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Study Information
Study Attribute Value
CEBS Accession Number: 002-02945-0024-0000-8
Chemical Name: Trichloroethylene
CASRN: 79-01-6
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 623351
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 112 117 60 18 19
    105 76 73 23
    100# 107 45 15
Test Chemical Trichloroethylene 
 0.0078    
 80# 87 61.5 26 24
    91 72 71 26
    86 75 54 21
  
 0.0156    
 112# 109 55.5 17 17
    89 100 36 13
    84# 70 55.5 22
  
 0.0313    
 78 108 35 15 19
    64# 60 43 22
  
 0.0625    
 102 96 49 16 16
    85 105 38 15
    83# 69 43.5 18
  
 0.125     
 82 97 50 20 13
    98 87 26 9
    92 92 28 10
  
 0.25      
 97 66 45 15 15
    104 72 39 12
    102 47 51 17
  
 0.5       
   
   
Positive Control Ethyl Methane Sulfonate 
250         
 65# 64 796.5 411 388*
    93 64 1018 366

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 105 100 94 30 24
    94 98 74 26
    115 110 68 20
    112 92 68 20
Test Chemical Trichloroethylene 
 0.025     
 103 95 69 22 23
    99 86 84 28
    95 89 56 20
  
 0.05      
 111 73 83 25 23
    92 66 74 27
    95 93 49 17
  
 0.1       
 100 62 46 15 17
    97 81 53 18
    124r 66 107 29
  
 0.2       
 80 17 58 24 23
    105 45 72 23
    90 56 63 23
  
 0.3       
 105 23 53 17 22
   
    94 20 78 28
  
 0.4       
   
   
Positive Control Ethyl Methane Sulfonate 
250         
 61# 40 957 524 549*
    68 34 1188 584
    69# 46 1117.5 540

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 111 113 74 22 23
    135r 67 59 15
    120r 103 49 14
    90# 87 63 23
Test Chemical Trichloroethylene 
 0.0156    
 98 68 69 24 27
    93 91 70 25
    69# 64 66 32
  
 0.0313    
 105# 83 82 26 24
    113# 101 81 24
    99 60 62 21
  
 0.0625    
 97 75 78 27 23
    91 66 64 23
    111# 84 59 18
  
 0.125     
 86# 43 99 38 30
    112 43 115 34
    107 68 58 18
  
 0.25      
 103 38 113 37 39*
    88# 15 117 44
    103 23 113 37
Positive Control 3-Methylcholanthrene 
2.5       
 101 33 444 147 179*
    90 40 474 176
    76 39 485 214

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 106# 81 261 82 71
    113 102 233 69
    93 114 178 64
    107 102 227 71
Test Chemical Trichloroethylene 
 0.025     
 99 66 331 112 101
    105 74 308 98
    96 74 265 92
  
 0.05      
 106 56 342 108 97
    108 61 311 96
    108 60 284 88
  
 0.1       
 90 48 403 150 128*
    82 39 257 105
    100 39 382 128
  
 0.2       
 94 20 448 158 160*
    100 16 441 147
    95 17 498 175
  
 0.3       
 169*
    88 10 382 145
    74 8 427 192
  
 0.4       
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 80 22 775 325 315*
    91 45 813 299
    83# 35 802.5 322

Induced S9 Trial: 3 Experiment Call:
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 104# 82 175.5 56 49
    112 103 157 47
    92 114 131 48
    105 102 150 48
Test Chemical Trichloroethylene 
 0.025     
 98 66 206 70 65
    94 73 168 59
    104 74 199 64
  
 0.05      
 107 60 202 63 65
    107 61 201 63
    104 56 219 70
  
 0.1       
 80 38 169 71 87*
    99 39 255 86
    88 48 271 103
  
 0.2       
 99 16 282 95 110*
    93 20 295 106
    85 16 328 128
  
 0.3       
 119*
    72 8 294 137
    86 10 260 101
  
 0.4       
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 78 21 560 241 242*
    89 44 611 229
    82# 35 630 257

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination