Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-03003-0008-0000-6
Chemical Name:	Vinyl toluene
CASRN:	25013-15-4
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	327329
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	72	95	112	52	43
			98	138	71	24
			99	89	117	40
			52	79	87	55
Test Chemical	Vinyl toluene	12.5	53	84	75	47	54
			55	58	101	61	54
		25	67	89	88	44	47
			73	88	111	51	47
		50	71	76	138	65	58
			70	62	109	52	58
		100

Positive Control	Methyl Methane Sulfonate	15	23	17	593	853	708*
Positive Control			31	27	526	563	708*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	65	100	131	68	70
			70	94	125	60
			67	110	145	72
			60	96	149	82
Test Chemical	Vinyl toluene	10	149r	201	154	34	62
			57	91	111	65
			73	109	130	59
		20	75	60	136	61	66
			66	72	121	61
			64	66	147	77
		40	61	26	206	112	102
			59	45	171	97
			62	33	180	97
		60					311*
			40	5	419	351
			51	6	415	271
		80


Positive Control	Methyl Methane Sulfonate	15	25	20	247	329	378*
Positive Control			21	19	263	428	378*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	59	87	88	50	54
			48r	88	64	45
			62	103	99	54
			84	110	145	58
Test Chemical	Vinyl toluene	40	70	74	38	18	28
			49	51	59	40
			56	70	45	27
		45	57	54	62	36	33
			54	47	46	29
			75	66	80	35
		50	61	31	61	34	31
			59	41	48	27
			77	35	76	33
		55	92	24	103	38	38
			60	38	79	44
			56	26	55	33
		60	49	9	280	192	147*
			66	9	309	156
			48	6	132	91
		65


Positive Control	Methyl Methane Sulfonate	15	24	20	145	204	283*
Positive Control			17	14	185	363	283*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination