Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-03110-0008-0000-5
Chemical Name:	o-Anthranilic acid
CASRN:	118-92-3
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	217797
Study Result:	Positive

View Study Details | View Protocol | Download To Excel

Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	88	106	176	67	66
			89	99	174	65
			89	99	186	70
			98	96	189	64
Test Chemical	o-Anthranilic acid	78	83	104	162	65	63
			87	119	160	61	63
		156	90	106	210	77	77
			77	95	177	77	77
		313	90	112	200	74	72
			83	97	174	70	72
		625	77	105	146	63	70
			63	83	143	76	70
		1250	93	93	237	85	89
			77	84	216	94	89
		2500

Positive Control	Ethyl Methane Sulfonate	500	5r	1	140	903	484*
			24	7	346	484
			6r	1	125	758

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	102	99	57	19	21
			88	107	56	21
			68	96	44	22
			74	98	51	23
Test Chemical	o-Anthranilic acid	125	96#	158	52.5	18	21
			99	159	70	24	21
		250	88	167	59	22	21
			102	170	60	20	21
		500	101	141	35	12	13
			95	138	41	14	13
		800	102	108	40	13	16
			103	134	58	19	16
		1000	83	61	63	25	22
			95	82	51	18	22
		1200
			87	55	61	23
		1300

Positive Control	Ethyl Methane Sulfonate	500	38	15	704	626	988*
			24	19	725	993
			19	14	766	1344

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	103	109	85	28	33
			105	93	123	39
			100	92	108	36
			102	106	95	31
Test Chemical	o-Anthranilic acid	500	90	83	94	35	34
			98	91	97	33	34
		700	96	74	162	56	48
			97	78	117	40	48
		1000	105	64	200	63	68*
			110	50	239	73	68*
		1200	103	65	180	58	195*
			31	2	308	331	195*
		1400	77	30	263	114

		1600

Positive Control	3-Methylcholanthrene	5	48	15	444	309	334*
			47	18	481	340
			42	16	446	353

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	72	105	73	34	34
			84	77	103	41
			86	92	90	35
			110	125	87	26
Test Chemical	o-Anthranilic acid	250	93	85	162	58	56*
			79	74	131	55
			79	73	127	54
		500	78	95	105	45	55*
			78	93	147	63
			70	80	119	57
		700	114	92	138	41	50
			76	87	117	51
			69	69	118	57
		1000	77	76	156	68	81*
			66	77	127	64
			73	52	242	111
		1200	55	4	538	328	234*
			53	11	377	238
			78	28	315	135
		1400					152*
			79	35	401	170
			67	22	269	135
		1600


Positive Control	3-Methylcholanthrene	5	38	7	496	433	404*
			39	9	405	351
			43	12	554	428

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination