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Study Information
Study Attribute Value
CEBS Accession Number: 002-03113-0012-0000-3
Chemical Name: o-Chloroaniline
CASRN: 95-51-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 960448
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 103 99 163 53 52
    107 86 160 50
    119 126 191 53
    111 89 179 54
Test Chemical o-Chloroaniline 
31.25      
 124r 113 201 54
    112 93 165 49
  
62.5       
 94 92 196 70 56
    101 85 130 43
  
125         
 117 97 249 71 65
    104 96 180 58
  
250         
 118 70 242 68
    125r 71 257 68
  
500         
 109 31 226 69 67
    110 36 213 65
Positive Control Methyl Methane Sulfonate 
15         
 39 19 378 322 314*
    42 19 382 306

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 60 84 55 31 28
    83 119 77 31
    80 97 61 25
    97 100 74 25
Test Chemical o-Chloroaniline 
25         
 78 93 70 30 32
    89 83 89 33
  
50         
 94 95 104 37 32
    77 76 65 28
  
100         
 69 106 105 51 40
    71 86 60 28
  
200         
 78 71 79 34 38
    71 70 92 43
  
400         
 71 25 94 44 51*
    58 22 100 58
Positive Control Methyl Methane Sulfonate 
15         
 30 27 174 196 209*
    25 34 167 223

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 63 100 256 136 106
    64 89 246 127
    86 114 187 73
    91 97 239 88
Test Chemical o-Chloroaniline 
200         
 44 41 182 139 141
    51 47 217 143
  
300         
 49 42 376 258 218*
    44 40 237 178
  
400         
 58 38 519 300 310*
    57 40 551 320
  
500         
 53# 22 448.5 280 219*
    92 43 434 157
  
600         
 86 39 425 166 187*
    71 24 445 209
  
700         
   
Positive Control 3-Methylcholanthrene 
2.5       
 39 15 462 393 394*
    34 16 407 395

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 77 84 69 30 27
    79 90 75 32
    81 125 46 19
Test Chemical o-Chloroaniline 
300         
 70 43 146 69 64*
    74 37 132 59
  
400         
 72 36 278 128 139*
    82 33 370 150
  
500         
 75 23 599 268 239*
    67 30 421 210
  
600         
 71 16 818 382 336*
    71 19 616 291
  
700         
 69 8 482 233 233*
    76 8 531 233
Positive Control 3-Methylcholanthrene 
2.5       
 44 30 350 266 264*
    53 32 419 263

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination