Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02945-0023-0000-7
Chemical Name:	Trichloroethylene
CASRN:	79-01-6
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	114328
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call:
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	76	121	108	47	53
Vehicle Control			76	79	135	59	53
Test Chemical	Trichloroethylene	0.01	100	92	169	57	46
			73	51	78	35	46
		0.1	113	123	126	37	89
			62	59	262	142	89
		0.3	80	39	177	74	77
			56	31	135	80	77
		0.5

Positive Control	Hycanthone	10	55	34	614	370	392*
			41	21	509	414	392*
	Hycanthone	500	43	36	1100	859	1027*
			21	19	758	1194	1027*

Induced S9 Trial: 1 Experiment Call:
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	70	98	211	100	93
Vehicle Control			78	102	203	87	93
Test Chemical	Trichloroethylene	0.01	52	65	183	117	106
			58	74	164	94	106
		0.04	43	55	289	224	185*
			55	58	238	145	185*
		0.07	46	44	280	203	190*
			58	54	308	178	190*
		0.1	52	45	418	270	284*
			45	34	403	297	284*
		0.14	49	24	306	207	243*
			48	18	402	279	243*
		0.17	50	18	460	309	275*
			57	20	413	242	275*
		0.2	45	11	331	247	288*
			50	13	493	330	288*
		0.5

Positive Control	3-Methylcholanthrene	6	66	87	562	284	291*
Positive Control			69	92	618	298	291*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination