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Study Information
Study Attribute Value
CEBS Accession Number: 002-02996-0001-0000-9
Chemical Name: Vat blue 4
CASRN: 81-77-6
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 143331_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 06/10/1988    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1035 449 0.434 8.98 26.0 0.000
Test Chemical Vat blue 4 
1.6       
 50 1035 553 0.534 11.06 26.0 23.163 
5         
 50 1033 558 0.540 11.16 26.0 24.517 
16         
 50 1040 599 0.576 11.98 26.0 32.766 
50         
 50 1035 566 0.547 11.32 26.0 26.058
Positive Control Mitomycin-C 
 0.0E-4    
 50 1030 556 0.540 11.12 26.0 24.432
Mitomycin-C 
 0.005     
 10 208 296 1.423 29.60 26.0 228.037
Trend: 3.758        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 07/19/1988    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1047 368 0.351 7.36 26.0 0.000
Test Chemical Vat blue 4 
1.6       
 50 1047 512 0.489 10.24 26.0 39.130 
5         
 50 1048 507 0.484 10.14 26.0 37.640 
16         
 50 1049 572 0.545 11.44 26.0 55.138 
50         
 50 1050 516 0.491 10.32 26.0 39.817
Positive Control Mitomycin-C 
 0.0E-4    
 50 1049 517 0.493 10.34 26.0 40.221
Mitomycin-C 
 0.005     
 10 210 245 1.167 24.50 26.0 231.929
Trend: 5.009        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 05/10/1988    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1048 368 0.351 7.36 26.0 0.000
Test Chemical Vat blue 4 
1.6       
 50 1050 482 0.459 9.64 26.0 30.729 
5         
 50 1050 426 0.406 8.52 26.0 15.540 
16         
 50 1048 376 0.359 7.52 26.0 2.174 
50         
 50 1049 486 0.463 9.72 26.0 31.939
Positive Control Cyclophosphamide 
 0.1       
 50 1048 500 0.477 10.00 26.0 35.870
Cyclophosphamide 
 0.6       
 10 210 196 0.933 19.60 26.0 165.797
Trend: 1.982        
Probability: 0.024