Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02914-0008-0000-6
Chemical Name:	Tolazamide
CASRN:	1156-19-0
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	255587
Study Result:	Negative

View Study Details | View Protocol | Download To Excel

Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	86	106	73	28	30
			79	94	79	33
			81	102	75	31
			79	97	63	27
Test Chemical	Tolazamide	162.5	90	108	75	28	27
			83	88	65	26	27
		325	77	74	80	35	34
			88	98	86	33	34
		650	90	88	91	34	34
			89	82	89	33	34
		1300	91	77	97	36	35
			97	82	101	35	35
		2600	75	23	102	45	42
			77	39	91	40	42
Positive Control	Methyl Methane Sulfonate	20	18	9	83	158	183*
Positive Control			16	9	97	209	183*

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	75	93	83	37	28
			72	89	30	14
			94	102	69	25
			91	116	102	37
Test Chemical	Tolazamide	125	81	109	66	27	32
			96	119	105	36	32
		250	89	89	93	35	39
			90	101	119	44	39
		500	88	108	112	43	42
			102	90	128	42	42
		1000	107	61	126	39	43
			84	68	117	47	43
		2000	85	64	101	40	36
			96	54	93	32	36
Positive Control	Methyl Methane Sulfonate	15	28	23	93	110	99*
Positive Control			41	26	109	89	99*

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	129r	95	215	55	56
			82	100	163	67
			98	101	164	56
			113	100	159	47
Test Chemical	Tolazamide	125	74	92	156	70	61
			95	99	146	51	61
		250	93	108	164	59	59
			89	119	157	59	59
		500	86	88	133	52	49
			91	86	129	47	49
		1000	88	83	173	66	58
			98	97	151	51	58
		2000	83	72	147	59	69
			79	63	188	80	69
Positive Control	3-Methylcholanthrene	2.5	71	28	702	331	373*
Positive Control			57	35	712	414	373*

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	67	98	194	97	90
			58	104	177	103
			68	95	176	86
			83	102	185	74
Test Chemical	Tolazamide	125	55	70	222	134	138
			50#	65	211	142	138
		250	51#	48	211	137	123
			60#	65	195	109	123
		500	65#	107	192	98	92
			62#	105	160	86	92
		1000	71#	77	228	107	119
			57#	70	225	131	119
		2000	87#	37	246	95	116
			57#	15	234	138	116
Positive Control	3-Methylcholanthrene	2.5	27	10	553	687	680*
Positive Control			23	16	468	673	680*

Induced S9 Trial: 3 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	86	112	105	41	35
			76	80	78	34
			82	119	72	29
			79	89	82	35
Test Chemical	Tolazamide	187.5	59	98	75	43	44
			72	111	96	45	44
		375	87	104	99	38	41
			74	93	98	44	41
		750	62	84	96	51	49
			72	104	100	46	49
		1500	71	71	122	57	51
			69	82	91	44	51
		3000	77	29	126	55	61*
			56	21	111	66	61*
Positive Control	3-Methylcholanthrene	2.5	41	39	414	338	326*
Positive Control			57	46	537	313	326*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination