Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02945-0024-0000-8
Chemical Name:	Trichloroethylene
CASRN:	79-01-6
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	623351
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	112	117	60	18	19
			105	76	73	23
			100#	107	45	15
Test Chemical	Trichloroethylene	0.0078	80#	87	61.5	26	24
			91	72	71	26
			86	75	54	21
		0.0156	112#	109	55.5	17	17
			89	100	36	13
			84#	70	55.5	22
		0.0313	78	108	35	15	19
			64#	60	43	22	19
		0.0625	102	96	49	16	16
			85	105	38	15
			83#	69	43.5	18
		0.125	82	97	50	20	13
			98	87	26	9
			92	92	28	10
		0.25	97	66	45	15	15
			104	72	39	12
			102	47	51	17
		0.5


Positive Control	Ethyl Methane Sulfonate	250	65#	64	796.5	411	388*
Positive Control			93	64	1018	366	388*

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	105	100	94	30	24
			94	98	74	26
			115	110	68	20
			112	92	68	20
Test Chemical	Trichloroethylene	0.025	103	95	69	22	23
			99	86	84	28
			95	89	56	20
		0.05	111	73	83	25	23
			92	66	74	27
			95	93	49	17
		0.1	100	62	46	15	17
			97	81	53	18
			124r	66	107	29
		0.2	80	17	58	24	23
			105	45	72	23
			90	56	63	23
		0.3	105	23	53	17	22

			94	20	78	28
		0.4


Positive Control	Ethyl Methane Sulfonate	250	61#	40	957	524	549*
			68	34	1188	584
			69#	46	1117.5	540

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	111	113	74	22	23
			135r	67	59	15
			120r	103	49	14
			90#	87	63	23
Test Chemical	Trichloroethylene	0.0156	98	68	69	24	27
			93	91	70	25
			69#	64	66	32
		0.0313	105#	83	82	26	24
			113#	101	81	24
			99	60	62	21
		0.0625	97	75	78	27	23
			91	66	64	23
			111#	84	59	18
		0.125	86#	43	99	38	30
			112	43	115	34
			107	68	58	18
		0.25	103	38	113	37	39*
			88#	15	117	44
			103	23	113	37
Positive Control	3-Methylcholanthrene	2.5	101	33	444	147	179*
			90	40	474	176
			76	39	485	214

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	106#	81	261	82	71
			113	102	233	69
			93	114	178	64
			107	102	227	71
Test Chemical	Trichloroethylene	0.025	99	66	331	112	101
			105	74	308	98
			96	74	265	92
		0.05	106	56	342	108	97
			108	61	311	96
			108	60	284	88
		0.1	90	48	403	150	128*
			82	39	257	105
			100	39	382	128
		0.2	94	20	448	158	160*
			100	16	441	147
			95	17	498	175
		0.3					169*
			88	10	382	145
			74	8	427	192
		0.4


Positive Control	3-Methylcholanthrene	2.5	80	22	775	325	315*
			91	45	813	299
			83#	35	802.5	322

Induced S9 Trial: 3 Experiment Call:
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	104#	82	175.5	56	49
			112	103	157	47
			92	114	131	48
			105	102	150	48
Test Chemical	Trichloroethylene	0.025	98	66	206	70	65
			94	73	168	59
			104	74	199	64
		0.05	107	60	202	63	65
			107	61	201	63
			104	56	219	70
		0.1	80	38	169	71	87*
			99	39	255	86
			88	48	271	103
		0.2	99	16	282	95	110*
			93	20	295	106
			85	16	328	128
		0.3					119*
			72	8	294	137
			86	10	260	101
		0.4


Positive Control	3-Methylcholanthrene	2.5	78	21	560	241	242*
			89	44	611	229
			82#	35	630	257

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination