Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-03018-0010-0000-5
Chemical Name:	Ziram
CASRN:	137-30-4
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	746976
Study Result:	Positive

View Study Details | View Protocol | Download To Excel

Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	95	102	138	48	50
			94	89	141	50
			85	98	123	48
			86	110	135	53
Test Chemical	Ziram	0.0625	67	19	270	135	161*
			67	22	374	187	161*
		0.125	68	22	251	122	114*
			64	21	201	105	114*
		0.25	57	20	193	114	111*
			68	25	218	108	111*
		0.5	54	24	176	108	112*
			65	23	227	116	112*
		1	41	19	156	126	118*
			59	17	196	110	118*
		2

Positive Control	Methyl Methane Sulfonate	15	36	21	248	228	303*
			26	15	295	378	303*
	Methyl Methane Sulfonate	250	45	41	451	332	333*
			37	37	372	334	333*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	77	101	70	30	27
			84	86	61	24
			94	95	78	28
			102	118	73	24
Test Chemical	Ziram	0.1	99	46	276	93	104*
			99	40	339	115	104*
		0.2	97	47	243	84	90*
			96	43	279	97	90*
		0.6	68	33	233	114	110*
			89	40	284	107	110*
		1	76	26	245	108	108*
			89	32	288	107	108*
		1.4	57	7	311	183	186*
			68	9	385	188	186*
		1.8

Positive Control	Methyl Methane Sulfonate	15	38	30	196	171	153*
			36	27	145	135	153*
	Methyl Methane Sulfonate	250	89	81	465	175	174*
			70	70	364	173	174*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination