COVID-19 is an emerging, rapidly evolving situation.

Get the latest public health information from CDC and research information from NIH.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Share This:
Study Information
Study Attribute Value
CEBS Accession Number: 002-02916-0001-0000-1
Chemical Name: Toluene
CASRN: 108-88-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 638654_SCE
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
No Activation  2  Negative 
Induced Rat Liver S9  1_S9  Negative 
Induced Rat Liver S9  2_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 07/10/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1051 490 0.466 9.80 27.0 0.000
Test Chemical Toluene 
50         
 50 1050 423 0.403 8.46 27.0 -13.591 
160         
 50 1045 492 0.471 9.84 27.0 0.985 
500         
 50 1034 496 0.480 9.92 27.0 2.889 
1600         
 0 0 0 0.000 0.00 0.0 N/A 
5000         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.0E-4    
 50 1050 753 0.717 15.06 27.0 53.820
Mitomycin-C 
 0.01      
 10 210 572 2.724 57.20 27.0 484.229
Trend: 1.157        
Probability: 0.124        

Trial #: 2    Activation: No Activation    Date: 08/21/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1052 436 0.414 8.72 26.5 0.000
Test Chemical Toluene 
100         
 50 1047 460 0.439 9.20 26.5 6.008 
200         
 50 1044 426 0.408 8.52 26.5 -1.545 
300         
 50 1038 474 0.457 9.48 26.5 10.182 
400         
 0 0 0 0.000 0.00 0.0 N/A 
4000         
 0 0 0 0.000 0.00 0.0 N/A 
5000         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.0E-4    
 50 1047 632 0.604 12.64 26.0 45.646
Mitomycin-C 
 0.01      
 10 210 446 2.124 44.60 26.5 412.442
Trend: 0.916        
Probability: 0.180        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 07/10/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1051 436 0.415 8.72 26.5 0.000
Test Chemical Toluene 
16         
 50 1046 433 0.414 8.66 26.5 -0.213 
50         
 50 1045 416 0.398 8.32 26.5 -4.039 
160         
 50 1045 457 0.437 9.14 26.5 5.418 
500         
 22 459 205 0.447 9.32 26.5 7.661 
1600         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.3       
 50 1042 759 0.728 15.18 26.5 75.586
Cyclophosphamide 
2         
 10 211 480 2.275 48.00 26.5 448.372
Trend: 1.026        
Probability: 0.152        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 08/07/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1044 413 0.396 8.26 26.0 0.000
Test Chemical Toluene 
50         
 50 1044 396 0.379 7.92 26.0 -4.116 
100         
 50 1046 413 0.395 8.26 26.0 -0.191 
250         
 50 1049 413 0.394 8.26 26.0 -0.477 
500         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.3       
 50 1050 605 0.576 12.10 26.0 45.652
Cyclophosphamide 
2         
 10 209 414 1.981 41.40 26.0 400.731
Trend: 0.110        
Probability: 0.456