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Study Information
Study Attribute Value
CEBS Accession Number: 002-03110-0009-0000-6
Chemical Name: o-Anthranilic acid
CASRN: 118-92-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 879005
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Solvent 
 0         
 99 110 139 47 51
    86 97 121 47
    95 102 122 43
    84 91 164 65
Test Chemical o-Anthranilic acid 
712         
 105 106 191 61 66
    89 89 192 72
  
949         
 100 80 186 62 61
    84 81 148 59
  
1266         
 82 24 301 123 110*
    71 14 206 97
  
2250         
 2r 0 9 180
    4r 0 13 113
  
3000         
 4r 0 7 67
    1r 0 7 175
  
4000         
 2r 0 1 20
Positive Control Ethyl Methane Sulfonate 
500         
 46 39 1125 821 617*
    98 120 726 248
    42 36 990 783

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 58 89 75 43 49
    58 101 73 42
    55 74 111 68
    72# 137 93 43
Test Chemical o-Anthranilic acid 
903         
 49 14 35 24 40
    20 3 33 56
  
1004         
 36 46 43 39 44
    45 57 65 48
  
1115         
 45 52 38 28 47
    44 13 86 66
  
1239         
 45 48 69 51 49
    49 55 69 47
  
1377         
 33# 11 58.5 60 89*
    28# 15 97.5 118
  
1530         
   
Positive Control Ethyl Methane Sulfonate 
500         
 40 50 955 803 843*
    37 41 903 817
    30 29 813 908

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 73 112 61 28 26
    53 77 46 29
    75 111 47 21
Test Chemical o-Anthranilic acid 
419         
 70 111 134 64 75*
    62 101 159 86
  
524         
 55 95 121 73 80*
    57 103 149 87
  
655         
 61 100 184 100 89*
    70 108 161 77
  
819         
 57 84 86 51 55*
    63 95 113 60
  
1024         
 57 83 87 51 44*
    83 113 94 38
Positive Control 3-Methylcholanthrene 
5         
 47 56 247 176 162*
    54 60 230 143
    43 54 217 168

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 69 85 135 65 78
    69 97 152 74
    72 98 195 91
    95 120 236 83
Test Chemical o-Anthranilic acid 
797         
 91 103 580 213
    121r 139 537 148
  
886         
 79 82 348 148 189*
    79 75 549 231
  
984         
 92 78 573 209 195*
    79 85 426 181
  
1094         
 86 89 460 179 175*
    56 56 289 171
  
1215         
 67 64 390 195 188*
    66 73 362 182
  
1350         
 65 52 441 227 212*
    86 68 507 197
  
1500         
 61 31 591 323
Positive Control 3-Methylcholanthrene 
5         
 55 41 758 457 512*
    72 54 980 455
    44 25 831 625

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination