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Study Information
Study Attribute Value
CEBS Accession Number: 002-03007-0014-0000-7
Chemical Name: Water
CASRN: 7732-18-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 155457
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 88 123 107 40 40
    102 99 103 34
    112 85 150 45
    80 93 99 41
Test Chemical Water 
 0.5       
 108 94 96 30 44
    81# 91 150 62
    79 102 98 41
  
1         
 92 94 98 36 37
    120 108 116 32
    105 102 133 42
  
2         
 104 94 141 45 43
    107 125 108 34
    71 88 104 49
  
3         
 83 117 104 42 42
    98 101 119 41
    83 91 112 45
  
4         
 92 100 94 34 38
    84 104 95 38
    87 100 114 44
  
5         
 90 85 118 44 41
    92 112 87 32
    105 86 147 46
Positive Control Methyl Methane Sulfonate 
5         
 79 65 504 214 206*
    86 55 603 234
    104 79 528 170

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 91 94 119 44 43
    104 112 130 42
    88 113 122 46
    65 81 81 41
Test Chemical Water 
 0.5       
 69 88 85 41 50
    55 63 87 53
    67 73 110 55
  
1         
 84 75 95 38 37
    80 91 71 30
    74 79 99 45
  
3         
 75 44 111 49 50
    65 18 134 69
    99 132 95 32
  
4         
 81 97 142 58 45
    92 86 88 32
    81 107 111 46
  
5         
 81 108 75 31 38
    71 70 88 41
    68 111 87 43
Positive Control Methyl Methane Sulfonate 
5         
 42 24 506 403 398*
    53 44 582 367
    49 44 627 425

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 86# 120 96 37 49
    86# 94 154 60
    78# 86 119 51
Test Chemical Water 
 0.5       
 97# 109 108 37 47
    73# 103 98 45
    80 97 139 58
  
1         
 77# 101 111 48 42
    93 98 108 39
    62# 86 71 38
  
2         
 101 56 150 49 44
    92# 85 109 40
  
3         
 95# 109 115 40
  
4         
 67# 67 136 67 58
    70# 88 106 50
    80# 85 137 57
  
5         
 88# 105 154 58 55
    87 118 137 53
Positive Control 3-Methylcholanthrene 
2.5       
 24 6 566 781 995*
    11# 1 399 1209

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 80 128 73 30 32
    67 107 57 28
    64# 55 66 34
    69 110 68 33
Test Chemical Water 
 0.5       
 77 110 107 46 42
    82 115 116 47
    76 100 74 32
  
1         
 73 103 71 32 40
    63 101 74 39
    76# 96 108 47
  
2         
 75 103 93 42 38
    63 76 70 37
    79 119 85 36
  
3         
 89 90 111 42 33
    87 121 82 31
    87 107 67 26
  
4         
 95 75 82 29 37
    86 117 104 40
    74 122 91 41
  
5         
 71 127 67 32 37
    58 92 80 46
    89# 133 91 34
Positive Control 3-Methylcholanthrene 
2.5       
 43 12 782 613 660*
    29# 10 613 705
    35 13 698 662

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination