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Study Information
Study Attribute Value
CEBS Accession Number: 002-02916-0009-0000-9
Chemical Name: Toluene
CASRN: 108-88-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 434629
Study Result: Equivocal


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 67 105 98 49 62
    51 66 106 70
    65 92 123 63
    77 137 156 68
Test Chemical Toluene 
31.25      
 66 74 111 56 62
    72 75 147 68
  
62.5       
 62 67 150 81 68
    57 66 94 55
  
125         
 69 111 132 64 63
    63 71 117 62
  
250         
 73 28 258 118 114*
    60 28 198 111
  
500         
   
Positive Control Methyl Methane Sulfonate 
15         
 41 48 476 387 401*
    34 29 417 415

Nonactivation Trial: 2 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 77 97 162 70 56
    76 82 89 39
    71 102 131 62
    75 120 122 54
Test Chemical Toluene 
50         
 93 117 160 57 62
    59 91 126 71
    75 105 129 57
  
100         
 71 114 132 62 57
    82 81 162 66
    74 92 98 44
  
200         
 99 65 217 73 87*
    60 53 166 93
    87 63 244 94
  
300         
   
   
Positive Control Methyl Methane Sulfonate 
15         
 20 15 287 482 463*
    24 12 321 443

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 64 92 129 67 59
    59 98 109 62
    83 114 126 51
    65 97 113 58
Test Chemical Toluene 
150         
 80 77 177 74 77
    57 56 137 80
  
175         
 72 75 130 60 81
    68 53 206 102
  
200         
 84 50 210 84 103*
    76 32 276 122
  
225         
 68 16 428 211 184*
    57 26 267 157
  
250         
 53 8 548 342 275*
    64 19 395 207
  
275         
   
Positive Control Methyl Methane Sulfonate 
15         
 20 14 255 421 428*
    27 19 352 435

Induced S9 Trial: 1 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 116 102 122 35 42
    99# 95 125 42
    90 102 118 44
    77 101 107 46
Test Chemical Toluene 
6.25      
 97 108 115 39 48
    85 92 145 57
  
12.5       
 89# 102 127 48 50
    96 112 154 53
  
25         
 78 78 122 52 51
    90# 87 132 49
  
50         
 111 93 142 43 45
    77 104 111 48
  
100         
 91 101 106 39 47
    85 83 140 55
  
200         
 71 39 174 81 73*
    78 42 150 64
Positive Control 3-Methylcholanthrene 
2.5       
 59 77 332 187 169*
    79 90 362 152

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 45r 66 66 49 68
    65 104 138 70
    61 96 119 65
    42r 72 96 76
Test Chemical Toluene 
125         
 65 76 118 61 68
    66 77 151 76
  
150         
 60 71 122 68 68
    64 76 132 68
  
175         
 56 58 131 79 75
    50 59 106 71
  
200         
 52 37 139 90 98
    43 34 136 107
  
225         
 50 18 217 144 138*
    41 18 162 132
  
250         
   
Positive Control 3-Methylcholanthrene 
2.5       
 33 26 593 593 635*
    27 28 548 677

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination