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Study Information
Study Attribute Value
CEBS Accession Number: 002-03091-0011-0000-7
Chemical Name: n-Butyl chloride
CASRN: 109-69-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 048570
Study Result: Inconclusive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 92# 107 118.5 43 43
    63 98 75 40
    78# 91 94.5 40
    81 105 119 49
Test Chemical n-Butyl chloride 
31.25      
 81 94 136 56 48
    82 88 99 40
  
62.5       
 98# 87 138 47 46
    86 101 115 45
  
125         
 73 83 104 47 38
    94 72 80 28
  
250         
 95 71 95 33 32
    95 66 90 31
  
500         
    100# 11 111 37
  
1000         
   
Positive Control Methyl Methane Sulfonate 
15         
 36# 21 336 309 321*
    46 22 460 333

Nonactivation Trial: 2 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 51 99 93 61 61
    50 95 101 67
    64 116 96 50
    54 90 107 66
Test Chemical n-Butyl chloride 
50         
 58 114 101 58 53
    61 127 88 48
  
150         
 73 138 90 41 53
    49 83 95 65
  
250         
 75 101 75 33 32
    74 107 70 32
  
350         
 78# 70 170 73 76
    73 68 172 78
  
450         
    70 10 326 155
  
550         
   
Positive Control Methyl Methane Sulfonate 
15         
 14 20 213 501 486*
    27 37 377 471

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 60 96 134 74 57
    67 108 96 48
    72 111 117 54
    69 84 109 52
Test Chemical n-Butyl chloride 
350         
 66 90 113 57 54
    83 83 128 52
  
400         
 84 60 176 70 68
    94 57 184 66
  
450         
 56 44 204 121 110*
    97 50 286 99
  
500         
 83 90 255 103 249*
    65 16 769 394
  
550         
 64 13 826 430 383*
    52 24 523 335
  
600         
   
Positive Control Methyl Methane Sulfonate 
15         
 37 25 600 541 556*
    30 22 518 572

Nonactivation Trial: 4 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 77# 104 149 65 63
    70# 96 127 61
    48r 81 115 80
    41r 59 115 94
Test Chemical n-Butyl chloride 
50         
 83 85 117 47 49
    73 93 112 51
  
150         
 94 80 119 42 41
    100 78 117 39
  
250         
 68 64 132 65
  
350         
 65 59 112 58 63
    69# 63 141 68
  
450         
 51 22 111 73 66
    59 25 103 59
  
550         
   
Positive Control Methyl Methane Sulfonate 
15         
 32 23 283 295 319*
    30 19 312 343

Nonactivation Trial: 5 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 80# 107 81 34 37
    66 106 81 41
    63 90 84 44
    57 98 53 31
Test Chemical n-Butyl chloride 
300         
 83 70 85 34 23
    95 87 35 12
  
350         
 86 73 44 17 17
    86 87 41 16
  
400         
 97 62 72 25 20
    107 67 51 16
  
450         
    68 11 61 30
  
500         
   
Positive Control Methyl Methane Sulfonate 
15         
 38 35 290 252 227*
    34 30 206 201

Nonactivation Trial: 6 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 88 116 33 12 14
    111 71 34 10
    85 101 41 16
    99 112 54 18
Test Chemical n-Butyl chloride 
300         
 81 75 34 14 16
    84 60 43 17
  
350         
 87 59 17 7 11
    106 60 48 15
  
400         
 85 57 41 16 19
    89 47 60 22
  
450         
 101 35 53 17 17
    112 51 54 16
  
500         
  
550         
   
Positive Control Methyl Methane Sulfonate 
15         
 38 25 154 135 140*
    45 25 196 145

Nonactivation Trial: 7 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 94 109 50 18 20
    86 93 61 24
    83 93 50 20
    97# 105 59 20
Test Chemical n-Butyl chloride 
200         
 117 73 69 20 23
    109 74 85 26
  
250         
 68 58 53 26 26
    84 64 65 26
  
300         
 94# 45 81 29 26
    88 35 60 23
  
350         
 79 30 46 19 27
    65 30 68 35
  
400         
 54# 3 65 40 35
    75 8 68 30
  
450         
   
Positive Control Methyl Methane Sulfonate 
15         
 40# 28 220 184 173*
    57 32 275 162

Induced S9 Trial: 1 Experiment Call: Rejected
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 60 109 35 19 18
    65 96 28 14
    42r 90 20 16
    55 95 35 21
Test Chemical n-Butyl chloride 
300         
 64 88 7 4 7
    66 76 19 10
  
350         
 100 92 21 7 8
    90 113 26 10
  
400         
 70 39 8 4 6
    82 49 22 9
  
450         
 68 75 24 12 12
    83 59 31 12
  
500         
 56 78 20 12 11
    74 36 22 10
  
550         
   
Positive Control 3-Methylcholanthrene 
2.5       
 45 34 106 79 78*
    39 41 91 77

Induced S9 Trial: 2 Experiment Call: Rejected
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 76# 103 25.5 11 14
    89 106 32 12
    79 91 45 19
Test Chemical n-Butyl chloride 
250         
 75# 60 40.5 18 11
    72 75 9 4
  
300         
 65 58 21 11 10
    77 43 20 9
  
350         
 82 64 19 8 6
    85 56 13 5
  
400         
 73 37 31 14 11
    80 50 18 7
  
450         
 66 23 16 8 7
    82 42 14 6
  
500         
   
Positive Control 3-Methylcholanthrene 
2.5       
 49 33 87 60 60*
    64 41 117 61

Induced S9 Trial: 3 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 81 108 38 16 22
    75 89 41 18
    84 103 74 29
    86 100 68 26
Test Chemical n-Butyl chloride 
250         
 81 52 55 23 19
    70 64 32 15
  
300         
 76 56 53 23 22
    72 57 44 20
  
350         
 69 63 64 31 26
    74 66 45 20
  
400         
 71 54 36 17 17
    84 58 45 18
  
450         
 38 26 36 32 26
    62 29 37 20
  
500         
   
Positive Control 3-Methylcholanthrene 
2.5       
 42 18 356 283 271*
    48 27 375 260

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination