Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-03050-0014-0000-5
Chemical Name:	bis(2-Chloro-1-methylethyl) ether
CASRN:	108-60-1
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	151175
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	69	97	87	42	39
			66	97	73	37
			76	95	94	41
			61	110	64	35
Test Chemical	bis(2-Chloro-1-methylethyl) ether	62.5	57	80	96	56	58
			66	91	116	59	58
		125	57	70	107	62	58
			62	73	101	54	58
		250	56	51	172	103	92*
			64	60	154	81	92*
		500	72	47	211	98	84*
			58	45	123	70	84*
		1000	31	4	1315	1406	1148*
			53	8	1424	890	1148*
		2000

Positive Control	Methyl Methane Sulfonate	15	45	27	277	204	245*
Positive Control			34	22	291	287	245*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	121r	92	224	62	74
			85	99	195	76
			89	97	202	76
			79	103	164	69
Test Chemical	bis(2-Chloro-1-methylethyl) ether	50	108	87	154	47	49
			103	91	157	51	49
		250	77	81	121	52	54
			99	80	165	56	54
		500	101	67	278	91	74
			101	68	173	57	74
		750	67	28	1189	596	558*
			72	29	1129	520	558*
		1000

Positive Control	Methyl Methane Sulfonate	15	49	28	158	109	102
Positive Control			42	25	121	96	102

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination