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Study Information
Study Attribute Value
CEBS Accession Number: 002-02916-0001-0000-1
Chemical Name: Toluene
CASRN: 108-88-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 638654_SCE
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  Negative 
No Activation  Negative 
Induced Rat Liver S9  1_S9  Negative 
Induced Rat Liver S9  2_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 07/10/1984    Trial Call: Negative   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1051 490 0.466 9.80 27.0 0.000
Test Chemical Toluene
50         
50 1050 423 0.403 8.46 27.0 -13.591
160         
50 1045 492 0.471 9.84 27.0 0.985
500         
50 1034 496 0.480 9.92 27.0 2.889
1600         
0 0 0 0.000 0.00 0.0 N/A
5000         
0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C
 0.0E-4    
50 1050 753 0.717 15.06 27.0 53.820
Mitomycin-C
 0.01      
10 210 572 2.724 57.20 27.0 484.229
Trend: 1.157        
Probability: 0.124        

Trial #: 2    Activation: No Activation    Date: 08/21/1984    Trial Call: Negative   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1052 436 0.414 8.72 26.5 0.000
Test Chemical Toluene
100         
50 1047 460 0.439 9.20 26.5 6.008
200         
50 1044 426 0.408 8.52 26.5 -1.545
300         
50 1038 474 0.457 9.48 26.5 10.182
400         
0 0 0 0.000 0.00 0.0 N/A
4000         
0 0 0 0.000 0.00 0.0 N/A
5000         
0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C
 0.0E-4    
50 1047 632 0.604 12.64 26.0 45.646
Mitomycin-C
 0.01      
10 210 446 2.124 44.60 26.5 412.442
Trend: 0.916        
Probability: 0.180        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 07/10/1984    Trial Call: Negative   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1051 436 0.415 8.72 26.5 0.000
Test Chemical Toluene
16         
50 1046 433 0.414 8.66 26.5 -0.213
50         
50 1045 416 0.398 8.32 26.5 -4.039
160         
50 1045 457 0.437 9.14 26.5 5.418
500         
22 459 205 0.447 9.32 26.5 7.661
1600         
0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide
 0.3       
50 1042 759 0.728 15.18 26.5 75.586
Cyclophosphamide
2         
10 211 480 2.275 48.00 26.5 448.372
Trend: 1.026        
Probability: 0.152        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 08/07/1984    Trial Call: Negative   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1044 413 0.396 8.26 26.0 0.000
Test Chemical Toluene
50         
50 1044 396 0.379 7.92 26.0 -4.116
100         
50 1046 413 0.395 8.26 26.0 -0.191
250         
50 1049 413 0.394 8.26 26.0 -0.477
500         
0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide
 0.3       
50 1050 605 0.576 12.10 26.0 45.652
Cyclophosphamide
2         
10 209 414 1.981 41.40 26.0 400.731
Trend: 0.110        
Probability: 0.456