Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-03013-0009-0000-8
Chemical Name:	Zearalenone
CASRN:	17924-92-4
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	669509
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	88	136	55	21	29
			80	33	89	37
			97	125	89	30
			79	106	65	27
Test Chemical	Zearalenone	7.5	73	98	53	24	33
			67	88	86	43	33
		15	60	72	82	45	43
			66	71	82	41	43
		30	74	70	96	43	48*
			80	79	126	53	48*
		60	65	12	159	82	86*
			49	7	131	90	86*
Positive Control	Methyl Methane Sulfonate	15	53	41	205	129	138*
			39	38	172	148	138*
	Methyl Methane Sulfonate	250	72	89	388	180	183*
			68	93	379	186	183*

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	83	94	77	31	28
			83	108	62	25
			80	102	76	32
			74	96	55	25
Test Chemical	Zearalenone	10	87	67	66	25	25
			94	59	68	24	25
		20	85	73	56	22	20
			88	73	49	19	20
		40	98	58	68	23	25
			91	67	72	26	25
		60

Positive Control	Methyl Methane Sulfonate	15	41	27	161	130	126*
			46	27	170	123	126*
	Methyl Methane Sulfonate	250	79	77	322	136	134*
			79	82	311	131	134*

Nonactivation Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	95	90	94	33	29
			84#	90	69	27
			86	105	65	25
			89	115	77	29
Test Chemical	Zearalenone	15	74	60	56	25	24
			63	43	44	23	24
		25	86	78	101	39	34
			72	62	61	28	34
		35	100	68	115	38	41
			90	46	118	44	41
		45	64	37	89	47	42
			74	45	84	38	42
		55

Positive Control	Methyl Methane Sulfonate	15	58	43	112	65	72*
			49	36	116	79	72*
	Methyl Methane Sulfonate	250	81	84	362	148	146*
			83#	88	357	143	146*

Nonactivation Trial: 4 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	78	105	131	56	56
			61	85	120	65
			76	98	108	48
			71	112	118	56
Test Chemical	Zearalenone	15	77	85	115	50	60
			66	71	137	69	60
		25	62	68	111	60	63
			51	65	103	67	63
		35	50	53	105	70	73
			53	61	120	76	73
		45	60	49	126	70	65
			62	56	112	61	65
		55	69	34	149	72	69
			69	30	140	67	69
		65

Positive Control	Methyl Methane Sulfonate	15	47	40	272	192	192*
			33	28	191	192	192*
	Methyl Methane Sulfonate	250	63	70	436	230	217*
			74	67	450	204	217*

Nonactivation Trial: 5 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	87	99	65	25	24
			81	86	58	24
			87	100	65	25
			85	115	53	21
Test Chemical	Zearalenone	40	84	51	61	24	29
			68	47	67	33	29
		45	81	49	64	26	27
			74	37	63	28	27
		50	73	44	55	25	26
			78	48	65	28	26
		55	70	22	60	29	24
			61	24	34	18	24
		60	63	9	36	19	27
			65	11	68	35	27
		65

Positive Control	Methyl Methane Sulfonate	15	38	30	204	180	167*
Positive Control			38	24	175	154	167*

Induced S9 Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	63	101	52	28	31
			77	100	53	23
			59	89	68	38
			72	111	80	37
Test Chemical	Zearalenone	40	63	45	43	23	27
			69	53	65	31	27
		45	68	47	42	21	24
			83	45	66	27	24
		50	64	33	55	29	25
			64	35	42	22	25
		55	56	33	57	34	31
			61	33	52	28	31
		60	38	12	59	52	46
			53	14	64	40	46
		65

Positive Control	3-Methylcholanthrene	2.5	31	15	212	232	221*
Positive Control			46	17	292	211	221*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	74	82	157	71	63
			99	108	167	56
			111	103	235	71
			113	107	188	55
Test Chemical	Zearalenone	45	76	57	221	97	99*
			63	56	191	101	99*
		50	68	51	259	127	126*
			64	54	236	124	126*
		55	68	48	244	120	123*
			73	42	278	126	123*
		60	74	30	314	142	152*
			104	28	508	163	152*
		65	79	27	199	84	84
			95	21	242	85	84
Positive Control	3-Methylcholanthrene	2.5	68	38	651	318	308*
Positive Control			66	37	589	297	308*

Induced S9 Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	93	104	47	17	25
			82	87	78	32
			80	93	59	24
			85	116	71	28
Test Chemical	Zearalenone	40	60	62	60	34	29
			69	68	51	25	29
		45	47	48	5	4	19
			70	63	72	34	19
		50	72	49	64	30	27
			81	50	61	25	27
		55	50	37	43	29	32
			65	36	70	36	32
		60	72	24	70	32	29
			73	25	55	25	29
		65

Positive Control	3-Methylcholanthrene	2.5	42	20	324	258	258*
Positive Control			42	23	320	257	258*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination