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Study Information
Study Attribute Value
CEBS Accession Number: 002-03083-0001-0000-7
Chemical Name: m-Phenylenediamine
CASRN: 108-45-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 434853_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 11/06/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1015 397 0.391 7.94 25.5 0.000
Test Chemical m-Phenylenediamine 
5         
 50 1013 471 0.465 9.42 25.5 18.874 
16.7       
 50 1009 897 0.889 17.94 30.1 127.288 
50         
 50 1002 761 0.759 15.22 30.1 94.175 
166.7       
 0 0 0 0.000 0.00 30.1 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1019 520 0.510 10.40 25.5 30.468
Mitomycin-C 
 0.01      
 5 103 184 1.786 36.80 25.5 356.726
Trend: 13.910        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 11/19/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 25 498 201 0.404 8.04 25.6 0.000
Test Chemical m-Phenylenediamine 
49.8       
 25 506 428 0.846 17.12 31.7 109.569 
75.4       
 25 505 499 0.988 19.96 31.7 144.817 
100.5       
 25 502 551 1.098 22.04 31.7 171.945 
150         
 0 0 0 0.000 0.00 31.7 N/A
Positive Control Mitomycin-C 
 0.001     
 25 505 280 0.554 11.20 25.6 37.373
Mitomycin-C 
 0.01      
 5 102 205 2.010 41.00 25.6 397.951
Trend: 12.263        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 11/06/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 995 373 0.375 7.46 25.5 0.000
Test Chemical m-Phenylenediamine 
500         
 50 996 403 0.405 8.06 25.5 7.934 
1666.7       
 50 1000 476 0.476 9.52 25.5 26.976 
5000         
 50 999 518 0.519 10.36 25.5 38.318
Positive Control Cyclophosphamide 
 0.4       
 50 1021 698 0.684 13.96 25.5 82.366
Cyclophosphamide 
2         
 5 101 169 1.673 33.80 25.5 346.354
Trend: 5.332        
Probability: 0.000