Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-03110-0009-0000-6
Chemical Name:	o-Anthranilic acid
CASRN:	118-92-3
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	879005
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Solvent	0	99	110	139	47	51
			86	97	121	47
			95	102	122	43
			84	91	164	65
Test Chemical	o-Anthranilic acid	712	105	106	191	61	66
			89	89	192	72	66
		949	100	80	186	62	61
			84	81	148	59	61
		1266	82	24	301	123	110*
			71	14	206	97	110*
		2250	2r	0	9	180
			4r	0	13	113
		3000	4r	0	7	67
			1r	0	7	175
		4000	2r	0	1	20
Positive Control	Ethyl Methane Sulfonate	500	46	39	1125	821	617*
			98	120	726	248
			42	36	990	783

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	58	89	75	43	49
			58	101	73	42
			55	74	111	68
			72#	137	93	43
Test Chemical	o-Anthranilic acid	903	49	14	35	24	40
			20	3	33	56	40
		1004	36	46	43	39	44
			45	57	65	48	44
		1115	45	52	38	28	47
			44	13	86	66	47
		1239	45	48	69	51	49
			49	55	69	47	49
		1377	33#	11	58.5	60	89*
			28#	15	97.5	118	89*
		1530

Positive Control	Ethyl Methane Sulfonate	500	40	50	955	803	843*
			37	41	903	817
			30	29	813	908

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	73	112	61	28	26
			53	77	46	29
			75	111	47	21
Test Chemical	o-Anthranilic acid	419	70	111	134	64	75*
			62	101	159	86	75*
		524	55	95	121	73	80*
			57	103	149	87	80*
		655	61	100	184	100	89*
			70	108	161	77	89*
		819	57	84	86	51	55*
			63	95	113	60	55*
		1024	57	83	87	51	44*
			83	113	94	38	44*
Positive Control	3-Methylcholanthrene	5	47	56	247	176	162*
			54	60	230	143
			43	54	217	168

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	69	85	135	65	78
			69	97	152	74
			72	98	195	91
			95	120	236	83
Test Chemical	o-Anthranilic acid	797	91	103	580	213
			121r	139	537	148
		886	79	82	348	148	189*
			79	75	549	231	189*
		984	92	78	573	209	195*
			79	85	426	181	195*
		1094	86	89	460	179	175*
			56	56	289	171	175*
		1215	67	64	390	195	188*
			66	73	362	182	188*
		1350	65	52	441	227	212*
			86	68	507	197	212*
		1500	61	31	591	323
Positive Control	3-Methylcholanthrene	5	55	41	758	457	512*
			72	54	980	455
			44	25	831	625

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination