Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-03067-0014-0000-3
Chemical Name:	m-Chloroaniline
CASRN:	108-42-9
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	435631
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	83	94	255	103	85
			94	106	216	77
			99	101	239	81
			72	98	172	79
Test Chemical	m-Chloroaniline	100	61	85	179	98	88
			70	85	165	79	88
		200	77	70	213	92	93
			76	60	213	94	93
		300	23	26	139	200	200*
			36	35	216	200	200*
		400	27	23	161	200	200*
			36	23	213	200	200*
		500	29	9	176	200	200*
			35	10	211	200	200*
Positive Control	Methyl Methane Sulfonate	15	24	19	288	400	336*
Positive Control			30	24	242	272	336*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	94	90	113	40	35
			98	94	83	28
			110	108	86	26
			97	109	131	45
Test Chemical	m-Chloroaniline	160	83	62	72	29	35
			113	76	142	42	35
		240	90	56	136	50	47
			93	41	121	43	47
		320	73	31	122	55	56*
			84	25	145	57	56*
		400	79	24	156	66	64*
			102	26	187	61	64*
		480	86	10	226	87	88*
			84	10	222	88	88*
Positive Control	Methyl Methane Sulfonate	15	55	37	250	152	151*
Positive Control			44	30	198	149	151*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	92	93	79	29	21
			94	109	44	16
			107	107	88	27
			102	90	42	14
Test Chemical	m-Chloroaniline	440	73	14	71	33	30
			70	12	58	28	30
		460	85	11	55	22	22
			74	14	48	22	22
		480	110	10	80	24	29
			78	9	77	33	29
		500	61#	8	84	46	36
			100	8	77	26	36
		520	63	6	84	45	48*
			57	6	87	51	48*
Positive Control	Methyl Methane Sulfonate	15	41	26	120	97	95*
Positive Control			42	31	117	93	95*

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	104	83	146	47	41
			90	84	110	41
			92	117	85	31
			108	115	145	45
Test Chemical	m-Chloroaniline	240	74	52	185	84	89*
			68	40	191	93	89*
		320	56	35	123	73	82*
			56	28	150	90	82*
		400	62	24	132	71	72*
			66	16	146	73	72*
		480	56	16	149	89	88*
			102	11	269	88	88*
		560	39	6	134	115	117*
			46	5	163	119	117*
Positive Control	3-Methylcholanthrene	2.5	50	31	591	391	357*
Positive Control			56	35	544	322	357*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	62	79	57	31	31
			67	88	62	31
			67	108	65	32
			61	124	56	31
Test Chemical	m-Chloroaniline	160	79	85	106	45	42
			66	98	76	38	42
		240	51	47	71	46	38
			66	74	60	30	38
		320	66	60	77	39	42
			60	45	82	45	42
		400	79	38	153	65	61*
			71	40	122	57	61*
		480	63	28	91	48	52*
			90	30	149	55	52*
		560	66	21	131	67	67*
			73	20	145	67	67*
Positive Control	3-Methylcholanthrene	2.5	47	33	350	248	295*
Positive Control			40	30	413	341	295*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination