COVID-19 is an emerging, rapidly evolving situation.

Get the latest public health information from CDC and research information from NIH.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Share This:
Study Information
Study Attribute Value
CEBS Accession Number: 002-03091-0002-0000-7
Chemical Name: n-Butyl chloride
CASRN: 109-69-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 297558_CA
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Negative 
No Activation  2  Negative 
Induced Rat Liver S9  2_S9  Negative 

Trial #:1   Activation: No Activation    Date: 02/10/1984    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0
Test Chemical: n-Butyl chloride 
160         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
500         
 100 3 0.030 3.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0 
1600         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0 
5000         
 100 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0
Positive Control: Mitomycin-C 
 0.25      
 100 37 0.370 27.0 15 0.150 14.0 22 0.220 16.0 0 0.000 0.0
Mitomycin-C 
1         
 50 24 0.480 44.0 6 0.120 12.0 18 0.360 32.0 0 0.000 0.0
Trend: -0.217 -0.322 -0.633  
Probability: 0.586 0.626 0.737  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 02/09/1984    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 4 0.040 3.0 3 0.030 3.0 1 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0
Test Chemical: n-Butyl chloride 
160         
 100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0 
500         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 
1600         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
5000         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
15         
 100 27 0.270 19.0 17 0.170 13.0 10 0.100 8.0 0 0.000 0.0
Cyclophosphamide 
50         
 50 19 0.380 28.0 12 0.240 16.0 7 0.140 14.0 0 0.000 0.0
Trend: -0.863 -0.409 -0.757  
Probability: 0.806 0.659 0.776  


Trial #:2   Activation: No Activation    Date: 03/29/1984    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: n-Butyl chloride 
1600         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
3000         
 100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0 
4000         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
5000         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.25      
 100 21 0.210 16.0 11 0.110 10.0 10 0.100 9.0 0 0.000 0.0
Mitomycin-C 
1         
 50 24 0.480 38.0 11 0.220 20.0 13 0.260 20.0 0 0.000 0.0
Trend: 0.225 0.802 -0.087  
Probability: 0.411 0.211 0.535  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 03/29/1984    Harvest Time: 12.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 3 0.030 2.0 3 0.030 2.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 5 0.050 3.0 1 0.010 1.0 4 0.040 2.0 0 0.000 0.0
Test Chemical: n-Butyl chloride 
1600         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0 
3000         
 100 4 0.040 4.0 0 0.000 0.0 4 0.040 4.0 0 0.000 0.0 
4000         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0 
5000         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
15         
 100 56 0.560 42.0 32 0.320 27.0 24 0.240 23.0 0 0.000 0.0
Cyclophosphamide 
50         
 100 87 0.870 51.0 32 0.320 26.0 55 0.550 39.0 0 0.000 0.0
Trend: -0.024 -0.480 0.172  
Probability: 0.509 0.685 0.432