Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-03004-0020-0000-1
Chemical Name:	Vinylidene chloride
CASRN:	75-35-4
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	953885
Study Result:	Positive

View Study Details | View Protocol | Download To Excel

Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. %	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Air	0	62	117	57	31	22
			81	101	41	17
			63	103	48	26
			52	80	20	13
Test Chemical	Vinylidene chloride	1	78	127	72	31	24
			71#	134	37.5	18	24
		2	65	108	64	33
			0r	0	58	0
		4	69	91	81	39	37*
			69	98	70	34	37*
		6	70	117	53	25	29
			58	92	59	34	29
		8	71	107	74	35	34
			76	128	76	33	34
Positive Control	Methyl Methane Sulfonate	15	24	23	57	81	87*
Positive Control			27#	27	75	93	87*

Nonactivation Trial: 2 Experiment Call: Inconclusive
		Conc. %	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Air	0	79	109	68	29	25
			76	93	62	27
			78	99	40	17
			85	100	63	25
Test Chemical	Vinylidene chloride	3	68	94	32	16	20
			65	79	46	24	20
		6	74	92	39	18	15
			72	101	28	13	15
		9	67	98	31	15	16
			61	88	30	16	16
		12	66	91	22	11	12
			68	83	28	14	12
		15	67	59	27	13	16
			71	74	40	19	16
Positive Control	Methyl Methane Sulfonate	15	35	21	159	152	155*
Positive Control			33	21	154	158	155*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. %	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Air	0	79	89	52	22	32
			71	78	133	62
			79	103	66	28
			73	130	31	14
Test Chemical	Vinylidene chloride	10	60	77	61	34	41
			63	88	92	49	41
		15	82	69	66	27	30
			60	60	60	33	30
		20	65	94	47	24	33
			56	81	70	42	33
		25	64	93	60	31	30
			63	66	53	28	30
		30	43	22	63	49	59*
			32	19	66	69	59*
Positive Control	Methyl Methane Sulfonate	15	27	18	202	252	267*
Positive Control			16	18	137	282	267*

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. %	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Air	0	73	100	107	49	46
			68	88	92	45
			77	115	94	41
			67	97	97	48
Test Chemical	Vinylidene chloride	0.16	68	69	201	99	92*
			81	70	207	86	92*
		0.31	46	42	210	153	127*
			70	79	213	102	127*
		0.63	47	37	299	211	200*
			52	37	292	188	200*
		1.25	31	22	308	337	304*
			36	25	294	271	304*
		2.5	45	22	305	227	258*
			41	16	359	290	258*
Positive Control	3-Methylcholanthrene	2.5	33	20	393	399	427*
Positive Control			34	15	458	456	427*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. %	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Air	0	79	127	124	52	56
			60	86	118	66
			81	87	115	48
			83#	100	139.5	56
Test Chemical	Vinylidene chloride	1	57	25	293	171	204*
			35	13	251	237	204*
		1.5	44	12	297	227	238*
			23	6	174	249	238*
		2	46	11	264	193	211*
			33	10	226	228	211*
		2.5	43	13	251	195	200*
			22	8	134	205	200*
		3.5	8r	1	184	783
			4r	1	87	757
Positive Control	3-Methylcholanthrene	2.5	28	11	422	502	600*
Positive Control			24	9	495	697	600*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination