Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-03113-0012-0000-3
Chemical Name:	o-Chloroaniline
CASRN:	95-51-2
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	960448
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	103	99	163	53	52
			107	86	160	50
			119	126	191	53
			111	89	179	54
Test Chemical	o-Chloroaniline	31.25	124r	113	201	54
			112	93	165	49
		62.5	94	92	196	70	56
			101	85	130	43	56
		125	117	97	249	71	65
			104	96	180	58	65
		250	118	70	242	68
			125r	71	257	68
		500	109	31	226	69	67
			110	36	213	65	67
Positive Control	Methyl Methane Sulfonate	15	39	19	378	322	314*
Positive Control			42	19	382	306	314*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	60	84	55	31	28
			83	119	77	31
			80	97	61	25
			97	100	74	25
Test Chemical	o-Chloroaniline	25	78	93	70	30	32
			89	83	89	33	32
		50	94	95	104	37	32
			77	76	65	28	32
		100	69	106	105	51	40
			71	86	60	28	40
		200	78	71	79	34	38
			71	70	92	43	38
		400	71	25	94	44	51*
			58	22	100	58	51*
Positive Control	Methyl Methane Sulfonate	15	30	27	174	196	209*
Positive Control			25	34	167	223	209*

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	63	100	256	136	106
			64	89	246	127
			86	114	187	73
			91	97	239	88
Test Chemical	o-Chloroaniline	200	44	41	182	139	141
			51	47	217	143	141
		300	49	42	376	258	218*
			44	40	237	178	218*
		400	58	38	519	300	310*
			57	40	551	320	310*
		500	53#	22	448.5	280	219*
			92	43	434	157	219*
		600	86	39	425	166	187*
			71	24	445	209	187*
		700

Positive Control	3-Methylcholanthrene	2.5	39	15	462	393	394*
Positive Control			34	16	407	395	394*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	77	84	69	30	27
			79	90	75	32
			81	125	46	19
Test Chemical	o-Chloroaniline	300	70	43	146	69	64*
			74	37	132	59	64*
		400	72	36	278	128	139*
			82	33	370	150	139*
		500	75	23	599	268	239*
			67	30	421	210	239*
		600	71	16	818	382	336*
			71	19	616	291	336*
		700	69	8	482	233	233*
			76	8	531	233	233*
Positive Control	3-Methylcholanthrene	2.5	44	30	350	266	264*
Positive Control			53	32	419	263	264*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination