131	39
		3.13	117	90	131	37	49*
			109	95	167	51
			98	84	176	60
		6.25	107	89	101	31	38
			118	86	150	42
			103	85	125	41
		12.5	110	103	128	39	39
			115#	101	137	40
			117	92	130	37
		25	118	91	132	37	39
			121r	71	178	49
			117	93	145	41
		50	117r	87	116	33
			121r	71	147	40
			123r	96	102	28
Positive Control	3-Methylcholanthrene	2.5	59	30	915	514	438*
			62	50	880	473
			101#	42	986	326

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination