Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-03010-0011-0000-8
Chemical Name:	Xylenes (mixed)
CASRN:	1330-20-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	196823
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	95	104	84	30	23
			94	89	62	22
			97	97	65	22
			98	110	53	18
Test Chemical	Xylenes (mixed)	16	99	87	69	23	22
			98	82	60	20	22
		24	95	87	57	20	21
			111	111	70	21	21
		32	80	59	38	16	20
			71	84	51	24	20
		40	72	56	53	24	29
			76	70	77	34	29
		48	73	73	63	29	26
			65	70	44	23	26
Positive Control	Methyl Methane Sulfonate	15	57	32	244	144	164*
Positive Control			47	42	261	184	164*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	68	91	43	21	17
			82	108	41	17
			89	99	36	14
			73	102	36	17
Test Chemical	Xylenes (mixed)	45	80	83	45	19	18
			87	94	45	17	18
		60	61	67	47	26	24
			61	34	40	22	24
		75	80	53	71	30	35*
			55	46	67	40	35*
		90	81	38	53	22	25
			87	19	74	28	25
		105	49	7	66	45	45*
			33	5	44	44	45*
Positive Control	Methyl Methane Sulfonate	15	30	22	92	103	108*
Positive Control			25	19	85	113	108*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	77	89	86	37	33
			94	86	98	35
			89	137	77	29
			91	89	79	29
Test Chemical	Xylenes (mixed)	45	83	98	85	34	32
			95	108	85	30	32
		60	92	93	114	42	30
			87	105	48	18	30
		75	93	75	84	30	27
			82	83	57	23	27
		90	69	52	47	23	34
			64	39	86	45	34
		105	59	15	142	80	88*
			67	11	191	95	88*
Positive Control	Methyl Methane Sulfonate	15	39	35	162	138	132*
Positive Control			35	29	130	126	132*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination