Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-03091-0011-0000-7
Chemical Name:	n-Butyl chloride
CASRN:	109-69-3
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	048570
Study Result:	Inconclusive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	92#	107	118.5	43	43
			63	98	75	40
			78#	91	94.5	40
			81	105	119	49
Test Chemical	n-Butyl chloride	31.25	81	94	136	56	48
			82	88	99	40	48
		62.5	98#	87	138	47	46
			86	101	115	45	46
		125	73	83	104	47	38
			94	72	80	28	38
		250	95	71	95	33	32
			95	66	90	31	32
		500
			100#	11	111	37
		1000

Positive Control	Methyl Methane Sulfonate	15	36#	21	336	309	321*
Positive Control			46	22	460	333	321*

Nonactivation Trial: 2 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	51	99	93	61	61
			50	95	101	67
			64	116	96	50
			54	90	107	66
Test Chemical	n-Butyl chloride	50	58	114	101	58	53
			61	127	88	48	53
		150	73	138	90	41	53
			49	83	95	65	53
		250	75	101	75	33	32
			74	107	70	32	32
		350	78#	70	170	73	76
			73	68	172	78	76
		450
			70	10	326	155
		550

Positive Control	Methyl Methane Sulfonate	15	14	20	213	501	486*
Positive Control			27	37	377	471	486*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	60	96	134	74	57
			67	108	96	48
			72	111	117	54
			69	84	109	52
Test Chemical	n-Butyl chloride	350	66	90	113	57	54
			83	83	128	52	54
		400	84	60	176	70	68
			94	57	184	66	68
		450	56	44	204	121	110*
			97	50	286	99	110*
		500	83	90	255	103	249*
			65	16	769	394	249*
		550	64	13	826	430	383*
			52	24	523	335	383*
		600

Positive Control	Methyl Methane Sulfonate	15	37	25	600	541	556*
Positive Control			30	22	518	572	556*

Nonactivation Trial: 4 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	77#	104	149	65	63
			70#	96	127	61
			48r	81	115	80
			41r	59	115	94
Test Chemical	n-Butyl chloride	50	83	85	117	47	49
			73	93	112	51	49
		150	94	80	119	42	41
			100	78	117	39	41
		250	68	64	132	65
		350	65	59	112	58	63
			69#	63	141	68	63
		450	51	22	111	73	66
			59	25	103	59	66
		550

Positive Control	Methyl Methane Sulfonate	15	32	23	283	295	319*
Positive Control			30	19	312	343	319*

Nonactivation Trial: 5 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	80#	107	81	34	37
			66	106	81	41
			63	90	84	44
			57	98	53	31
Test Chemical	n-Butyl chloride	300	83	70	85	34	23
			95	87	35	12	23
		350	86	73	44	17	17
			86	87	41	16	17
		400	97	62	72	25	20
			107	67	51	16	20
		450
			68	11	61	30
		500

Positive Control	Methyl Methane Sulfonate	15	38	35	290	252	227*
Positive Control			34	30	206	201	227*

Nonactivation Trial: 6 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	88	116	33	12	14
			111	71	34	10
			85	101	41	16
			99	112	54	18
Test Chemical	n-Butyl chloride	300	81	75	34	14	16
			84	60	43	17	16
		350	87	59	17	7	11
			106	60	48	15	11
		400	85	57	41	16	19
			89	47	60	22	19
		450	101	35	53	17	17
			112	51	54	16	17
		500
		550

Positive Control	Methyl Methane Sulfonate	15	38	25	154	135	140*
Positive Control			45	25	196	145	140*

Nonactivation Trial: 7 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	94	109	50	18	20
			86	93	61	24
			83	93	50	20
			97#	105	59	20
Test Chemical	n-Butyl chloride	200	117	73	69	20	23
			109	74	85	26	23
		250	68	58	53	26	26
			84	64	65	26	26
		300	94#	45	81	29	26
			88	35	60	23	26
		350	79	30	46	19	27
			65	30	68	35	27
		400	54#	3	65	40	35
			75	8	68	30	35
		450

Positive Control	Methyl Methane Sulfonate	15	40#	28	220	184	173*
Positive Control			57	32	275	162	173*

Induced S9 Trial: 1 Experiment Call: Rejected
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	60	109	35	19	18
			65	96	28	14
			42r	90	20	16
			55	95	35	21
Test Chemical	n-Butyl chloride	300	64	88	7	4	7
			66	76	19	10	7
		350	100	92	21	7	8
			90	113	26	10	8
		400	70	39	8	4	6
			82	49	22	9	6
		450	68	75	24	12	12
			83	59	31	12	12
		500	56	78	20	12	11
			74	36	22	10	11
		550

Positive Control	3-Methylcholanthrene	2.5	45	34	106	79	78*
Positive Control			39	41	91	77	78*

Induced S9 Trial: 2 Experiment Call: Rejected
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	76#	103	25.5	11	14
			89	106	32	12
			79	91	45	19
Test Chemical	n-Butyl chloride	250	75#	60	40.5	18	11
			72	75	9	4	11
		300	65	58	21	11	10
			77	43	20	9	10
		350	82	64	19	8	6
			85	56	13	5	6
		400	73	37	31	14	11
			80	50	18	7	11
		450	66	23	16	8	7
			82	42	14	6	7
		500

Positive Control	3-Methylcholanthrene	2.5	49	33	87	60	60*
Positive Control			64	41	117	61	60*

Induced S9 Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	81	108	38	16	22
			75	89	41	18
			84	103	74	29
			86	100	68	26
Test Chemical	n-Butyl chloride	250	81	52	55	23	19
			70	64	32	15	19
		300	76	56	53	23	22
			72	57	44	20	22
		350	69	63	64	31	26
			74	66	45	20	26
		400	71	54	36	17	17
			84	58	45	18	17
		450	38	26	36	32	26
			62	29	37	20	26
		500

Positive Control	3-Methylcholanthrene	2.5	42	18	356	283	271*
Positive Control			48	27	375	260	271*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination