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Study Information
Study Attribute Value
CEBS Accession Number: 002-03067-0014-0000-3
Chemical Name: m-Chloroaniline
CASRN: 108-42-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 435631
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide
 0         
83 94 255 103 85
    94 106 216 77
    99 101 239 81
    72 98 172 79
Test Chemical m-Chloroaniline
100         
61 85 179 98 88
    70 85 165 79
 
200         
77 70 213 92 93
    76 60 213 94
 
300         
23 26 139 200 200*
    36 35 216 200
 
400         
27 23 161 200 200*
    36 23 213 200
 
500         
29 9 176 200 200*
    35 10 211 200
Positive Control Methyl Methane Sulfonate
15         
24 19 288 400 336*
    30 24 242 272

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide
 0         
94 90 113 40 35
    98 94 83 28
    110 108 86 26
    97 109 131 45
Test Chemical m-Chloroaniline
160         
83 62 72 29 35
    113 76 142 42
 
240         
90 56 136 50 47
    93 41 121 43
 
320         
73 31 122 55 56*
    84 25 145 57
 
400         
79 24 156 66 64*
    102 26 187 61
 
480         
86 10 226 87 88*
    84 10 222 88
Positive Control Methyl Methane Sulfonate
15         
55 37 250 152 151*
    44 30 198 149

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide
 0         
92 93 79 29 21
    94 109 44 16
    107 107 88 27
    102 90 42 14
Test Chemical m-Chloroaniline
440         
73 14 71 33 30
    70 12 58 28
 
460         
85 11 55 22 22
    74 14 48 22
 
480         
110 10 80 24 29
    78 9 77 33
 
500         
61# 8 84 46 36
    100 8 77 26
 
520         
63 6 84 45 48*
    57 6 87 51
Positive Control Methyl Methane Sulfonate
15         
41 26 120 97 95*
    42 31 117 93

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide
 0         
104 83 146 47 41
    90 84 110 41
    92 117 85 31
    108 115 145 45
Test Chemical m-Chloroaniline
240         
74 52 185 84 89*
    68 40 191 93
 
320         
56 35 123 73 82*
    56 28 150 90
 
400         
62 24 132 71 72*
    66 16 146 73
 
480         
56 16 149 89 88*
    102 11 269 88
 
560         
39 6 134 115 117*
    46 5 163 119
Positive Control 3-Methylcholanthrene
2.5       
50 31 591 391 357*
    56 35 544 322

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide
 0         
62 79 57 31 31
    67 88 62 31
    67 108 65 32
    61 124 56 31
Test Chemical m-Chloroaniline
160         
79 85 106 45 42
    66 98 76 38
 
240         
51 47 71 46 38
    66 74 60 30
 
320         
66 60 77 39 42
    60 45 82 45
 
400         
79 38 153 65 61*
    71 40 122 57
 
480         
63 28 91 48 52*
    90 30 149 55
 
560         
66 21 131 67 67*
    73 20 145 67
Positive Control 3-Methylcholanthrene
2.5       
47 33 350 248 295*
    40 30 413 341

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination