Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02916-0009-0000-9
Chemical Name:	Toluene
CASRN:	108-88-3
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	434629
Study Result:	Equivocal

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	67	105	98	49	62
			51	66	106	70
			65	92	123	63
			77	137	156	68
Test Chemical	Toluene	31.25	66	74	111	56	62
			72	75	147	68	62
		62.5	62	67	150	81	68
			57	66	94	55	68
		125	69	111	132	64	63
			63	71	117	62	63
		250	73	28	258	118	114*
			60	28	198	111	114*
		500

Positive Control	Methyl Methane Sulfonate	15	41	48	476	387	401*
Positive Control			34	29	417	415	401*

Nonactivation Trial: 2 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	77	97	162	70	56
			76	82	89	39
			71	102	131	62
			75	120	122	54
Test Chemical	Toluene	50	93	117	160	57	62
			59	91	126	71
			75	105	129	57
		100	71	114	132	62	57
			82	81	162	66
			74	92	98	44
		200	99	65	217	73	87*
			60	53	166	93
			87	63	244	94
		300


Positive Control	Methyl Methane Sulfonate	15	20	15	287	482	463*
Positive Control			24	12	321	443	463*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	64	92	129	67	59
			59	98	109	62
			83	114	126	51
			65	97	113	58
Test Chemical	Toluene	150	80	77	177	74	77
			57	56	137	80	77
		175	72	75	130	60	81
			68	53	206	102	81
		200	84	50	210	84	103*
			76	32	276	122	103*
		225	68	16	428	211	184*
			57	26	267	157	184*
		250	53	8	548	342	275*
			64	19	395	207	275*
		275

Positive Control	Methyl Methane Sulfonate	15	20	14	255	421	428*
Positive Control			27	19	352	435	428*

Induced S9 Trial: 1 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	116	102	122	35	42
			99#	95	125	42
			90	102	118	44
			77	101	107	46
Test Chemical	Toluene	6.25	97	108	115	39	48
			85	92	145	57	48
		12.5	89#	102	127	48	50
			96	112	154	53	50
		25	78	78	122	52	51
			90#	87	132	49	51
		50	111	93	142	43	45
			77	104	111	48	45
		100	91	101	106	39	47
			85	83	140	55	47
		200	71	39	174	81	73*
			78	42	150	64	73*
Positive Control	3-Methylcholanthrene	2.5	59	77	332	187	169*
Positive Control			79	90	362	152	169*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	45r	66	66	49	68
			65	104	138	70
			61	96	119	65
			42r	72	96	76
Test Chemical	Toluene	125	65	76	118	61	68
			66	77	151	76	68
		150	60	71	122	68	68
			64	76	132	68	68
		175	56	58	131	79	75
			50	59	106	71	75
		200	52	37	139	90	98
			43	34	136	107	98
		225	50	18	217	144	138*
			41	18	162	132	138*
		250

Positive Control	3-Methylcholanthrene	2.5	33	26	593	593	635*
Positive Control			27	28	548	677	635*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination