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Study Information
Study Attribute Value
CEBS Accession Number: 002-03091-0001-0000-6
Chemical Name: n-Butyl chloride
CASRN: 109-69-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 297558_SCE
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
No Activation  2  Negative 
Induced Rat Liver S9  1_S9  Negative 
Induced Rat Liver S9  2_S9  Test Failure 

Trial #: 1    Activation: No Activation    Date: 02/06/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1042 359 0.345 7.18 26.0 0.000
Negative (Not Specified) 
0         
 50 1044 392 0.375 7.84 26.0 8.983
Test Chemical n-Butyl chloride 
160         
 50 1043 394 0.378 7.88 26.0 9.644 
500         
 50 1050 362 0.345 7.24 26.0 0.067 
1600         
 50 1044 393 0.376 7.86 26.0 9.261 
5000         
 50 1042 410 0.393 8.20 26.0 14.206
Positive Control Mitomycin-C 
 0.001     
 50 1042 970 0.931 19.40 26.0 170.195
Mitomycin-C 
 0.01      
 10 209 465 2.225 46.50 26.0 545.773
Trend: 1.629        
Probability: 0.052        

Trial #: 2    Activation: No Activation    Date: 03/21/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1044 394 0.377 7.88 26.0 -7.529
Dimethyl Sulfoxide 
0         
 50 1034 422 0.408 8.44 26.0 0.000
Test Chemical n-Butyl chloride 
500         
 50 1037 402 0.388 8.04 26.0 -5.015 
1600         
 30 626 279 0.446 9.30 26.0 9.204 
3000         
 50 1040 396 0.381 7.92 26.0 -6.703 
4000         
 50 1042 419 0.402 8.38 26.0 -1.473 
5000         
 50 1042 453 0.435 9.06 26.0 6.522
Positive Control Mitomycin-C 
 0.001     
 50 1039 1303 1.254 26.06 26.0 207.282
Mitomycin-C 
 0.01      
 10 204 510 2.500 51.00 26.0 512.559
Trend: 0.602        
Probability: 0.274        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 02/07/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1047 469 0.448 9.38 26.0 1.940
Dimethyl Sulfoxide 
0         
 50 1040 457 0.439 9.14 26.0 0.000
Test Chemical n-Butyl chloride 
160         
 50 1048 423 0.404 8.46 26.0 -8.146 
500         
 50 1044 439 0.420 8.78 26.0 -4.307 
1600         
 50 1046 347 0.332 6.94 26.0 -24.506 
5000         
 50 1039 471 0.453 9.42 26.0 3.163
Positive Control Cyclophosphamide 
 0.3       
 50 1037 571 0.551 11.42 26.0 25.307
Cyclophosphamide 
2         
 50 1039 1384 1.332 27.68 26.0 203.136
Trend: -0.719        
Probability: 0.764        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 03/21/1984    Trial Call: Test Failure   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1040 449 0.432 8.98 26.0 1.939
Dimethyl Sulfoxide 
0         
 50 1046 443 0.424 8.86 26.0 0.000
Test Chemical n-Butyl chloride 
1600         
 50 1048 457 0.436 9.14 26.0 2.963 
3000         
 50 1048 449 0.428 8.98 26.0 1.161 
4000         
 50 1046 484 0.463 9.68 26.0 9.255 
5000         
 50 1048 482 0.460 9.64 26.0 8.596
Positive Control Cyclophosphamide 
 0.3       
 50 1047 527 0.503 10.54 26.0 18.848
Cyclophosphamide 
2         
 10 211 256 1.213 25.60 26.0 186.474
Trend: 1.439        
Probability: 0.075