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Study Information
Study Attribute Value
CEBS Accession Number: 002-03050-0014-0000-5
Chemical Name: bis(2-Chloro-1-methylethyl) ether
CASRN: 108-60-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 151175
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 69 97 87 42 39
    66 97 73 37
    76 95 94 41
    61 110 64 35
Test Chemical bis(2-Chloro-1-methylethyl) ether 
62.5       
 57 80 96 56 58
    66 91 116 59
  
125         
 57 70 107 62 58
    62 73 101 54
  
250         
 56 51 172 103 92*
    64 60 154 81
  
500         
 72 47 211 98 84*
    58 45 123 70
  
1000         
 31 4 1315 1406 1148*
    53 8 1424 890
  
2000         
   
Positive Control Methyl Methane Sulfonate 
15         
 45 27 277 204 245*
    34 22 291 287

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 121r 92 224 62 74
    85 99 195 76
    89 97 202 76
    79 103 164 69
Test Chemical bis(2-Chloro-1-methylethyl) ether 
50         
 108 87 154 47 49
    103 91 157 51
  
250         
 77 81 121 52 54
    99 80 165 56
  
500         
 101 67 278 91 74
    101 68 173 57
  
750         
 67 28 1189 596 558*
    72 29 1129 520
  
1000         
   
Positive Control Methyl Methane Sulfonate 
15         
 49 28 158 109 102
    42 25 121 96

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination