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Study Information
Study Attribute Value
CEBS Accession Number: 002-03090-0002-0000-6
Chemical Name: n-Butyl acrylate
CASRN: 141-32-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 679128_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
Induced Rat Liver S9  1_S9  Questionable 
No Activation  2  Weakly Positive* 
Induced Rat Liver S9  2_S9  Questionable 
No Activation  3  Test Failure 
Induced Rat Liver S9  3_S9  Test Failure 

Trial #:1   Activation: No Activation    Date: 10/15/1986    Harvest Time: 20 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 5 0.025 2.5 1 0.005 0.5 3 0.015 1.5 1 0.005 0.5
Dimethyl Sulfoxide 
0         
 200 10 0.050 4.5 1 0.005 0.5 5 0.025 2.5 4 0.020 2.0
Test Chemical: n-Butyl acrylate 
17.1       
 100 83 0.830 43.0 42 0.420 31.0 30 0.300 19.0 11 0.110 2.0 
25.1       
 25 40 1.600 48.0 7 0.280 24.0 13 0.520 32.0 20 0.800 8.0 
32.2       
 10 47 4.700 100.0 7 0.700 60.0 30 3.000 80.0 10 1.000 10.0 
40.3       
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.05      
 200 120 0.600 37.0 44 0.220 20.0 55 0.275 22.0 21 0.105 1.5
Mitomycin-C 
 0.08      
 25 45 1.800 68.0 11 0.440 36.0 24 0.960 44.0 10 0.400 4.0
Trend: 9.675 7.638 8.238  
Probability: 0.000 0.000 0.000  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 10/15/1986    Harvest Time: 12 hour(s)   Trial Call: Questionable   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 11 0.055 5.0 5 0.025 2.5 3 0.015 1.0 3 0.015 1.5
Dimethyl Sulfoxide 
0         
 200 7 0.035 2.5 3 0.015 1.5 2 0.010 0.5 2 0.010 1.0
Test Chemical: n-Butyl acrylate 
200.6       
 200 15 0.075 6.5 2 0.010 1.0 11 0.055 4.5 2 0.010 1.0 
250.8       
 200 10 0.050 4.5 3 0.015 1.5 6 0.030 2.5 1 0.005 0.5 
298.6       
 200 21 0.105 8.5 4 0.020 2.0 14 0.070 5.0 3 0.015 1.5 
398.1       
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
7.5       
 200 41 0.205 13.5 6 0.030 2.5 23 0.115 9.5 12 0.060 1.5
Cyclophosphamide 
37.5       
 25 32 1.280 60.0 9 0.360 24.0 13 0.520 44.0 10 0.400 4.0
Trend: 2.181 0.491 2.082  
Probability: 0.015 0.312 0.019  


Trial #:2   Activation: No Activation    Date: 08/20/1986    Harvest Time: 20.2 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 8 0.040 4.0 4 0.020 2.0 2 0.010 1.0 2 0.010 1.0
Dimethyl Sulfoxide 
0         
 200 7 0.035 3.5 3 0.015 1.5 1 0.005 0.5 3 0.015 1.5
Test Chemical: n-Butyl acrylate 
7.5       
 200 13 0.065 5.5 5 0.025 2.0 6 0.030 3.0 2 0.010 1.0 
10.1       
 200 27 0.135 7.5 5 0.025 2.5 12 0.060 5.0 10 0.050 0.5 
25.3       
 10 32 3.200 70.0 12 1.200 40.0 20 2.000 70.0 0 0.000 0.0 
37.7       
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.05      
 200 97 0.485 25.0 20 0.100 10.0 53 0.265 18.0 24 0.120 3.0
Mitomycin-C 
 0.08      
 25 15 0.600 28.0 8 0.320 20.0 6 0.240 16.0 1 0.040 4.0
Trend: 4.972 3.782 6.767  
Probability: 0.000 0.000 0.000  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 08/20/1986    Harvest Time: 12 hour(s)   Trial Call: Questionable   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 19 0.095 7.5 7 0.035 3.5 9 0.045 3.5 3 0.015 1.5
Dimethyl Sulfoxide 
0         
 200 9 0.045 4.5 2 0.010 1.0 5 0.025 2.5 2 0.010 1.0
Test Chemical: n-Butyl acrylate 
150.8       
 200 21 0.105 9.5 6 0.030 3.0 6 0.030 2.5 9 0.045 4.5 
225         
 200 18 0.090 8.0 7 0.035 3.5 10 0.050 4.0 1 0.005 0.5 
300         
 200 22 0.110 10.0 6 0.030 3.0 15 0.075 6.5 1 0.005 0.5
Positive Control: Cyclophosphamide 
7.5       
 200 55 0.275 20.5 15 0.075 6.5 27 0.135 13.0 13 0.065 2.0
Cyclophosphamide 
37.5       
 25 13 0.520 44.0 5 0.200 16.0 8 0.320 32.0 0 0.000 0.0
Trend: 1.717 1.318 2.179  
Probability: 0.043 0.094 0.015  


Trial #:3   Activation: No Activation    Date: 07/23/1986    Harvest Time: 20.3 hour(s)   Trial Call: Test Failure   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 17 0.085 8.5 10 0.050 5.0 4 0.020 2.0 3 0.015 1.5
Dimethyl Sulfoxide 
0         
 200 15 0.075 7.0 9 0.045 4.5 4 0.020 2.0 2 0.010 1.0
Test Chemical: n-Butyl acrylate 
5         
 200 18 0.090 7.0 4 0.020 2.0 11 0.055 3.5 3 0.015 1.5 
7.5       
 200 27 0.135 10.5 13 0.065 6.5 13 0.065 5.0 1 0.005 0.5 
10.1       
 50 65 1.300 48.0 9 0.180 16.0 36 0.720 36.0 20 0.400 4.0 
25.2       
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.05      
 200 54 0.270 23.0 26 0.130 13.0 28 0.140 12.0 0 0.000 0.0
Mitomycin-C 
 0.08      
 25 17 0.680 52.0 6 0.240 24.0 11 0.440 36.0 0 0.000 0.0
Trend: 4.976 2.340 5.501  
Probability: 0.000 0.010 0.000  


Trial #:3_S9   Activation: Induced Rat Liver S9    Date: 07/24/1986    Harvest Time: 12 hour(s)   Trial Call: Test Failure   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 34 0.170 14.0 7 0.035 3.5 16 0.080 6.5 11 0.055 5.5
Dimethyl Sulfoxide 
0         
 200 25 0.125 12.0 11 0.055 5.5 9 0.045 4.5 5 0.025 2.5
Test Chemical: n-Butyl acrylate 
66.2       
 200 32 0.160 12.5 5 0.025 2.5 18 0.090 6.5 9 0.045 4.5 
132.3       
 200 25 0.125 9.5 12 0.060 5.0 10 0.050 3.5 3 0.015 1.5 
200.4       
 200 18 0.090 7.0 7 0.035 3.5 10 0.050 3.0 1 0.005 0.5 
267.2       
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
7.5       
 200 32 0.160 16.0 12 0.060 6.0 16 0.080 8.0 4 0.020 2.0
Cyclophosphamide 
37.5       
 25 6 0.240 24.0 2 0.080 8.0 4 0.160 16.0 0 0.000 0.0
Trend: -1.893 -0.425 -1.244  
Probability: 0.971 0.664 0.893